Imaging of intact proteoform complexes in tissue

Dr. Hale's research integrates native ambient mass spectrometry (NAMS) to analyze intact proteoform complexes in tissue samples under non-denaturing conditions. Utilizing nano-DESI (nano spray desorption electrospray ionization), this technique enables the spatial mapping of proteoforms within tissues, revealing their distribution and functional implications. The method involves home-built ion sources that provide high-resolution imaging compatible with advanced mass spectrometers like the Orbitrap Eclipse.

The presentation highlights the ability to analyze proteoforms and their post-translational modifications (PTMs) directly from tissue samples without extensive preparation, preserving the native state of protein complexes. This approach is particularly useful for studying neurodegenerative diseases, where understanding the spatial distribution and interactions of proteoforms can provide insights into disease mechanisms. Dr. Hale emphasizes the importance of this technology in advancing pathology research and improving the detection and identification of high molecular weight proteins and membrane protein complexes.

Learning points:

• Spatial Distribution Analysis: Nano-DESI mass spectrometry enables the spatial mapping of proteoforms within tissues, providing insights into their distribution and functional roles, which is crucial for understanding disease mechanisms, particularly in neurodegenerative diseases. 
• Non-Denaturing Conditions: The technique preserves the native state of protein complexes during analysis, allowing for the study of intact proteoforms and their post-translational modifications (PTMs) directly from tissue samples without extensive preparation. 
• Advanced Mass Spectrometry Techniques: Utilizing high-resolution mass spectrometers like the Orbitrap Eclipse, the method allows for detailed analysis of high molecular weight proteins and membrane protein complexes, enhancing the detection and identification capabilities for complex biological samples

Who should attend:

• Pathology Researchers: This technique provides valuable insights into the spatial distribution and interactions of proteoforms within tissues, aiding in the understanding of disease mechanisms and pathology. 
• Neurodegenerative Disease Scientists: The ability to analyze intact proteoform complexes in their native state is particularly beneficial for studying neurodegenerative diseases, offering potential breakthroughs in understanding disease progression and identifying therapeutic targets. 
• Mass Spectrometry Technologists: Professionals working with advanced mass spectrometry techniques can benefit from the innovative application of nano-DESI for high-resolution imaging, enhancing their analytical capabilities and methodologies for complex biological samples.  

If you cannot attend this webinar please register to receive a link to the On Demand version the following day. 

Presenter: Dr. Oliver Hale (Postdoctoral Research Fellow, University of Birmingham)

Dr Oliver Hale is a postdoctoral research fellow who specializes in mass spectrometry. His current focus is on combing native mass spectrometry analysis of intact proteins and mass spectrometry imaging, as part of the EPSRC-funded NAMS project.