Advancing Top-Down Proteomics for Precision Medicine on the timsTOF Pro

To understand biological systems at a functional level, a comprehensive picture of the diverse forms proteins can take is crucial. Top-down mass spectrometry (MS)-based proteomics provides a comprehensive analysis of such proteoforms, but the extraordinary dynamic range of the human proteome has meant that the MS detection of low-abundance proteins has remained an unsolved challenge. New strategies in top-down proteomics for precision medicine enabled by the timsTOF Pro are however helping to change this.

In this webinar, David Roberts will present novel top-down proteomics and nanotechnology approaches for the sensitive enrichment, analysis, and characterization of low-abundance protein biomarkers.

Moreover, David will reveal a first-hand look at how the timsTOF Pro enables in-depth proteomics for the high-throughput proteomic analysis of 3D human induced pluripotent stem cell-derived engineered cardiac tissues assisted by a novel photo-cleavable surfactant, Azo. Lastly, David will demonstrate new native ion mobility MS approaches for the in-depth characterization of native antibody drug conjugates with high sensitivity.

In this webinar you will learn about:

• Top-down proteomics approaches for in-depth biomarker analysis toward precision medicine
• How rationally designed nanomaterials can enable the sensitive and specific enrichment of low-abundance proteins
• How TIMS enhances intact protein analysis and characterization and enables high-throughput, in-depth proteome coverage
• timsTOF Pro-enabled native MS characterization of antibody drug conjugates

Presenter: David S. Roberts (Chemistry PhD Student, Ying Ge Lab and Song Jin Lab, University of Wisconsin-Madison)