High Throughput Native MS With Robust Ion Source Operation For The Analysis Of Proteins And Protein Complexes

Native mode proteins and protein complexes typically are analyzed using nanospray techniques or capillary LC flow rates with gentle ionization conditions to achieve best responses and preserve the native conformations. However, a few recent publications suggest that higher flow LC/MS techniques perform acceptably well for native mode protein and protein complexes.

For Research Use Only. Not for use in diagnostic procedures.

Presenter: Caroline S. Chu, PhD (LC/MS Application Scientist, Agilent Technologies, Inc.)

Caroline is an LC/MS Application Scientist has been with Agilent for 9 years, supporting the LC-Q/TOF, QQQ, and IM-QTOF MS. In addition to the mass spec platforms she also supports the automation platforms, including AssayMap and Rapid Fire. At Agilent, Caroline covers the BioPharma applications including intact proteins, native MS, glycomics, high-throughput screening and the life science applications including proteomics, targeted proteomics and metabolomics. Prior to joining Agilent she completed her Ph.D in Biophysics at the University of California, Davis, working on clinical glycomics with high resolution MS. Caroline then completed her post-doctoral work at the Institute for Systems Biology in Seattle, WA working on targeted proteomics and peptide quantitation of the human proteome for the Human SRM Atlas.