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Separation Science is the leading online resource for methods, applications, troubleshooting and training in chromatography and mass spectrometry.
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How to Prepare Honey Samples for Efficient Analysis of Antibiotics

RECORD | Already taken place Th, 12.12.2024
This presentation will detail different approaches to sample preparation for the analysis of antibiotics from honey prior to analysis on LC-MS/MS. It'll compare different SPE techniques to traditional QuEChERS.
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Separation Science: How to Prepare Honey Samples for Efficient Analysis of Antibiotics
Separation Science: How to Prepare Honey Samples for Efficient Analysis of Antibiotics

By attending this webinar, you will:

  • Learn about method development steps undertaken to provide a robust and sensitive method for sample preparation of antibiotics in honey
  • Gain an understanding of the Biotage sample preparation workflow and the benefits of automation
  • Discover the challenges faced when developing a sample preparation method for antibiotics in honey

Event Overview

This presentation will detail different approaches to sample preparation for the analysis of antibiotics from honey prior to analysis on LC-MS/MS (Liquid Chromatography coupled to tandem Mass Spectrometry). It will compare different solid phase extraction (SPE) techniques to traditional QuEChERS and present Biotage's automatable cartridge SPE for QuEChERS clean-up.

We will also discuss method development steps undertaken to fully optimize different sample preparation procedures for effective matrix removal and optimal recoveries for a panel of antibiotics from honey. The presentation will cover challenges faced during the development stages along with actions taken to overcome the issues as we develop an efficient sample preparation method. We will highlight the benefits of automating the methods within a laboratory to free up analysts' time and ensure good reproducibility.

The presentation is aimed at laboratories that undertake food analysis and are interested in optimizing the clean-up of their food samples to produce accurate and reliable results. The focus will be on the best strategies used to fully remove matrix interferences while maintaining high recoveries and good reproducibility in honey testing.

Presenter: Thomas Smith (EMEA Applications Laboratory Team Leader, Biotage)

Thomas has been with Biotage since July 2022 and is supporting customers to streamline their sample preparation approaches and help them automate their manual, labour-intensive tasks in the laboratory. Prior to this, Thomas worked in a toxicology lab analyzing hair for drugs of abuse and alcohol markers. His main role was to automate a method to increase reproducibility and turnaround time to meet the growing sample numbers.

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