Optimization and Validation of the Kairos Amino Acid Kit in a hospital laboratory

The accurate, precise and reproducible measurement of amino acids is critical in the detection and monitoring of inherited metabolic disorders such as PKU, MSUD, UCDs etc. Waters' Kairos Amino Acid offers mass spectrometry based detection that is accurate, precise and enables the quantification of 40+ amino acids. 

In this webinar, Marijn Van Hulle from Waters will provide an overview of the existing Kairos Amino Acid kit as well as a preview of upcoming optimziations to the kit's method. Additionally, Kiara Theron from McMaster Children's Hospital will present the outcome of her validation studies of the Kairos Amino Acid Kit in plasma, urine & CSF patient samples.

Learning points:

• Description of the kit & overview of the AccQTag derivatization chemistry
• Advantages of the status quo Kairos Amino Acid Kit 
• Overview of the optimization changes for the Kairos Amino Acid Kit
• Validation of the Kairos Amino Acid kit in plasma at McMaster Children's Hospital

Who should attend:

• Lab Scientists/Managers/Directors in General Hospitals or Children Hospitals and Reference Labs interested in developing and validating an LDT for Amino Acid analysis for routine clinical care 

Presenter: Kiara Theron (Research Assistant & Master's student at McMaster University Pathology & Molecular Medicine, McMaster Children's Hospital)

Kiara Theron is a second year Master’s student in McMaster’s Medical Sciences graduate program. She is currently working under Dr. Murray Potter at McMaster Children’s Hospital to optimize and validate LC-MS/MS methods for biochemical monitoring in patients with inherited metabolic disorders. Her more recent work has focussed on amino acid analysis for these patients as part of which she optimized and validated the Kairos Amino Acid kit for plasma, and urine, and CSF samples against the lab's predecessor method which was based on the MassTrak Amino Acid Kit.

Presenter: Marijn Van Hulle (Global Director for Clinical Applications & Support, Water Corporation)

Marijn Van Hulle is the Global Director for Clinical Applications & Support. He oversees field applications, global escalations of chemistry, IVD kit and consumables support, method transfers, and the application notes pipeline in collaboration with R&D. Marijn joined Waters in 2004 as an LC-MS application specialist in the Benelux after earning his PhD in analytical chemistry from Ghent University. He has (co)authored nine Waters application notes and four peer-reviewed papers.