Gel Permeation Chromatography Basics and Beyond
GPC/SEC, Gel Permeation Chromatography or Size Exclusion Chromatography, is a non-interactive chromatographic technique that separates analytes based on their size in solution.
It is often used in laboratories for determination of a polymers' molecular weight distribution but has many other uses.
In this talk we will:
Review GPC theory
Discuss why molecular weight distribution is important
Look at the different types of columns and their uses
Cover key column selection criteria
Show how key parameters like concentration, particle size, and injection volume can affect your separation
We will also look at examples of common mistakes and pitfalls relating to solvent choice, calibration standards, and combining columns in series.
Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
