Size variant analysis of antibodies and ADCs using SEC, SEC-MALS, SEC-MS, and 2D Protein A-SEC Methods

Advanced Size Variant Analysis of Antibodies and ADCs Using SEC Techniques

Size‑exclusion chromatography (SEC) is essential for analyzing aggregates, monomers, and fragments in biotherapeutic proteins, but traditional methods often require extensive optimization due to non‑desired ionic and hydrophobic interactions. Learn how these innovations improve resolution, reduce eluent complexity, support SEC-MALS, SEC-MS, 2D Protein A -,SEC workflows, and deliver robust, out-of-the-box performance across a wide range of protein modalities.

• Review SEC fundamentals and smart column selection
• Build robust, platform-ready SEC methods for mAbs
• Leverage advanced MALS and MS detectors for deeper insight
• Direct analysis of mAb HMWS in cell-culture with 2D Protein A- SEC

This webinar is designed for Scientists in Biopharma, CXOs, Academic, Government performing SEC-based protein separations.

Speaker: Stephan M. Koza, Ph.D. (Senior Principal Scientist, Waters Corporation)

Stephan Koza has worked at Waters Corporation for over 10 years and has a primary focus on the use of LC, LC-MS, and sample preparation chemistries and columns for the analysis of biomolecules.

Prior to joining Waters, he had nearly twenty years of experience with biopharmaceutical characterization and analytical method development with Genetics Institute, Wyeth, and Pfizer.

Stephan received his doctorate in analytical chemistry at the University of New Hampshire.

Speaker: Bill Warren (Principal Consultant Product Manager, Waters Corporation)

Bill joined Waters in 1986 as a senior applications chemist involved with the research and development of HPLC, FPLC, and Capillary Electrophoresis applications for the analysis and purification of proteins, peptides, and nucleic acids.

Bill currently is a Principal Consultant Product Manager whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of biological molecules

Speaker: Udayabagya (Bud) Halim (Sr. Product Marketing Analyst, Waters Corporation)

Bud Halimis a senior marketing analyst at Waters | Wyatt Technology with a PhD in physical chemistry from UCLA.

He began his career teaching biochemistry and chemistry and contributing to laboratory manuals before transitioning into the scientific instrumentation industry as an applications scientist.

At Waters, he supports researchers in the characterization of peptides, biologics, lipid nanoparticles (LNPs), and viral vectors.