Accelerating GLP-1 Analog Impurity Profiling with the Agilent Pro IQ Plus Followed by Detailed Sequence Analysis using Electron Capture Dissociation

In biotherapeutic development, speed and confidence in impurity profiling are critical to moving candidates forward. This webinar introduces a streamlined workflow designed to accelerate impurity profiling of GLP-1 analogs. It begins with impurity screening using the new Pro IQ Plus single quadrupole mass detector. Then, detailed sequence analysis is performed using the 6545XT AdvanceBio LC/Q-TOF, equipped with an ExD cell to enable electron capture dissociation (ECD). ECD offers a gentler fragmentation approach than traditional CID, preserving labile modifications and enabling detailed structural insights. 

We highlight key advantages of ECD including modification localization, isoaspartate investigation, and discuss the importance of spectral quality in sequence confirmation. Whether you're optimizing GLP-1 analogs or exploring new biotherapeutics, this webinar highlights how Agilent’s tools can help get answers faster and go deeper with your data.

Presenter: Rachel Franklin (Application Development Scientist, Agilent Technologies, Inc.)

Rachel is an Application Development Scientist at Agilent Technologies who specializes in developing innovative applications using high resolution mass spectrometry. She joined Agilent in 2023 through the acquisition of e-MSion, the developer of the core technology for the Agilent ExD cell. Rachel earned her PhD in Biochemistry and Biophysics from Oregon State University where she focused her research on studying genetically engineered protein systems using top-down mass spectrometry. In her current role at Agilent, Rachel continues to advance research in this area and enjoys exploring new application areas.