Purification and quality control of oligonucleotides

Oligonucleotides are highly important biopharmaceuticals with strongly increasing relevance. Due to the high similarity of impurities originating from the synthesis, however, purification can be challenging.

This seminar demonstrates a successful large scale purification of an oligonucleotide using anion exchange chromatography (AEX) as well as efficient quality control by applying ion-pairing reversed phase chromatography (IP-RP).

Content

• How to perform a large scale AEX purification: Design of preparative system and useful features
• Benefits and limitations using AEX for preparative purification
• What to consider for developing an IP-RP methods: selection of stationary phase, ion-pair reagent, and solvent conditions
• How to improve analytical results by utilizing bioinert column hardware

Who are the seminar for?

• Advanced and beginners interested in peptides and/or oligonucleotides.

Presenter: Dr. Ulrike Krop (KNAUER Wissenschaftliche Geräte GmbH)

Presenter: Dr. Daniel Eßer (YMC Europe GmbH)