Systematic Method Development with MaxPeak Premier Columns: A Simple and Easy Protocol for Fast LC Method Generation

Creating new LC methods is not as straightforward as most analysts would like. Finding the right column, and subsequently choosing the correct approach to method development is challenging and takes a considerable amount of time. Having a dedicated and documented protocol for method development can help streamline this process, by dictating which columns and conditions are used for all new method development activities across a lab. This not only standardizes the process to improve traceability, but also allows novice users to develop methods by themselves, freeing up the expert to work on more challenging samples.

This webinar focuses on method development for LC systems using a systematic screening protocol coupled with highly reliable MaxPeak Premier Columns. This webinar will not only look at the protocol, but also highlight the importance of screening different column stationary phases, mobile phase pHs, and mobile phase strong solvent. A case study example will be shown using the systematic protocol to develop an LC method for a forced degradation sample of orlistat, a weight loss drug.

Presenter: Kenneth Berthelette (Senior Scientist, Waters Corporation Chemistry and Consumables Group)

Ken earned his Bachelor's of Science degree in Professional Chemistry from Bridgewater State University, Bridgewater Massachusetts, in 2010. He has been with Waters Corporation since March 2012 and has worked on several product launches including CORTECS columns, UPC2 Torus and Trefoil columns, and the Atlantis Premier BEH Z-HILIC column. He changed job functions in 2021 and now supports Chemistry Aftermarket initiatives. His focus on applications and customer education has led to several seminars and webinars on topics including method development, method modernization and transfer, HILIC separation techniques, and LC system troubleshooting.