Sample Preparation Strategies for Oligonucleotides Bioanalytical Workflows
Synthetic oligonucleotides represent a growing therapeutic modality. One analytical challenge in development is supporting in vivo studies. In particular, sample preparation and extraction of oligos from various biological matrices can be quite complex. Typical workflows use a mixture of liquid-liquid extraction and solid-phase extraction (SPE) to isolate the oligo, followed by analysis by tandem mass spectrometry. This approach can be successfully implemented for many therapeutic platforms such as anti-sense oligos (ASOs) and silencing RNAs (siRNAs). However, depending on the type of biological matrix (e.g., tissue type), the standard sample preparations may not have the sample recovery nor cleanup necessary for sensitivity requirements of the method. Further, depending on the type of oligo, even established methods such as mixed mode SPE simply may not be feasible.
In this webinar, expert Brian Rivera from Phenomenex will give an overview of sample preparation strategies for synthetic oligonucleotides, including how to adjust experimental design depending on the oligo and sample matrix. The presentation will also go into depth about the critical parameters to adjust to ensure effective sample preparation and cleanup methods are implemented. Additionally, other approaches for other challenging oligos, such as peptide nucleic acids or morpholinos — which are unique in that they do not have the negatively charged backbone associated with other nucleic acid chemistries — will be discussed.
Key learning objectives:
- Optimization of sample pretreatment and solid-phase extraction for different types of oligos
- How to approach extraction depending on the biological matrix you want to extract your sample from, including solid tissue
- Alternative sample prep strategies using hybridization extraction
- How to utilize chromatography to improve method sensitivity
Who should attend?
- Bioanalytical chemists new to working with oligonucleotides
- Bioanalytical scientists needing to improve method sensitivity
- Anyone new to oligonucleotide characterization by LC-MS
Presenter: Brian Rivera (Global Product Manager for Bioseparations Products, Phenomenex)
Mr. Brian Rivera is currently the product manager for bioseparations products at Phenomenex, Incorporated, Torrance, California, USA. Prior to this role, Brian held a variety of positions in sales and marketing related to biotechnology products, with previous roles including technical sales specialist at Phenomenex and Eppendorf, account manager at ProZyme and biotechnology product specialist at Phenomenex. Prior to these business-facing roles, Brian worked in analytical methods development and in manufacturing support roles for several biotechnology companies in the San Francisco Bay Area; including Avid Bioservices, ProZyme, and Chiron Corporation. He has a bachelor’s degree from the University of California, Davis.
Moderator: Finn Price (Editorial Team, SelectScience)
Fionnbhar Price is a member of the SelectScience Editorial Team, who plays a core role in sourcing and publishing content for the site, with a particular focus on the field of applied chemistry. He has an MChem in Chemistry from Cardiff University, UK, and undertook a year in research characterizing bacterial protein.
