Best Practices for Analysis of IVT mRNA Integrity using the Agilent Fragment Analyzer Systems

Reliable and robust analysis is essential throughout in vitro transcription (IVT) mRNA workflows, providing important quality information to help minimize risk and aid in the production of a consistent product. In this webinar, our expert speakers will discuss how the Agilent automated electrophoresis systems are ideal for this assessment, providing fragment sizing and mRNA integrity at a variety of checkpoints throughout the process.

Key learning objectives

• Learn about the quality control (QC) steps that can be performed by the Fragment Analyzer and how they can improve the IVT process, including:
  • Quality and size of the linearized plasmid size
  • Purity analysis of the IVT mRNA

Who should attend?

Researchers, lab managers, lab directors, QA/QC managers doing IVT mRNA analysis in biotherapeutics research and production.

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Presenter: Whitney Pike (Application Scientist, Agilent Technologies)

Whitney Pike joined Agilent in 2016 and is an application scientist with a focus on Agilent’s automated electrophoresis instruments. She played a pivotal role in the release of the Femto Pulse system as a scientist on the product development team. She completed her master's degree in Biology, with an emphasis on cellular, molecular, and developmental biology, before working at a core facility at Iowa State University, responsible for performing next-generation sequencing studies.

Presenter: Dr. Leslie Friedmann (Product Manager Parallel CE systems, Agilent Technologies)

Dr. Leslie Friedmann joined Agilent in 2017 as a Product Manager for the Automated Electrophoresis portfolio. She focused first on the ScreenTape portfolio and is now responsible for the parallel CE instruments. Her education includes a diploma in biology from the University of Freiburg, Germany, a PhD in biology from the University of Frankfurt, Germany and a post-doctoral fellowship at the University of Singapore in developmental biology using molecular biology and gene expression analysis techniques.

Presenter: Professor Harris Makatsoris (Professor Of Manufacturing Systems in the Department of Engineering, King’s College London)

Harris is Professor Of Manufacturing Systems in the Department of Engineering at King’s College London and the founder of Centillion Technology Limited, a UK based biotech specialising in the rapid scaling and manufacture of RNA-based therapeutics and vaccines. He has a process engineering background with 27-years experience in both industry and academia. In all his past and present roles, he is managing interdisciplinary projects that require integration and coordination. Harris is an expert in continuous RNA manufacturing. His work is focusing on addressing the challenges in the rapid design, development and scalable manufacture of RNA and other nucleic acids and has pioneered the application of flow technologies in RNA manufacture, of various types. His research has attracted over £26m and he is currently leading a unique multi-disciplinary, multi-million pound programme comprising both industry and academia, building the Biofoundry-in-a-Box (BiaB). The BiaB is a unique portable, multi purpose RNA microfactory designed for the rapid design and deployment of a range of therapeutics and vaccines, with Low and Middle Income Countries in mind. He has several patents in his name in the space of RNA manufacture and continuous bioprocessing. He is highly experienced in technology transfer to industry through a variety of commercial routes.

Presenter: Jemima Arnold (Editorial Assistant, SelectScience)

Jemima studied Cellular and Molecular Medicine at the University of Bristol, UK, and as a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.