Fresh solutions to elucidate the structure-function relationship of macromolecules
Discovery advancements in structural biology applications, including charge detection approaches including Direct Mass technology mode, protein cross-linking, and hydrogen-deuterium exchange (HDX). These technologies provide complementary information about the structure of proteins and RNA molecules, offering insights into their functional states. This session delved into how these innovative methods are enhancing our understanding of molecular structures, contributing to more accurate models of protein and RNA behavior, and driving progress in fields such as biochemistry and molecular biology.
Learning points:
- How native/charge-detection (Direct Mass) MS determines accurate mass and stoichiometry of intact protein complexes and other large macromolecules.
- How hydrogen–deuterium exchange (HDX-MS) maps conformational dynamics and ligand binding using rapid quench/online digestion for sensitive, high-throughput analysis.
- How cross-linking MS provides distance restraints to define intra-/inter-protein contacts and improve structural models alongside cryo-EM/X-ray.
Who should attend:
- Structural biologists and biochemists seeking complementary tools to cryo-EM/X-ray for dynamic or membrane proteins and RNA.
- Mass spectrometry method developers implementing native MS/Direct Mass, HDX-MS, and cross-linking workflows with strong QC.
- Drug discovery and computational biology teams needing reliable protein–ligand interaction and conformational insights to guide design.
If you cannot attend this webinar please register to receive a link to the On Demand version the following day.
Presenter: Weijing Liu Ph.D. (Vertical Marketing Manager, Thermo Fisher Scientific)
Presenter: Malvina Papanastasiou (Group Leader, Broad Institute)
Presenter: Varun Gadkari Ph.D. (Assistant Professor, University of Minnesota)Presenter: Philip Lössl (Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin)
