Optimizing Peptide Mapping Chromatographic Separation
Overview
Participants will gain a clear understanding of which critical attributes to consider when selecting a column for peptide mapping and take home a set of method development tips to improve sequence coverage and sensitivity.
Key Learning Points
- Review Key parameters impacting separation
- Stationary phase chemistry
- Particle morphology
- Mobile phase organics and modifiers
- Column dimensions - miniaturization
- Learn about method parameter optimization (flow rate, column
- temperature, and gradient profiles)
- Deepen your understanding of stationary and mobile phase selection
Who Should Attend
- Those who perform routine analysis using gas chromatography and would like to begin exploring techniques for troubleshooting
Speaker: Lucia Geis Asteggiante (Senior Technical Specialist, Phenomenex)
Lucia is a Senior Technical Specialist that has recently joined Phenomenex’s Global Technical Team. She has a broad experience in the field of Analytical Chemistry with a particular focus on LC chromatography coupled with mass spectrometry. Lucia comes from a strong academic and regulatory background with experiences ranging from method development and analysis of small molecules to large protein complexes. She gained her Ph.D. at the University of Maryland - College Park, USA and did her post-doctoral work at the University of Oxford, UK. During her time as a researcher, she has authored/co-authored 23 scientific publications and 2 book chapters.
