Analyzing mRNA and sgRNA with Advanced Enzymes, Mass Spectrometry, and Informatics
As the therapeutic potential of RNA expands beyond COVID vaccines, new mRNA drug candidates for personalized cancer treatments and CRISPR sgRNA complexes are emerging, offering solutions for genetic disorders and epigenetic modulation. Recent advancements in RNA analysis provide critical insights into drug design, stability, purity, and molecular integrity.
To analyze larger therapeutic RNAs by LC-MS, they must be broken down into smaller fragments. Novel RNA endonucleases, high-purity mobile phase reagents, and integrated data processing workflows now simplify and automate these tasks, making LC-MS assays more accessible. These innovations lower the adoption barrier for LC-MS, accelerating the development and market readiness of RNA-based therapeutics.
Waters is at the forefront of advancing RNA therapy development with innovative analytical tools and workflows designed for rapid and precise sequence confirmation and modification mapping.
This webinar will feature insights from Quality Assistance a leading European Contract Research Organisation (CRO) offering a range of services including mRNA and CRISPR sgRNA testing. In this discussion, Quality Assistance will highlight their implementation of Waters' latest RNA solutions and discuss the analytical benefits these new tools have provided.
In this webinar you will learn:
- A comprehensive set of tools for LC-MS analysis of large RNA molecules, including enzymes, reagents, and informatics tools
- Novel digestion protocols with new RNases for identity, purity, and modification mapping
- Method considerations for sgRNA and mRNA digestion and data analysis
Presenter: Dr. Thomas Menneteau (R&D Scientist, Quality Assistance)
Dr. Thomas Menneteau studied at the Université Paul Sabatier in Toulouse, France and defended his thesis on the characterization of proteasome complexes by mass spectrometry in Dr Schiltz lab. He joined Dr. Thalassinos & Prof. Tabrizi labs at University College London as a postdoc to study DNA repair complexes involved in Huntington disease by native mass spectrometry and crosslinking-MS. He joined Quality Assistance in 2021 as a R&D Scientist and he is involved in the development of LC-MS method for the analysis of biomolecules (proteins, mRNA, …).
Presenter: Christian Reidy (Principal Product Manager, Waters Corporation)
Christian Reidy is a Principal Product Manager at Waters Corporation, specializing in analytical solutions for cell and gene therapy applications including HILIC, AEX, novel enzymes, standards, and reagents. With over a decade of experience in analytical chemistry, including project management and chemistry account management, Christian supports the advancement and commercialization of innovative products that empower researchers and pharmaceutical companies to advance RNA development.
