HPLC Method Development: From Beginner to Expert, Part 1

From beginner to expert, we can all benefit from a review of HPLC basics. In this two-part talk, we will work our way from basic chromatographic principles to advanced method development topics.

In Method Development 101, we will cover such fundamentals as partitioning, retention factor, selectivity, and resolution. After a quick review of these basics, we seek to better understand the resolution equation and what it tells us about establishing or changing method conditions. We will also discuss how the physical/chemical properties of our analytes impact our choice of column chemistries.

Presenter: Melissa Goodlad (Application Engineer, Agilent Technologies, Inc.)

Melissa Goodlad is an Application Engineer providing applications assistance and technical support for Agilent’s HPLC columns and consumables. Before joining Agilent, Melissa was completing her PhD in Physical Chemistry at UC Merced studying nanoparticle self-assemblies.