Controlling Retention Factor When Investigating Oligonucleotide Chromatography
Oligonucleotide therapeutics are a rapidly emerging focus in the pharmaceutical industry and represent a significant analytical challenge. The high degree of complexity necessitates high-performance separation methods, and 2D-LC has begun to be employed in the elucidation of impurity profiles in early phase discovery.
We outline the critical impact of pressure on the retention factor of oligonucleotides and the subsequent need to control for retention factor when investigating the fundamental mechanisms behind optimizing the separation performance of oligonucleotides. We will describe the various method development tools we employ to probe the impact of various chromatographic parameters (gradient slope, complementarity, flow rate, pore size, etc.) as a function of retention factor.
By viewing this presentation, you will:
- Understand why controlling retention factor is critical for reliable oligonucleotide separations.
- See how chromatographic parameters like gradient slope, pore size, and flow rate influence performance.
- Learn how 2D-LC enhances impurity profiling in early-phase oligonucleotide discovery.
Presenter: Daniel Meston (Associate Research Professor at Gustavus Adolphus College, USA)
Daniel Meston is an Associate Research Professor at Gustavus Adolphus College, USA. Daniel's research focus is on the development of bioanalytical separations, primarily of peptides and oligonucleotides by online 2D-LC. Outside of research, Daniel is the ChromSoc Vice President and the editor of the publication ChromCom.
