Enabling Robust HILIC LC–MS Workflows for Highly Polar Compound Analysis
This webinar will highlight the role of Kinetex® Amide in enabling robust and reliable HILIC LC–MS workflows for the analysis of highly polar compounds in complex matrices.
Practical application examples will include the determination of residual lactose in dietary supplements to support lactose-free claims, along with method optimization under TFA and DFA mobile phase conditions.
Attendees will gain hands-on insights into how Kinetex Amide delivers consistent retention, high selectivity, and excellent reproducibility, while preserving full LC–MS compatibility and supporting high laboratory productivity.
Key Learning Points
- Understand key considerations for HILIC LC–MS analysis of highly polar compounds.
- Learn how to improve HILIC method robustness, reproducibility, and throughput in routine analysis.
- Listen to a real case study of very polar compounds (amino acids, lactose, and organic acids).
- Find how to apply robust workflows for lactose determination in lactose‑free products.
- Review TFA and DFA determination in water.
Who Should Attend
- Any LC and LC–MS users working in food, environmental and pharmaceutical laboratories who are involved in:
- Analysis of highly polar compounds
- Verification of lactose‑free products and simple sugar
- Amino acid analysis
- TFA and DFA Analysis
- Routine QC and regulatory testing
Presenter: Luigi Margarucci (EMEA Seminar & Application Lead, Phenomenex)
Luigi, who holds a Ph.D. in Pharmaceutical Sciences, has over a decade of experience at Phenomenex. Since 2021, he has served as Technical Manager for Italy and EMEA Seminar & Application Lead. Luigi gained extensive expertise in chromatography coupled with mass spectrometry while working as a researcher at the University of Salerno and collaborating with leading centers in Italy and Europe (including Utrecht University, NL). He is the author of 27 scientific publications and has delivered hundreds of seminars on analytical techniques such as HPLC, GC, and SPE.
