The Polar Puzzle: Solving Complex Analytical Challenges
Analyzing polar molecules presents unique challenges for chromatographers. These compounds span a wide range of applications from polar pesticides in food, to non-steroidal anti-inflammatory drugs (NSAIDs), to highly polar sugars and amino acids. Traditional HPLC stationary phases like C18 often fall short in retaining and separating such highly polar analytes. As a result, chromatographers must explore alternative polar chemistries, including reverse-phase polar columns and hydrophilic interaction liquid chromatography (HILIC) & normal phases.
In this webinar, we will provide practical guidelines for selecting the appropriate separation mode, HILIC, reverse phase (RP), or normal, based on analyte properties and application needs. We’ll walk through a structured checklist to help you make informed decisions when developing methods for polar molecule separations.
Key Takeaways:
- How to select the best column for retaining and separating polar analytes, with a focus on Reverse Phase & HILIC chemistries
- Choosing between Reverse Phase and HILIC based on analyte solubility and sample solvent
- Ensuring reliable detection through the right separation mode
- Selecting the appropriate column type (SPP or TPP) based on the application
- Method optimization strategies for improved results
Presenter: Dr. Sunil B Lakhmapure (Pre-Sales Application Engineer, Agilent Technologies)
Sunil holds 19 year plus experience in the analytical field with major exposure to LC/GC with Single Quad, Triple Quad and high resolution (QToF) mass spectroscopy. Started career in Pharma R&D as a scientist with hands on experience on high end instruments like LC/MS, XRD & NMR. Developed & published application notes using Chromatographic techniques on real world samples/issues at various levels.
Presenter: Mr. Andrea Leonardi (Product Specialist, Chemistries & Supplies, Agilent Technologies)
Andrea holds a master's degree in Pharmaceutical Chemistry and Technology form Universita’ degli Studi di Camerino in Central Italy. Since completing his studies, he has held various laboratory and research roles with pharmaceutical companies in Italy and Switzerland, before coming to Singapore to join Novartis Institute for Tropical Diseases. This latest job involved characterizing novel APIs (Active Pharmaceutical Ingredients) by LC and LC/MS.
In Agilent, Andrea is a Product Specialist in the Chemistries and Supplies Division in the South-East Asia Pacific and Korea region. He supports customers by helping to develop new analytical methods and improve existing ones to enhance lab efficiency and productivity. He also conducts training, seminars, and workshops. His expertise is in LC and LC/MS analysis of small molecules and biologics.
