Optimizing AAV Analysis with 3 μm Monodisperse SEC Columns
Adeno-associated viruses (AAVs) are as small, replication-defective, non-enveloped viruses, serving as vectors for gene therapy. AAV vectors play a transformative role in delivering therapeutic genes with low immunogenicity, safety, and long-term transient expression. In AAV production, the separation of monomeric forms from aggregates is pivotal for optimal gene therapy outcomes. The success of AAV-based gene therapies relies on the purity and homogeneity of AAV vector populations, necessitating the monitoring of AAV aggregation for production and quality assurance.
This webinar will detail an effective approach for separating AAV monomeric capsids from high molecular weight species (HMWS) using an SEC column packed with 550 Å pore size, 3 μm monodisperse silica particles in hydrophilic-coated stainless-steel hardware.
The benefits? Baseline resolutions of AAV monomer and aggregates in less than 4 minutes, preserved resolution even for injection of large volumes and consistent separation across independent lots.
You’ll learn:
- The role of SEC in AAV characterization
- The advantages of monodisperse silica particles in SEC
- The impact of pore size on SEC performance of AAV separation
- How to leverage Important factors like flow rate that affect AAV separation using SEC
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Presenter: Ke Ma, PhD (Staff Scientist, Thermo Fisher Scientific)
Presenter: Ashleigh Kootz (Global Product Manager, Thermo Fisher Scientific)
