Size Exclusion Chromatography of Biomolecules: Column Selection and Method Optimization

Size exclusion chromatography (SEC) is a noninteractive technique that separates solutes according to their molecular size in non-denaturing conditions. It is often used as the first step in isolation of a protein from a crude sample. SEC is a gold standard to determine aggregate and fragment impurities in biotherapeutics.

In this webinar, we will outline basic theory, principles, and practical guidelines on column selection for SEC method development. We will overview biotherapeutic aggregation and how method parameters such as flow rate, column length, injection volume, and pH and salt concentration influence SEC.

Presenter: Mohit Patel (Application Engineer, Agilent Technologies, Inc.)

Mohit Patel is part of the CSD Online AE Team. His primary focus is to support SMLC, Bio-Columns, and consumables.

Before joining Agilent, Mohit was an Assistant Professor of Chemistry at Temple University. Mohit developed his passion for science through his experiences as a faculty researcher and a mentor. Mohit actively taught, developed curriculum, and served as senior personnel, with a leadership position, on funded grants, mentored research projects, and co-coordinator of STEAM related outreach services.

Mohit has earned a Doctorate degree in Chemistry from Temple University in 2016.