Approaches for Improving Purification Outcomes for Polar Compounds

Reversed-phase HPLC is often the method of choice for the majority of purification scientists. Complex sample mixtures frequently contain many classes of compounds with both polar and non-polar moieties. Polar compounds present a unique problem in LC purification. While the non-polar C18 stationary phase is often the column of choice for most isolation work, its perceived universality for separating mixtures of diverse compounds frequently fails to retain polar compounds. Although many different strategies exist for tackling this problem, specially developed stationary phases designed for enhanced polar compound retention may be used as the column of choice for the purification of all targets, including nonpolar ones.

Come and join us as we discuss approaches for successfully navigating the complexities of polar target retention and isolation in the purification laboratory. 

Presenter: Jo-Ann Jablonski (Principal Scientist, Waters Corporation)

Jo-Ann Jablonski is a Principal Scientist in the Consumables and Lab Automation Group at Waters Corporation. Prior to joining Waters, she worked in biotechnology synthesizing, characterizing, and purifying biomolecules for research and development. She later transitioned to developing a process for the manufacture of a therapeutic peptide product. Since joining Waters, Jo-Ann has provided technical support, training, and application assistance to customers using Waters’ chemistry products and instrumentation, with an emphasis on purification technologies.