Advanced RP-HPLC Techniques for Peptide Analysis: From Column Chemistry to Mobile Phase Modifiers

Reversed-phase high-performance liquid chromatography (RP-HPLC) is a cornerstone technique for peptide analysis due to its exceptional resolving power. In this webinar, we will explore how to optimize RP-HPLC parameters for effective separation of peptides derived from protein digests and analysis of PTMs analysis. Key topics include the role of stationary phase chemistry in influencing selectivity, and how column physical characteristics affect separation performance. We will also examine how mobile phase modifiers—such as trifluoroacetic acid (TFA), formic acid, and pH—impact chromatographic outcomes and compatibility with mass spectrometry (MS). Attendees will gain practical insights into method development strategies that enhance both resolution and detection sensitivity.

Presenter: Mohit Patel (Application Engineer, Agilent Technologies, Inc.)

Mohit Patel is part of the CSD Online AE Team. His primary focus is to support SMLC, Bio-Columns, and consumables.

Before joining Agilent, Mohit was an Assistant Professor of Chemistry at Temple University. Mohit developed his passion for science through his experiences as a faculty researcher and a mentor. Mohit actively taught, developed curriculum, and served as senior personnel, with a leadership position, on funded grants, mentored research projects, and co-coordinator of STEAM related outreach services.

Mohit has earned a Doctorate degree in Chemistry from Temple University in 2016.