Beyond Batch Methods: High Resolution Nucleic Acid and LNP Insights with Light Scattering Techniques

Light scattering techniques, are transforming the way we quantify and analyze nucleic acid (NA) and lipid nanoparticle (LNP) therapeutics, playing a critical role in development, RNA quality assessment, and quality control. This webinar will explore how combining multi-angle light scattering (MALS) with field-flow fractionation (FFF) or size-exclusion chromatography (SEC) provides high-resolution insights into critical attributes such as size distribution, NA integrity, overall stability, and payload encapsulation. 

Recent advances in SEC column technology has increased the quality and resolution of separations for complex mixtures of large NA constructs, as well as NA-protein complexes including Cas9 constructs. FFF-MALS allows for gentle, high-resolution separation, making it ideal for analyzing NA delivery systems like LNPs without compromising their integrity. Additionally, dynamic light scattering (DLS) and electrophoretic light scattering (ELS) enable rapid, automatable batch measurements, allowing for screening of factors such as size and surface charge.

Join us to discover the latest advancements in light scattering technology and learn how these powerful tools can accelerate pharmaceutical development and enhance your research.

Key Learning Objectives:

• Applications of SEC-MALS to in-depth NA distribution, conformation, and stability characterization
• An introduction of DLS/ELS characterization for NA and LNP formulation screening
• High resolution characterization of LNPs and NA delivery vehicles via FFF-MALS for size distribution, conformation, and payload

Presenter: Parker Lee, Ph.D. (Waters | Wyatt Technology)

Dr. Parker Lee is a Field Applications Scientist at Waters | Wyatt Technology, serving customers in San Diego and the surrounding areas. Dr. Lee received his Ph.D. in Macromolecular Science & Engineering, with a focus on developing new methods for drug delivery. He joined Wyatt Technology in 2022 after five years of work in macromolecule analytical development for various startups. As a Field Applications Scientist, he is responsible for working with customers to ensure they can utilize Wyatt’s light scattering instrumentation effectively to improve and accelerate their research.

Presenter: Lavelay Kizekai, Ph.D. (Waters Corporation)

Lavelay Kizekai, BS, is a principal scientist at Waters Corporation. He joined Waters in 2009 and has since applied himself to developing new LC methods, leading column technology product development work, and establishing insight-based QC procedures for Waters products. His primary area of focus is biomolecule separations using size-exclusion chromatography (SEC). He was a core member of the team that recently brought to market a column suited to platform methods for monoclonal antibodies and antibody-drug conjugates. He is currently leveraging these SEC technologies and even newer breakthroughs for the characterization of gene-based therapeutics by multiangle light scattering.