Sustainable Strategies for High Sensitivity Analysis for Clinical Research LC-MS/MS Methods
One webinar, two viewing options
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Session 1
- Tuesday, June 23, 2026
- Los Angeles: 10:00 AM PT | New York: 1:00 PM EST
- Mexico City: 12:00 PM CDT
Session 2
- Wednesday, June 24, 2026
- London: 9:00 AM BST | Berlin: 10:00 AM CEST
- Mumbai: 1:30 PM IST
Supported Liquid Extraction (SLE) for the cleanup of biological matrices has several advantages over Solid Phase Extraction (SPE). SLE requires fewer steps and less method development compared to SPE. For most samples that can be processed by liquid-liquid extraction, SLE can usually be used to provide cleaner and faster solvent partitioning. Historically, to get the cleanest SLE extracts, halogenated solvents like dichloromethane were used for elution of target analytes from the sorbent. A supported liquid extraction (SLE) approach that produced clean extracts with high recoveries and low matrix interferences using ethyl acetate, a more sustainable organic solvent, will be presented. Results for steroids, methylmalonic acid (MMA), and underivatized estrogens in human serum, in addition to optimized LC-MS/MS conditions and LC column considerations, are also discussed.
Key Learning Points
- Discover how SLE provides clean extracts for LC-MS/MS quantitation at low concentrations.
- Describe conditions for the extraction and analysis of MMA from human serum.
- Define method parameters for low-level quantitation of estrogens without derivatization.
Who Should Attend
- All clinical, CROs, and forensic/toxicology analysts using LC/MS-MS.
Presenter: Stephanie J Marin (Sr. Global Market Development Manager - Applied Markets, Phenomenex)
Stephanie J. Marin is the Clinical and Forensics Global Market Development Manager at Phenomenex. She received her Ph.D. in chemistry from Arizona State University and has expertise in sample preparation, liquid chromatography, and mass spectrometry. She has over 10 years of experience developing and validating clinical methods from her tenure at the ARUP Institute for Clinical and Experimental Pathology. Dr Marin has worked in marketing, product development, and applications development, in addition to customer-facing roles in customer and technical support, service, and customer education and training. She has also held positions at Hamilton Company, Selerity Technologies, and Biotage, and in analytical services for specialty chemicals and polymers at Rohm and Haas (now Dow). She was also a supervisor at an EPA-certified laboratory. She is the author of over 30 peer-reviewed publications, book chapters, and over 100 abstracts presented at national and international meetings.
