Untargeted Metabolomics and Lipidomics in Endometriosis

The presentation focuses on the multiomics analysis of endometriosis, highlighting the differences between targeted and untargeted approaches in metabolomics and lipidomics. The speaker emphasizes the challenges and advantages of untargeted analysis, which provides a broader picture but requires extensive data curation. The study involves analyzing plasma samples from endometriosis patients using advanced mass spectrometry techniques, including UVPD and HCD fragmentations, to identify and characterize metabolites and lipids. 

The presentation also discusses the importance of proper laser tuning in mass spectrometry for obtaining meaningful results. Preliminary data suggests that metabolic and lipid profiles could be useful for diagnosing endometriosis, despite challenges like overfitting in data models. The speaker acknowledges the collaborative efforts of team members and funding institutions, underscoring the significance of analytical chemistry in advancing multiomics research.  

Learning points:

• Targeted vs. Untargeted Approaches: The presentation discusses the differences between targeted and untargeted metabolomics and lipidomics, highlighting the broader scope and data-driven nature of untargeted analysis. 
• Analytical Techniques: Emphasizes the importance of advanced mass spectrometry techniques, such as UVPD and HCD fragmentations, for identifying and characterizing metabolites and lipids in endometriosis research. 
• Challenges and Solutions: Addresses challenges like data overfitting and the necessity of proper laser tuning in mass spectrometry, suggesting solutions like multiomics factor analysis and careful data curation. 

Who should attend:

• Biomedical Researchers: Individuals studying endometriosis or interested in the application of multiomics in disease research. 
• Mass Spectrometry Professionals: Experts looking to enhance their knowledge of advanced techniques like UVPD and HCD fragmentations in metabolomics and lipidomics. 
• Data Analysts in Life Sciences: Professionals focused on overcoming challenges in data curation and analysis in untargeted multiomics studies. 

If you cannot attend this webinar please register to receive a link to the On Demand version the following day.  

Presenter: Lukas Najdekr, Ph.D. (Senior researcher, IMTM Olomouc)

Lukas' research focuses on metabolomics. After studying biochemistry, he worked at University Hospital Olomouc, where his PhD was on applying untargeted metabolomics in clinical diagnostics. Internships at the University of Barcelona and Thermo Fisher Scientific provided him with skills in cell cultures, molecular biology, and Orbitrap-based mass spectrometry. He believes metabolomics has great potential as a universal diagnostic tool in personalized medicine, evident in innovations like the iKnife and improved newborn screening.

At Phenome Centre Birmingham, he gained insights into academia-industry cooperation. Now, at Palacký University, he aims to study oxidative stress at the metabolite level and advance lipidomics research in clinical practice, recognizing its significant diagnostic potential and research opportunities.