Leveraging innovative stationary phase architectures and multi-detection strategies in biopharma and proteomics research to enhance precision, efficiency, and results

The 1st key-note presentation will explore the pursuit of enhanced chromatographic performance in low-flow applications.

Microfluidic pillar array columns offer a compelling alternative to traditional packed beds and monolithic columns, resulting in microfluidic separation beds with nanometer-scale control over geometry, enhancing quantitation accuracy and precision. The presentation focuses on a 5.5 cm separation channel housing radially elongated rectangular pillar shapes, ideal for high-throughput chromatographic separations. Integrated with advanced LC-MS instruments, the Vanquish Neo UHPLC system, and the latest Orbitrap mass spectrometers, this configuration achieves unparalleled quantitation accuracy and precision, handling up to 300 samples daily.

The second part will take us to detection technologies. HPLC has seen the advent and often also decline of various techniques which were claimed to enable universal detection. The emphasis will be on the use of an instrumental technique that was published specifically for charged aerosol detection (CAD) and is referred to as inverse gradient with the goal to widely eliminate the influence of the mobile phase composition on the analyte response. Practical examples will address extractables & leachables, but also very common excipients in biopharmaceutical formulations. The method uses multiple orthogonal detectors (UV, CAD, MS) combined with the inverse gradient technique to facilitate analysis of a chemically diverse range of analytes.

Learning points:

• How to achieve unparalleled quantitation accuracy and precision in high-throughput chromatographic separations

• Detection technologies

Who should attend:

• Lab managers and directors

• Principal scientists

• Laboratory analysts