Bioanalytical method development for oligonucleotides: Overcoming the analytical challenges
In recent years, the development of next-generation oligonucleotide therapies with high target specificity and stability has greatly increased, yet the precise and productive analytical testing of these molecules has remained a significant challenge.
Trudeau will illustrate these challenges and solutions through a number of examples, including various oligodeoxythymidines and the antisense oligonucleotide therapy, GEM91, and place particular emphasis on how to achieve maximum resource utilization and improve selectivity and sensitivity in oligonucleotide bioanalysis using LC-MS/MS.
Key learning objectives:
- Gain an understanding of the analytical challenges posed by therapeutic oligonucleotides
- Learn the tips and tricks of oligonucleotide analysis
- Understand how to optimize sample prep and LC-MS acquisition for maximum sensitivity and selectivity, which are critical to the quantitative assay performance of oligonucleotides
Who should attend?
- Analytical scientists who are engaged in bioanalytical studies on oligonucleotides
- Lab managers and lab directors who run bioanalytical labs
- CROs performing bioanalytical studies on oligonucleotides
Presenter: Mary Trudeau (Principal Application Scientist/Manager-Scientific Operations Division, Waters Corporation)
Mary Trudeau is a principal applications scientist and manager within the Scientific Operations Division at Waters Corporation. Following a successful career in pharma with Pfizer, Mary joined Waters in 2011. She is responsible for developing and optimizing methods and workflows to address customer pain points and demonstrate Waters’ expertise across multiple market segments, for both small and large molecules. Her team’s primary focus has been developing and highlighting automated sample preparation workflows for both small and large molecules.
Presenter: Tom Casburn (Editorial Team, SelectScience)
Tom studied biology at the University of Bristol, UK, and as a member of the editorial team, plays an integral role in helping to shape the content on SelectScience.
