Quantitative and Qualitative Solutions for Large-Panel Pesticide Assays using SCIEX Triple Quad and QTRAP Systems

This webinar covers different quantitative and qualitative strategies for the analysis of a large pesticides panel in complex food and beverage matrices like tea and fruit juices. Sample preparation varied with matrix complexity, ranging from simple dilution for orange juice to the inclusion of QuEChERS and dSPE clean-up for tea.

Data describing the quantitative performance of the SCIEX Triple Quad 4500 system for the analysis of 180 pesticides in tea will be presented. Specifically, key method performance parameters such as apparent recoveries, absolute recoveries, and matrix effects will be discussed to demonstrate the robust performance of the developed LC-MS/MS assay and how it was applied to the screening of real-world tea samples. While ion ratios and retention times are typically used as criteria for positive identification, alternative strategies such as MS/MS spectral library matching can also provide secondary confirmation.

The QTRAP technology of the SCIEX 5500+ system enabled data-dependent acquisition (DDA) of enhanced product ion (EPI) scans to produce MS/MS spectra for comparison against reference libraries to improve compound identification confidence. The resulting MS/MS spectral library searching workflow correctly identified >85% of the target pesticides in spiked orange juice.

By attending this presentation, you will learn about:

• Combined sample preparation and LC-MS/MS methods for large-panel pesticides screening in complex beverage matrices
• Both quantitative and qualitative data that demonstrates the robust performance of the SCIEX Triple Quad 4500 and SCIEX 5500+ systems for pesticides residue testing.

Who should attend:

• Food testing laboratories running residue testing with large analyte panels.

Presenter: Holly Lee (Staff Scientist, Food Market, Global Technical Marketing, SCIEX)

Holly completed her PhD with Prof. Scott Mabury at the University of Toronto, studying the biological and environmental processes involved in the fate of PFAS upon consumer disposal. Upon graduation, Holly worked for the Ontario Ministry of the Environment, Conservation and Parks as a senior analytical technologist before moving to SCIEX, where she spent the last 8 years working in mass spectrometry research and development, then transitioning to global technical marketing in food LC-MS/MS applications.