Deep Visual Proteomics

Discover how cutting-edge imaging and LC-MS technologies combine to reveal protein expression patterns with unprecedented depth, precision, and biological context.

Exceeding 100 spatially-resolved proteomes per day: An optimized ultrasensitive tissue proteomics workflow

• Talk by Melissa Klingeberg, PHD Student at Max Delbrück Center, Spatial Proteomics Group, Berlin

Spatial proteomics (SP) enables precise mapping of protein abundance, localization, and interactions in tissues, offering deep insights into cellular function and disease. We co-developed Deep Visual Proteomics (DVP), integrating high-resolution microscopy, AI-driven image analysis, and laser microdissection with deep proteomic profiling. Here, I will present an optimized cellenONE protocol for loss-minimized tissue processing, benchmarked across all Evosep ONE Whisper Zoom gradients and three DIA methods on the timsUltra AIP. Our results demonstrate the feasibility of acquiring over 100 high-quality spatial proteomes per day—paving the way for large-scale, translational SP studies.

More SPEAKERS and talks to be announced