A Multitude of Separation Techniques Hyphenated to MS for Biopharmaceutical Characterization — Case Studies from a Development Lab
A broad range of separation techniques are now routinely combined with mass spectrometry for in-depth Biopharmaceutical characterization.
Simultaneously, MS software tools have evolved to support storage, processing and reporting of the expanding MS data oceans. Here recent progress, challenges, and learnings from MS-based workflows at Symphogen will be present and illustrated through case-studies.
Additionally, an overview of the technological highlights of a novel mono-disperse ion-exchange technology for both strong cation and anion exchanger will be presented. Applications for high-resolution charge profiling of biopharmaceutical modalities including charge variant analysis of monoclonal antibodies and recombinant proteins, and the determination of full and empty AAV viral vectors will be described.
Learning points:
Separation techniques for in depth biopharmaceutical characterization
MS software tools have evolved to support storage, processing and reporting of the expanding MS data
Novel mono-disperse ion-exchange technology for both strong cation and anion exchanger
Who should attend:
Lab managers and directors
Principal scientists
Laboratory analysts
Presenter: Shane Bechler (Senior R&D Manager, Thermo Fisher Scientific)
Shane Bechler Ph.D. is a Senior R&D Manager at Thermo Fisher Scientific based in Sunnyvale, California. Over the past 11 years, his work has centered on the development of chromatography columns with a primary focus on biomolecule separations. Prior to joining Thermo Fisher Scientific, Shane obtained B.S. and Ph.D. degrees in chemical engineering from the Georgia Institute of Technology and University of Wisconsin - Madison, respectively.
Presenter: Christof Mitterer (Global Product Manager for BioLC Columns and Consumables, Thermo Fisher Scientific)
Christof currently holds the position of Global Product Manager for BioLC Columns and Consumables within the columns and consumables business unit at Thermo Fisher Scientific. Before that, Christof was responsible for business development at PharmaFluidics, the Belgian company of silicon wafer-based µPAC columns, which was acquired by Thermo Fisher Scientific in 2021. Prior to joining PharmaFluidics, Christof started his professional career in 2015 as a sales representative for columns and consumables products within Thermo Fisher Scientific, covering South-East Germany and Austria. Christof holds a Master of Science in Biochemistry from the Technical University of Munich.
Presenter: Dan Bach Kristensen (Principal Scientist, Symphogen, a part of Servier)
Dan Bach Kristensen holds a Ph.D. in biology and B.Sc. degree in chemistry. Dan is specialized in protein chemistry and mass spectrometry, which he initially applied in the field of proteome research in Japan and later in Denmark. For the last 19 years Dan has been working with analytical development in the biopharmaceutical industry, on projects ranging from early discovery to product registration. Clinical indications include bleeding disorders, neutropenia, autoimmune diseases and oncology. Dan currently works as a Principal Scientist at Symphogen, a part of Servier, which specializes in the development of antibodies, antibody formats and antibody mixtures for the treatment of cancer and neuro inflammatory diseases
Presenter: Maria Grübner (Senior Product Applications Specialist, Thermo Fisher Scientific)
Maria Grübner finished her degree in Food Chemistry in 2011 and afterwards worked in research on 2D-LC regarding metabolomics, both at the Technical University of Munich. Since 2016 she is with Thermo Fisher Scientific in Germering, Germany, working in the Product Applications Team with a focus on method transfers as well as non-conventional LC workflows / multidimensional LC.
Presenter: Dr Ken Cook (EU manager BioPharma expert support group, Thermo Fisher Scientific)
Following 7 years of experience in academia, Ken moved to industry from a University lectureship in Biochemistry, at the University of Newcastle upon Tyne. Now with over 35 years’ experience with Thermo Fisher Scientific using a wide range of analytical equipment and software. His publications range from metabolomics, and proteomics to large biopharmaceutical drug analysis. Ken is the European manager for the BioPharma expert support group. This involves the support of Bio-Pharmaceutical applications to characterise protein and oligonucleotide-based bio-therapeutics. This involves ongoing collaborations with the Biopharmaceutical industry and academia. Characterization methods of Bio-therapeutics include monoclonal antibodies, peptides, AAV analytical development, and small and large RNA analysis including sequence mapping of mRNA by LC/HRMS.
