Method development and scale-up in peptide purification

Peptides have become one of the most important biopharmaceuticals. The high variability related to chain length and sequence of amino acids offers multiple pharmaceutical applications, and leads to specific demands regarding the production and purification.

This seminar therefore aims to provide the knowledge required for development of peptide purification methods based on case studies of up-to-date targets like a GLP-1 agonist and a peptide hormone.

Content

• How to choose the ideal stationary phase: the most significant aspects to consider and the importance of extensive screening
• What to consider for method development: finding efficient conditions by appropriate selection of solvents, pH and buffers
• From overview to focused gradient: how to optimize a robust preparative method
• Linear scale-up: Understanding the principle, system design and sample collection

Who are the seminar for?

• Advanced and beginners interested in peptides and/or oligonucleotides.

Presenter: Julia Bartmann (YMC Europe GmbH)

Presenter: Dr. Yannick Krauke (KNAUER Wissenschaftliche Geräte GmbH)