Advanced IP-RPLC-MS Strategies for High-Resolution Oligonucleotide Impurity Profiling
Overview
This webinar, co-hosted by Phenomenex and the Bio-Analytical Separation Science Group at Vrije Universiteit Brussel, will cover:
- The growing potential of oligonucleotide therapeutics and the importance of thorough impurity profiling.
- Challenges in detecting critical impurities such as shortmers and phosphodiesters (PO) during synthesis.
- Ion-pair reversed-phase liquid chromatography’s (IP-RPLC) mechanism of action for high-resolution impurity analysis.
- Evaluating the impact of column preheaters on analyte dispersion and method performance.
- The role of fluoroalcohols in enhancing LC separation and MS detection.
- Comparing bio-inert columns for improved impurity profiling outcomes.
Key Learning Points
- Understand the therapeutic mechanisms of oligonucleotides and the significance of base, ribose, and phosphate modifications.
- Recognize the analytical challenges in oligonucleotide impurity profiling, including shortmers and phosphodiester impurities.
- Learn how to develop and optimize IP-RPLC-MS methods for high-throughput analysis.
- Evaluate the influence of preheater effects and fluoroalcohols on separation and detection sensitivity.
- Compare the performance of bio-inert columns for impurity profiling.
Who Should Attend
- Oligonucleotide Researchers: Ideal for scientists involved in developing and characterizing oligonucleotide therapeutics.
- Analytical Chemists: Professionals focused on impurity profiling and method optimization using advanced chromatographic techniques.
- Biopharmaceutical Quality Control Experts: Valuable for those ensuring the purity, stability, and safety of oligonucleotide-based therapeutics.
- Contract Research Organizations
- Biopharmaceutical Innovator Companies
Speaker: Dr. Sebastiaan Eeltink (Professor and lead investigator of the Eeltink Laboratory at the University of Brussel & Editor of Analytical Science Advances at Wiley)
Dr. Sebastiaan Eeltink is a Full Professor and lead investigator of the Eeltink Laboratory at the University of Brussel and an Editor of Analytical Science Advances at Wiley. His team focuses on developing and designing novel analytics for the high-resolution profiling of biomolecules and biomolecular interactions using ultrahigh-pressure liquid chromatography (UHPLC) and multi-dimensional liquid chromatographic workflows with mass spectrometry (MS) detection. In addition, his team also manages the University’s proteomics platform which offers high-performance LC-MS proteomics profiling expertise and advise to academic and industrial collaborators.
Speaker: Quang-Dong Bui (PhD student at University of Brussels)
Quang-Dong Bui is currently a PhD student at University of Brussels under the supervision of Professor Sebastiaan Eeltink, where he develops analytical methods for key oligonucleotide projects and is an active scientist in the oligonucleotide and mRNA research community.
