Method Development and Optimization of Native RPLC-HRMS Characterization of ADCs using Biozen Native RP Columns

During this presention, we will review the following:

• An RPLC-HRMS method development approach for rapid, comprehensive DAR analysis, including positional isomers, under native conditions.
• Sorbent polarity and hydrophobicity effects on retention and selectivity of individual DAR species.
• Optimized chromatographic separation and MS identification of all DAR species, including positional isomers, of several ADCs.

Key Learning Points

• Learn about method development approach for native RPLC-HRMS of ADCs using Biozen Native RP columns.
• Take a deep dive into method optimization strategies.
• Examine online HRMS identification of individual DAR species.

Who Should Attend

• Characterization and In-process scientists needing to develop robust, MS friendly methods to characterize and identify individual DAR species, including positional isomers, and average drug to antibody ratios (DAR) of antibody drug conjugates (ADCs).

Speaker: Carl Sanchez (Principal Scientific Advisor, Phenomenex)

Carl Sanchez is a Principal Scientific Advisor in Phenomenex R&D where he provides subject matter expertise and advanced technical problem-solving support to Phenomenex businesses and customers. Additionally, Carl is actively involved in new product development projects spanning HPLC, sample preparation and GC. Carl has authored multiple papers on HPLC in peer reviewed journals as well as given over 100 lectures at scientific conferences worldwide. Carl’s current research interests include LC-MS characterization of biotherapeutics.