Benefits of the Cyclic IMS for HDX/MS Experiments
This large protein assembly is made up of forty-five individual subunits and Mark will discuss the application of Cyclic IMS to hydrogen deuterium exchange studies of complex I in the so called ‘active’ and ‘de-active’ conformations.
The increased ion mobility resolution and MS resolution of the cyclic is allowing Mark and his team to increase peptide coverage of the individual subunits. With improved resolution, they are able to increase the subunit complexity of the systems studied by HDX/MS.
What You Will Discover:
- HDX analysis workflow
- Expanding subunit HDX/MS complexity
- Subunit complexity of bovine mitochondrial complex I.
- Advantages of Cyclic IMS to investigate conformations
Presenter: Dr. Mark Skehel (Head of Biological Mass Spectrometry and Proteomics, Cell Biology Division, MRC Laboratory of Molecular Biology, Cambridge, UK)
Mark has been Head of Biological Mass Spectrometry and Proteomics at the MRC Laboratory of Molecular Biology since 2012. Prior to that he was Head of Protein Analysis and Proteomics at CRUK London Research Institute. He received his PhD in 1994 whilst in the lab of Sir John E. Walker FRS, working on the subunit characterisation of bovine Complex I.
