Robust and high-resolution characterization of viral vector quality from development to release with light scattering techniques

Viral vectors continue to be critical in the development of new gene and cell therapies. However, in-depth characterization of these complex systems remains a challenge throughout the development and manufacturing process. Developing robust analytical methodologies to characterize multiple critical quality attributes (CQAs) for large heterogeneous viral vector systems can be challenging.

In this webinar, we will discuss how to implement high throughput dynamic light scattering (DLS) and electrophoretic lights scattering (ELS) workflows initial screening, along with and multi-angle light scattering combined with size exclusion chromatography (SEC-MALS) or field-flow fractionation (FFF-MALS) for in-depth simultaneous multiple CQA characterization of AAV, LV, and other gene delivery vectors. Additionally, we will explore how to implement light scattering into bioprocess unit operations for real-time CQA analysis. The CQAs for these techniques include size, size distribution, aggregation, concentration, stability, and capsid content.

Key Learning Objectives:

• How DLS/ELS rapidly characterizes size distribution, aggregation, particle concentration, and stability in a high-throughput workflow.
• How SEC/FFF-MALS characterized heterogeneous samples for high resolution size distribution, payload, titer, and aggregation.
• How real time MALS characterizes and quantifies viral vectors (e.g. AAV, LV) for titer, payload, stability, and purity during TFF, AEX, and other bioprocess unit operations.

Who Should Attend:

• Scientists working in the field of gene therapy characterization, formulation, and process development.
• Casual learner looking to understand more about gene therapy and light scattering solutions.
• Lab Managers who want to learn about state-of-the-art instruments that enhance their lab's analytical capabilities.

Presenter: Parker Lee, Ph.D. (Waters | Wyatt Technology)

Dr. Parker Lee joined Wyatt Technology in 2022 after 5 years of work in macromolecule analytical development for various startups. As a field application scientist he is responsible for working with customers to ensure that they can utilize Wyatt’s light scattering instrumentation most effectively to improve and accelerate their research.