Mining the multi-ome
The presentation outlines the installation and utilization of the Orbitrap Astral mass spectrometer for multiomics analysis. Bart highlights the central dogma of biology, emphasizing the importance of integrating proteomics, metabolomics, and lipidomics to gain a comprehensive understanding of biochemical processes. The feasibility study involved analyzing various mouse tissues, demonstrating significant protein and metabolite identification. The presentation addresses challenges in multiomics, including sample preparation, methodology, and data processing.
The speaker showcases the advantages of fractionation and the use of advanced mass spectrometry techniques to achieve high-quality data. The presentation concludes with acknowledgments to team members and funding institutions, emphasizing the collaborative effort in advancing multiomics research.
Learning points:
- Comprehensive Multiomics Approach: Emphasizes the importance of integrating proteomics, metabolomics, and lipidomics to gain a holistic understanding of biochemical processes and disease models.
- Challenges in Multiomics Analysis: Highlights the difficulties in sample preparation, methodology selection, and data processing, and suggests fractionation as a solution to isolate different levels of metabolites, proteins, and lipids.
- Technological Advancements: Showcases the use of advanced mass spectrometry techniques, such as the Orbitrap Astral, for fast discovery and slow validation in multiomics research, ensuring robust and reproducible data.
Who should attend:
- Researchers in Biochemistry and Molecular Biology: Individuals interested in understanding the integration of proteomics, metabolomics, and lipidomics.
- Mass Spectrometry Specialists: Professionals looking to learn about the latest advancements and applications of the Orbitrap Astral mass spectrometer.
- Multiomics Data Analysts: Experts focused on overcoming challenges in data processing and methodology in multiomics research.
If you cannot attend this webinar please register to receive a link to the On Demand version the following day.
Presenter: Prof. Bart Ghesquière (Expert technologist, VIB - KU Leuven)
Bart Ghesquière is an expert technologist leading the VIB – KU Leuven Metabolomics Expertise Center since 2014. Trained as a biotechnologist at the University of Ghent, he worked on chromatographic technologies and mass spectrometry for studying protein modifications and N-glycosylation. He joined NIH in 2011 to quantify methionine oxidation in biological models, earning the Orloff Science award. In 2012, he applied his mass spectrometry expertise to angiogenesis research under Prof Peter Carmeliet.
Currently, he leads a team of 11 researchers, focusing on tracer metabolomics to profile metabolic disorders and develop technology. Bart Ghesquière is a Full Professor at KU Leuven and head of the Metabolomics Expertise Center at VIB.
