2D HPLC of Monoclonal Antibody with Novel Hydrophobic Interaction coupled to Reverse Phase Desalting with MS de

Two Dimensional HPLC (2D-LC) is a technique where two orthogonal techniques are brought together in one system for the purposes of increasing peak capacity as well as providing additional information from complex mixtures and analytes.

In this presentation we will show the analysis of a mAB with a HIC first dimension followed by a RP/desalting column in the second dimension. The second dimension column will removes salts from the mobile phase which will then allow the eluent to be analyzed by an mass spectrometer without the salts that could cause issues with the MS source.

Presenter: Bob Giuffre (Application Engineer, Agilent Technologies, Inc.)

Bob Giuffre has been working with HP/Agilent for almost thirty years as an Application Scientist for the HPLC product line. During that time Bob has worked on applications ranging from small molecule pharmaceutics, to large molecule polymers, to monoclonal antibodies. Currently, Bob is working in the area of ultra-high pressure two-dimensional liquid chromatography as a means to increase peak capacity for complex formulations. Bob has authored a chapter on the use of Diode-Array Detection in Pharmaceuticals. He is currently writing a chapter on Troubleshooting Peptide and Proteins Analysis using LC-MS.

Presenter: Sandeep Kondaveeti (Application Scientist, Agilent Technologies, Inc.)

Sandeep is a Philadelphia native and currently works as an applications chemist responsible for developing Bio-pharma workflow applications with Agilent Technologies supporting bio-columns portfolio. Prior to joining Agilent, Sandeep undertook graduate studies at Temple University in Bioinorganic chemistry focused in developing Inorganic metal clusters based on Photosynthetic metalloenzymes and worked as Process Analytical Scientist for Janssen Pharmaceuticals through Eurofins.