Beyond Surface Separation: Navigating Mega Molecule Sizing with Wide Pore SEC-MALS
Better SEC Methods Begin with Understanding Pore Structure
Characterizing large biomolecules like viral vectors, plasmids, and LNPs remains a major analytical challenge due to their size, heterogeneity, and sensitivity to method conditions. Wide- and ultra-wide-pore SEC enables size-based separation, but understanding pore accessibility and true analyte behavior requires deeper insight.
In this webinar, we combine advanced SEC pore characterization with multi-angle light scattering (MALS) detection to reveal how pore structure, accessibility, and distribution impact separation performance. Learn how dual-probe approaches and SEC-MALS workflows enable accurate sizing, aggregation analysis, and robust method development for genetic medicine applications.
What you will learn:
- Understand how pore size distribution impacts SEC selectivity and resolution
- Learn differences between structural vs. chromatographic pore characterization
- Apply SEC-MALS for accurate size and molar mass of large biomolecules
- Identify optimal wide-pore SEC conditions for viral vectors, LNPs, and plasmids
- Translate pore accessibility into better method development decisions
Who should attend
Biopharma analytical development scientists, Gene therapy and Genetic medicine researchers (AAV, mRNA, LNPs), QC and CMC scientists, Chromatography and biophysical characterization specialists, Lab managers and directors in biologics and advanced therapies.
Speaker: Szabolcs Fekete PhD (Consulting Scientist, Genetic Medicine, Waters Corporation)
Szabolcs worked in the pharmaceutical industry for 10 years before joining the University of Geneva Deparment of Pharmaceutical Sciences. In 2021, he joined Waters Corporation and now works as a Consulting Scientist. He contributed to ~190 peer-reviewed journal articles and co-authored 10+ book chapters and co-edited handbooks.
His current interests include separations of new chemical modalities, fundamentals of chromatography, column technology, and method development approaches and modeling.
He was the winner of the LCGC Emerging Leader in Chromatography Award in 2020 and the HTC Innovation Award in 2022
