Thermo Scientific SureQuant Quantitation Workflow: a new paradigm in quantitative proteomics
The adoption of targeted proteomics methodologies for routine applications has been hampered by poor workflow reproducibility, unreliable quantitation, and lack of standardized methods and reagents. With the advent of SureQuant internal standard-triggered targeted quantitation on our next generation Orbitrap mass spectrometers, a new paradigm in biologically focused proteomics is underway.
Integration of reagents, instrumentation and data processing is now offered with Thermo Scientific’s broad range of reagents and tools to support targeted analysis and quantitation. This includes a variety of SureQuant multiplex immunoprecipitation to targeted mass spectrometry (mIP-tMS) kits, and our new multipathway phosphopeptide workflow to enable simultaneous enrichment and absolute quantitation of signaling pathway proteins. Fully optimized and validated SureQuant acquisition templates completely connect our sample prep and reagents to Orbitrap mass spectrometers and ensure hassle-free, turnkey solutions with the highest level of reproducible and sensitive quantitation.
In this workshop we will review the latest additions to the SureQuant workflow portfolio and learn about specific examples from our customers to develop robust targeted solutions for a variety of applications.
Who should attend:
- Director, PIs & Managers of Proteomics labs
- Director, PIs & Managers of Biopharma labs
- Current users of Q Exactive technology & QTOF MS analyzing peptides & proteins
What you will learn:
- Overview of the SureQuant internal standard-triggered quantitation workflows which provides higher efficiency and improved sensitivity to support targeted proteomics applications
- Development of new validated reagents, methods, and data processing tools to allow absolute quantitation of protein targets
- Case studies utilizing the SureQuant targeted approach for sensitive, reproducible and precise quantification of plasma proteomes, host cell protein (HCP) contamination, and tumor signalling
Presenter: Aaron Gajadhar, Ph.D. (Strategic Marketing Specialist – Proteomics, Thermo Fisher Scientific)
Aaron received his PhD in Medical Biophysics at the University of Toronto. Following his graduate training, he completed a postdoctoral fellowship at MIT with Forest White at the Koch Institute for Integrated Cancer Research. In 2015, Aaron joined Merrimack Pharmaceuticals in Cambridge where he worked as a Senior Scientist in Early Discovery. Aaron joined Thermo Fisher Scientific in San Jose in 2017 and is involved in the development of new LC-MS technologies for the proteomics market.
