SCIEX summit Day three: Quantitation and screening
Day three: Quantitation and screening
- Enhanced sensitivity and robustness deliver stringent quantitation for veterinary drug testing for meat
- See how intuitive, compliant-ready SCIEX OS software enables pharmacokinetic parameter monitoring with ease and reliability
- Understand the challenges and best practices for oligoneucleotide analysis by LC-MS
- Discover how exploiting fast acquisition rates can be used to identify and quantify over 2250 fatty-acids lipids
- Hear about the accurate detection and quantitation of trace-lelvel PFAS compounds
Agenda-at-a-glance
Welcome and introduction to quantitation and screening
Veterinary drug analysis: SCIEX 7500 system vs 7500+ system
- Biochemie Lab shares their expertise in veterinary drug analysis. With over 25 years of experience and strict adherence to Commission Regulation (EU) No 37/2010, Biochemie Lab processes more than 100,000 samples annually, providing comprehensive food and environmental testing.
- Learn how Biochemie Lab conducts veterinary drug testing with specialized veterinarians, covering a wide range of analyses, including residual veterinary drugs, anabolic substances, and microbiological tests.
- Discover how they overcome challenges in analyzing complex animal-origin matrices like meat and offal using the SCIEX QTRAP 7500 system.
- Explore their proficiency testing, method validation results, and ask questions directly from leading experts in the field.
Maximizing analytical potential and navigating changes in the validated laboratory with SCIEX OS software
- Having the right information available at every stage of the process is vital to bringing safe and effective therapies to market.. SCIEX OS software is a vital component of this process, from discovery to development. See how:
- Your pharma lab workflows can benefit from SCIEX OS software
- Central Admin Console enables comprehensive audit configurations for full traceability
- Professional services are available to assist in migrating from legacy Analyst software and change control support plansis
Approaches for the bioanalytical analysis of oligonucleotide therapies
- Oligonucleotide therapeutics are on the rise in drug development and regulatory submissions. These drugs face challenges with bioanalytical extraction and analytical detection due to their chemical backbone or other stability-enhancing modifications.
- Learn about bioanalytical challenges and best practices for oligonucleotide analysis
- Hear case studies in the quantitation of oligonucleotide therapies using different mass spectrometry platforms
- Gain insights into improved method development for obtaining validated assays for regulatory evaluations using high-resolution mass spectrometry in early-stage development
Fast scanning quantitative lipidomics analysis using the SCIEX 7500+ system
- A panel of >2250 lipids was used to demonstrate the power and capability of the SCIEX 7500+ system to sensitively identify and quantify lipids at the fatty acid level using a multiple reaction monitoring (MRM). HILIC-like chromatography provides class separation to mitigate inter-lipid class isobaric overlap, but means that the speed at which individual MRM transitions are measured is crucial to ensure good coverage and quantitative accuracy.
- The ~4X faster analytical speed of the SCIEX 7500+ system improves the quantitative performance of a large, broadly targeted lipid panel
- The number of lipid molecular species quantified with %CV < 20 increased 59% during a period of the highest
- MRM concurrency (n = 925) compared to the fastest speed recommended on the SCIEX 7500 system
The faster MRM acquisition rates improve peak shapes by increasing the number of data points across the peak and providing better resolution of near-eluting lipid isomers
From sample collection to data processing - PFAS program at State Hygienic Lab
- The State Hygienic Lab at University of Iowa had the PFAS program running for couple of years and is accredited and certified by the state, EPA, and UCMR5 program to test for PFAS in water samples. However, testing encompasses different environmental and biological matrices. This presentation will look at the program and focus on:
- Approaches from sample collection to data processing and reporting.
- Testing for PFAS in water matrices (drinking, raw, well, surface, and groundwater) based on EPA 537.1 and 533 FAS methods.
- New method for PFAS in biological samples.
Q&A roundtable: quantitation and screening
- Moderated by: Ian Moore, Sr. Technical Product Manager, Nominal Mass, SCIEX
- Panelists include:
- Roberto Riccio, Biochemie Lab
- Shane Karnik, Senior Laboratory Director, Aliri Bioanalysis
- Ebru Selen, Biologics Applications Scientist, SCIEX
- Paul Baker, Senior Scientific Liason, Lipidomics and Metabolomics, SCIEX
- Craig Butt, Senior Manager, Scientific Marketing, Applied Markets, SCIEX
Moderator: Ian Moore, Sr. Technical Product Manager, Nominal Mass, SCIEX
With over ten years in product development, Ian is the Senior Technical Product Manager for nominal mass LC-MS/MS systems at SCIEX. He has also served as a Technical Product Manager and Global Technical Marketing Manager. Ian began his SCIEX career as an Application Scientist, focusing on biologics bioanalysis and DiscoveryQuant software validation.
Before SCIEX, Ian was the Lead Analytical Scientist at Custom Biologics, overseeing LC-MS/MS method development and clinical trial analysis. He also served as a Senior Scientist at Affinium Pharmaceuticals, focusing on metabolic stability profiling and enzyme inhibition assays.
Ian holds a postdoctoral position at McMaster University, specializing in antibiotic resistance and enzyme inhibitor screening
Presenter: Roberto Riccio (Biochemie Lab)
Presenter: Shane Karnik (Senior Laboratory Director, Aliri Bioanalysis)
Presenter: Ebru Selen (Biologics Applications Scientist, SCIEX)
Presenter: Paul Baker (Senior Scientific Liason, Lipidomics and Metabolomics, SCIEX)
Presenter: Craig Butt (Senior Manager, Scientific Marketing, Applied Markets, SCIEX)
