Single-cell proteomics on the Orbitrap Astral MS for the exploration of cell heterogeneity

Single-cell proteomics by mass spectrometry can provide valuable insights into distinct cell states and signaling patterns present in a cell population. However, carrying out proteomics profiling from the limited amount of material encapsulated in an individual cell presents significant challenges.

Tremendous efforts have been made to optimize all aspects of single-cell proteomics by mass spectrometry, with the aim of minimizing losses during sample preparation and maximizing sensitivity of data acquisition. Nevertheless, continuous development is needed to increase both the quantifiable proteome depth per cell, the quantitative accuracy and precision of those measurements, and the number of cells measured per unit time.

This webcast will present recently-developed approaches, ranging from sample preparation and chromatographic separation to advanced data acquisition, data interpretation and visualization methods. Advances in instrumentation, including the novel Orbitrap Astral MS, and how to use both TMTPro-labeled and label-free approaches, will be presented, showcasing the application of these methods to biomedical questions, and providing an overview of the current technological state of the field.

Learn how to:

• Prepare single cells for analysis by mass spectrometry
• Improve the sensitivity and throughput of single-cell proteomics analysis
• Implement both label-free and labeled quantitative approaches to single-cell proteomics

Presenter: Dr. Erwin Schoof (Associate Professor, Department of Biotechnology and Biomedicine, Section for Protein Science and Biotherapeutics, Cell Diversity Lab, Technical University of Denmark)