Quantitative metabolomics with validated standard protocols on high-resolution mass spectrometers
Metabolomics, crucial for understanding biological interactions, faces challenges due to a lack of standardized protocols. Addressing this, the biocrates AbsoluteIDQ p400 HR kit offers a ready-to-use, quantitative protocol targeting 408 metabolites across 11 compound classes and includes calibration, internal standards, and quality controls.
This webcast will describe how use of this targeted quantitative approach has now been validated for the Thermo Scientific Orbitrap Exploris family of mass spectrometers. Assessment using NIST SRM 1950 and human plasma samples demonstrates accuracy and reproducibility. Its applications span high-throughput analyses for large cohorts, drug discovery, and biomarker research, notably in neuroscience and cancer.
You will learn:
- How targeted metabolomics can be applied on high resolution instruments
- How standardization in metabolomics improves reproducibility across experiments and laboratories
- How absolute quantification aids in data interpretation
Presenter: Stephen Dearth (Senior Applications Specialist, biocrates life sciences)
Stephen Dearth is a senior applications specialist at biocrates life sciences ag, specializing in LC-MS-based targeted metabolomics. He has over 10 years of experience in the life sciences and has been with biocrates since 2017 after completing his PhD in chemistry with a focus on untargeted metabolomics and data analysis at the University of Tennessee.
Moderator: Nikki Forrester (Freelance science, writer and editor)
Nikki Forrester is a science journalist who covers biology, natural history, climate, and the culture of academic research. She earned a Ph.D. in ecology and evolutionary biology in 2019.
