Understanding LC Column Attributes: A Practical Guide to Column Selection

Liquid chromatography requires the selection and manipulation of several components to be an effective tool in separating complex mixtures. One of the most impactful components is the column stationary phase, which is where the separation occurs. However, a wide variety of column options are available regarding base particles, ligands and column hardware, making it very confusing which column to select for a specific analysis. A deeper dive into the important attributes of the column stationary phase is needed to fully understand how a column can impact the separation of analytes.

This webinar will be the first in a series and will focus on the different aspects of a column, ranging from base particle to column hardware selection. Chromatographic examples will be provided to highlight the different column attributes, such as pore diameter and base particle composition. Guidance will be given regarding column selection for method development. Understanding the basic attributes of the column stationary phase can better inform decisions when selecting columns, as well as allowing scientists to create the most robust methods possible. This translates into better and more reliable end results.

Presenter: Kenneth Berthelette (Senior Scientist, Waters Corporation Chemistry and Consumables Group)

Ken earned his Bachelor's of Science degree in Professional Chemistry from Bridgewater State University, Bridgewater Massachusetts, in 2010. He has been with Waters Corporation since March 2012 and has worked on several product launches including CORTECS columns, UPC2 Torus and Trefoil columns, and the Atlantis Premier BEH Z-HILIC column. He changed job functions in 2021 and now supports Chemistry Aftermarket initiatives. His focus on applications and customer education has led to several seminars and webinars on topics including method development, method modernization and transfer, HILIC separation techniques, and LC system troubleshooting.