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Tips and tricks to select HPLC columns

RECORD | Already taken place We, 2.12.2020
Ion suppression is a major issue during LC-MS method development where C18 columns are usually used. Sometimes it’s necessary to think out of the C18 box to avoid this.
Go to the webinar
Thermo Scientific Webinars: Tips and tricks to select HPLC columns
Thermo Scientific Webinars: Tips and tricks to select HPLC columns

This webinar will show:

  • Common ion suppression issues related to analytes and their logP`s as well as matrix dependence
  • Solutions to move analytes out of ion suppression fronts by changing the HPLC column selectivity
  • Overview of certain selectivity’s and how to choose one
  • How to transfer a method from spherical silica to core shell materials
  • Solutions to injection related issues
  • Other causes of ion suppression during method development
  • What else has an influence on the results
  • General tips and tricks on how to avoid commonly seen issues

Learning points:

  • What causes ion suppression and how to move an analyte out of this front
  • What selectivity’s are available outside the C18 box
  • How to create a method development

Who should attend?

  • LC and LC-MS method developers
  • LC-MS users
  • Scientists with challenging situations in LC-MS analysis

Presenter: Petra Gerhards (Regional Marketing Manager EMEA CCS, Thermo Fisher Scientific)

Her background is over 30 years of experience in the field of GCMS, SPE and LCMS. Since joining the EMEA Regional Marketing Team for CCS she has been involved in a lot of workflow solutions combining Vials and Closures with SPE solutions, GCMS and LCMS. She works with KOL`s on data for regional specific marketing campaigns, organizes inhouse seminars and works on customer specific solutions. Her main expertise is in the field of Doping and Drugs of Abuse analysis.

Presenter: Tim Cross (Sr. Manager, Regional Marketing, Thermo Fisher Scientific)

Thermo Fisher Scientific
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