<p><strong>Flash chromatography has become one of the most widely used purification techniques in chemical and pharmaceutical laboratories. By combining speed, simplicity, and scalability, it enables researchers to isolate target compounds efficiently without the complexity and cost associated with high-pressure chromatographic systems. Applications range from synthetic chemistry and drug discovery to natural product purification and process development.</strong></p><p>The technique utilizes pre-packed columns filled with a stationary phase—most commonly silica gel—through which compounds are transported using a pressurized solvent stream. Operating pressures are significantly lower than those used in HPLC, allowing rapid separations with straightforward instrumentation. This article explores the fundamentals of flash chromatography columns, compares them with HPLC columns, discusses sample loading considerations, and highlights the advantages offered by Santai SepaFlash columns available through KNAUER.</p><h4><strong>Flash Chromatography versus HPLC</strong></h4><p>Although both flash chromatography and HPLC are based on the same chromatographic principles, their practical roles and operating conditions differ considerably.</p><h5><strong>Operating Pressure and System Design</strong></h5><p>One of the most significant differences is pressure. Flash chromatography generally operates in the low- to medium-pressure range, typically around 10–20 bar, whereas HPLC systems routinely function at several hundred bar. As a result, flash systems can utilize simpler pumps or even compressed gas sources, reducing both system complexity and operating costs.</p><h5><strong>Particle Size and Separation Efficiency</strong></h5><p>HPLC columns are packed with very small stationary-phase particles, typically 3–15 μm in diameter, which maximize chromatographic efficiency and resolution. Flash chromatography columns use larger particles, usually between 25 and 50 μm. While this reduces separation efficiency somewhat, it also lowers backpressure and permits substantially faster flow rates.</p><h5><strong>Column Construction and Dimensions</strong></h5><p>Analytical HPLC columns are typically narrow-bore stainless-steel tubes designed to withstand high pressure. Flash columns are generally wider cartridges manufactured from polypropylene or similar materials. Their larger diameter allows significantly greater sample loading, making them particularly attractive for preparative purification.</p><h5><strong>Reusability and Operating Costs</strong></h5><p>HPLC columns are intended for long-term use and require careful maintenance to preserve performance. Flash cartridges are usually pre-packed disposable consumables, allowing users to replace the column after a purification rather than performing extensive cleaning and regeneration procedures.</p><h5><strong>Typical Applications</strong></h5><p>Flash chromatography is frequently employed for routine purification of reaction products, fractionation of natural extracts, and rapid preparative separations where throughput is more important than maximum chromatographic resolution. HPLC remains the preferred option for demanding analytical measurements and high-resolution separations of structurally similar compounds.</p><h4><strong>Understanding Column Volume and Sample Loading</strong></h4><p>Successful flash chromatography depends heavily on selecting an appropriate column size and loading the correct amount of sample.</p><h5><strong>Column Volume</strong></h5><p>Column volume (CV) represents the internal volume occupied by the packed stationary phase and associated pore space. For example, a typical 4 g silica cartridge has a volume of approximately 6 mL. This value serves as an important reference for determining suitable injection volumes and operating conditions.</p><h5><strong>Injection Volume Considerations</strong></h5><p>When samples are introduced as solutions, the injection volume should remain small relative to column volume. A commonly accepted guideline is to keep injections below approximately 1–3% of the total column volume.</p><p>Excessively large injection volumes can cause:</p><ul><li>Peak broadening</li><li>Reduced resolution</li><li>Premature analyte elution</li><li>Loss of purification efficiency</li></ul><p>Consequently, highly concentrated solutions or dry-loading approaches are often preferred when larger sample quantities must be processed.</p><h5><strong>Sample Capacity</strong></h5><p>Flash columns are generally categorized according to the amount of stationary phase they contain. Loading capacity depends on separation difficulty and purity requirements.</p><p>For conventional silica columns:</p><ul><li>Easy separations may tolerate loadings approaching 10–20% of silica mass</li><li>Typical purifications are performed at approximately 5–10%</li><li>Challenging separations often require loading levels closer to 1% or less</li></ul><p>Lower loading levels generally produce superior chromatographic resolution and higher product purity.</p><h5><strong>Reversed-Phase Versus Normal-Phase Capacity</strong></h5><p>Sample capacity differs significantly between stationary-phase chemistries.</p><p>Standard silica columns provide the highest loading capacities. Reversed-phase materials such as C18 generally support only about 1–2% loading relative to media weight because a substantial portion of the silica surface is occupied by bonded ligands.</p><p>As a result, reversed-phase flash methods often require larger columns to process equivalent sample masses.</p><h5><strong>Dry Loading Strategies</strong></h5><p>Dry loading offers an effective solution when handling large sample quantities or poorly soluble materials.</p><p>In this approach:</p><ol><li>The sample is adsorbed onto a small quantity of sorbent.</li><li>The sorbent is packed into a loading cartridge.</li><li>The material is transferred onto the chromatographic column during solvent flow.</li></ol><p>Advantages include:</p><ul><li>Elimination of large solvent plugs</li><li>Improved peak shape</li><li>Higher loading capacity</li><li>Better chromatographic performance</li></ul><h4><strong>Typical Flash Column Capacities</strong></h4><p>The amount of sample that can be purified increases approximately in proportion to column size.</p><p>Examples for silica-based flash columns include:</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_Examples_of_flash_column_sizes_and_their_typical_sample_loading_capacity_70b8416017.jpg" alt="KNAUER: Table: Examples of flash column sizes and their typical sample loading capacity. Lower values in the range (~0.1% of media weight) would be used for very difficult separations requiring high purity, whereas the upper values (~10% of media weight) can be used for easier separations. These guidelines apply to normal-phase silica columns; reversed-phase columns would have much lower capacity (roughly one-tenth of the above, see text)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_Examples_of_flash_column_sizes_and_their_typical_sample_loading_capacity_70b8416017.jpg 196w," sizes="100vw" width="1152" height="916"></p><p>Actual loading capacity depends on compound complexity, required purity, and chromatographic selectivity.</p><h3><strong>Advantages of Santai SepaFlash Columns</strong></h3><p>High-quality columns can significantly improve purification performance. Santai SepaFlash columns have become well established due to their consistent packing quality, versatile design, and broad compatibility.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Sepa_Flash_a5fd81cbfd.webp" alt="KNAUER: A Santai SepaFlash pre-packed flash column (4 g size). These disposable polypropylene cartridges are filled with high-purity silica and feature Luer-lock connectors for easy setup. They are compatible with common flash chromatography systems and designed for single or few-use purification runs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Sepa_Flash_a5fd81cbfd.webp 245w," sizes="100vw" width="1022" height="234"></p><h5><strong>Ultra-Pure Silica for Reproducible Separations</strong></h5><p>SepaFlash columns utilize highly purified silica with tightly controlled particle-size distribution and minimal fine-particle content.</p><p>Benefits include:</p><ul><li>Sharp peak profiles</li><li>Reduced tailing</li><li>Excellent batch-to-batch consistency</li><li>Reliable chromatographic reproducibility</li></ul><p>The stationary phase is carefully controlled with respect to pH neutrality and moisture content, minimizing variability between production lots.</p><h5><strong>Durable Cartridge Construction</strong></h5><p>The cartridges are manufactured from pharmaceutical-grade polypropylene and supplied pre-packed and ready for immediate use.</p><p>Key advantages include:</p><ul><li>Leak-free operation</li><li>Chemical compatibility with common solvents</li><li>Elimination of manual column packing</li><li>Consistent performance from cartridge to cartridge</li></ul><h5><strong>High-Pressure Capability</strong></h5><p>Different SepaFlash product families are available to address various purification requirements.</p><p>The SepaFlash HP series supports operating pressures up to approximately 400 psi (28 bar), enabling:</p><ul><li>Higher flow rates</li><li>Improved productivity</li><li>Enhanced separation performance</li><li>More demanding preparative applications</li></ul><p>Standard flash cartridges are rated for approximately 300 psi (20 bar), providing ample capability for routine work.</p><h5><strong>Flexible Sample Introduction with iLOK Technology</strong></h5><p>The SepaFlash iLOK™ platform offers a unique approach to sample loading.</p><p>Researchers can:</p><ul><li>Perform traditional liquid injections</li><li>Utilize dry-loading techniques</li><li>Reuse loading hardware</li><li>Switch rapidly between loading approaches</li></ul><p>This flexibility simplifies workflow development and increases overall laboratory productivity.</p><h5><strong>Broad Chemistry Portfolio</strong></h5><p>The SepaFlash product family includes numerous stationary phases, including:</p><ul><li>Silica</li><li>C18</li><li>C8</li><li>Amino phases</li><li>Specialized media for cannabinoid purification</li></ul><p>This allows both normal-phase and reversed-phase purification strategies to be performed within a single product ecosystem.</p><h5><strong>Universal Instrument Compatibility</strong></h5><p>A major advantage is compatibility with virtually all leading flash chromatography platforms.</p><p>SepaFlash columns integrate seamlessly with systems from manufacturers such as:</p><ul><li>Biotage</li><li>Teledyne ISCO</li><li>Büchi</li><li>Santai</li><li>Other major flash chromatography suppliers</li></ul><p>This enables laboratories to adopt the columns without modifying existing instrumentation.</p><h5><strong>Cost-Effective Performance</strong></h5><p>Despite their premium construction and performance characteristics, SepaFlash columns remain competitively priced. Many users report chromatographic performance comparable to more expensive alternatives, making them an attractive option for laboratories seeking both quality and cost efficiency.</p><h4><strong>Conclusion</strong></h4><p>Flash chromatography remains one of the most practical and efficient purification techniques available to modern laboratories. Compared with HPLC, it provides a highly attractive balance of speed, scalability, operational simplicity, and affordability, making it ideal for preparative separations ranging from milligram to multi-gram quantities.</p><p>Achieving optimal results requires careful consideration of column size, loading capacity, and sample introduction strategy. When these factors are properly managed, flash chromatography delivers rapid and reliable purification with excellent productivity.</p><p>Santai SepaFlash columns further enhance these capabilities through high-purity stationary phases, robust cartridge construction, flexible loading options, broad instrument compatibility, and excellent reproducibility. For scientists seeking dependable purification solutions, they provide a versatile platform capable of supporting a wide range of chromatographic applications while maintaining the efficiency and reliability required in modern research laboratories.</p>

Flash Chromatography Columns: A Comprehensive Overview and Benefits of Santai SepaFlash

News from LabRulezLCMS Library - Week 22, 2026

<p><strong>Low analyte recovery is one of the most common challenges encountered during Solid Phase Extraction (SPE) sample preparation. In this Phenomenex Video Tip, Genevie Hodson, Senior Manager of Technical Support and Applications at Phenomenex, explains a practical troubleshooting approach for situations where analyte recovery after an </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=6&amp;manufacturerItems=50"><strong>SPE </strong></a><strong>procedure is lower than expected.</strong></p><p>The key objective of troubleshooting is not simply to identify that recovery is low, but to determine exactly where the analyte was lost during the extraction process. Once the point of loss has been identified, corrective actions can be implemented systematically.</p><h3><strong>Fundamental Principle of SPE Recovery Troubleshooting</strong></h3><h4><strong>Determine Where the Analyte Is Going</strong></h4><p>The most important step when troubleshooting low recovery is to identify the location of the missing analyte.</p><p>The process begins with the assumption that the sample originally contained 100% of the target analyte. If the final recovery is lower than expected, some portion of the analyte has been lost during SPE processing.</p><p>To identify the loss point, each fraction generated during the SPE procedure must be collected and analyzed separately.</p><h5><strong>Fractions to Collect</strong></h5><p>During the SPE workflow, the following fractions should be collected individually:</p><ul><li><strong>Load fraction (flow-through)</strong></li><li><strong>Wash fraction(s)</strong></li><li><strong>Elution fraction</strong></li></ul><p>Each collected fraction is transferred into a vial and analyzed using the laboratory’s analytical method.</p><p><strong>The analytical results reveal whether the analyte is:</strong></p><ul><li>Present in the load fraction</li><li>Present in one of the wash fractions</li><li>Present in the elution fraction</li><li>Missing from all collected fractions</li></ul><p>The location of the analyte provides direct information about the root cause of the recovery problem and guides the next troubleshooting steps.</p><h4><strong>Scenario 1: Analyte Detected in the Load Fraction</strong></h4><h5><strong>What It Means</strong></h5><ul><li>If the analyte appears in the load fraction, it was not retained by the SPE sorbent during sample loading.</li><li>Instead of binding to the stationary phase, the analyte passed directly through the cartridge.</li></ul><h5><strong>Possible Causes</strong></h5><ol><li><strong>Incorrect Phase Selection</strong></li></ol><ul><li>The selected SPE sorbent may not be appropriate for the analyte being extracted.</li><li>If the analyte cannot interact effectively with the sorbent chemistry, retention will not occur.</li></ul><ol start="2"><li><strong>Excessively Strong Sample Solvent</strong></li></ol><ul><li>The sample may be dissolved in a solvent that is too strong.</li><li>A strong sample solvent can prevent proper interaction between the analyte and the sorbent, causing breakthrough during loading.</li></ul><ol start="3"><li><strong>Incorrect pH Conditions</strong></li></ol><ul><li>Retention often depends on maintaining the analyte in the correct ionization state.</li><li>If the sample pH is not appropriate, the analyte may not interact correctly with the sorbent and therefore will not be retained.</li></ul><ol start="4"><li><strong>Sorbent Overload</strong></li></ol><ul><li>The SPE cartridge may have received:<ul><li>Too much analyte</li><li>Too much sample matrix</li></ul></li><li>When sorbent capacity is exceeded, analytes can break through the cartridge and appear in the load fraction.</li></ul><h5><strong>Corrective Action</strong></h5><p>When overload is suspected, increasing the amount of sorbent can improve analyte retention and prevent breakthrough.</p><h4><strong>Scenario 2: Analyte Detected in the Wash Fraction</strong></h4><h5><strong>What It Means</strong></h5><p>If the analyte appears in the wash fraction, it was initially retained but subsequently removed during the washing step.</p><h5><strong>Possible Causes</strong></h5><ol><li><strong>Wash Solvent Too Strong</strong></li></ol><ul><li>The most common cause is an overly aggressive wash solvent.</li><li>A wash solution with excessive elution strength can remove the analyte before the intended elution step.</li></ul><ol start="2"><li><strong>Incorrect pH During Washing</strong></li></ol><ul><li>When pH control is required for retention, the same conditions must be maintained throughout the process.</li><li>A pH change during washing may alter analyte retention and cause premature elution.</li></ul><h5><strong>Corrective Action</strong></h5><p><strong>Review:</strong></p><ul><li>Wash solvent composition</li><li>Wash solvent strength</li><li>pH conditions during washing</li></ul><p>Adjustments should ensure that the analyte remains retained until the elution step.</p><h4><strong>Scenario 3: Analyte Missing from All Fractions</strong></h4><h5><strong>What It Means</strong></h5><ul><li>If the analyte is not found in the load fraction, wash fractions, or elution fraction, it is likely still retained on the SPE sorbent.</li><li>In this situation, the analyte has not been effectively eluted from the cartridge.</li></ul><h5><strong>Possible Cause</strong></h5><p><strong>Elution Solvent Not Strong Enough</strong></p><ul><li>The final elution solvent may lack sufficient strength to remove the analyte from the sorbent.</li><li>As a result, the analyte remains bound to the stationary phase and never reaches the collection vial.</li></ul><h4><strong>Corrective Action</strong></h4><ul><li>Increase the strength of the elution solvent during the final SPE elution step.</li><li>A stronger solvent can improve analyte desorption and recovery.</li></ul><h3><strong>Summary of Low Recovery Troubleshooting Strategy</strong></h3><p>The recommended troubleshooting workflow consists of four steps:</p><h4><strong>1. Collect All SPE Fractions</strong></h4><p><strong>Gather:</strong></p><ul><li>Load fraction</li><li>Wash fraction(s)</li><li>Elution fraction</li></ul><h4><strong>2. Analyze Each Fraction Separately</strong></h4><p>Use the analytical method to determine where the analyte is located.</p><h4><strong>3. Identify the Root Cause</strong></h4><div><div><h5><strong>Analyte Location&nbsp;&nbsp;/&nbsp;&nbsp;Likely Cause</strong></h5><ul><li><strong>Load fraction:</strong> Incorrect phase, strong sample solvent, incorrect pH, sorbent overload</li><li><strong>Wash fraction: </strong>Wash solvent too strong, incorrect pH</li><li><strong>Not found in any fraction:</strong> Incomplete elution from sorbent</li></ul></div></div><h4><strong>4. Apply Corrective Measures</strong></h4><p><strong>Modify:</strong></p><ul><li>Sorbent selection</li><li>Sample solvent composition</li><li>pH conditions</li><li>Sorbent amount</li><li>Wash solvent strength</li><li>Elution solvent strength</li></ul><h3><strong>Other Common SPE Issues</strong></h3><p>In addition to low recovery, several other SPE-related problems are commonly encountered.</p><p><strong>These include:</strong></p><ul><li>Contamination</li><li>Reproducibility Problems</li><li>Flow-Related Issues</li></ul><h3><strong>Conclusion</strong></h3><p>Successful troubleshooting of low SPE recovery depends on identifying where the analyte is lost during the extraction process. Collecting and analyzing individual SPE fractions provides a systematic method for locating the analyte and determining the underlying cause of poor recovery.</p><p>Whether the analyte passes through during loading, elutes during washing, or remains bound to the sorbent after extraction, fraction-based investigation enables targeted corrective actions. This structured approach allows SPE methods to be optimized efficiently and helps restore acceptable analyte recovery.</p>

SPE Troubleshooting: Understanding and Resolving Low Recovery in Solid Phase Extraction

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Waters Corporation

<p><strong>As peptide-based therapeutics continue to expand, understanding and controlling aggregation behavior has become essential for ensuring drug safety and efficacy. This webinar provides a focused exploration of peptide aggregation mechanisms and how Size Exclusion Chromatography (SEC) can be applied to characterize these critical quality attributes. Attendees will be introduced to the separation principles behind SEC and learn how Biozen dSEC‑1 can improve peptide aggregate detection and quantification. Real-world applications will cover aggregation profiles of key GLP‑1 receptor agonists including semaglutide, liraglutide, and tirzepatide.</strong></p><h5><strong>Key Learning Points</strong></h5><ul><li>Learn about the fundamentals of peptide aggregation</li><li>Find the separation mechanism of Size Exclusion Chromatography (SEC)</li><li>Identify the key features and advantages of the Biozen dSEC‑1 column</li><li>Discuss the use of Biozen dSEC-1 for aggregate analysis of GLP-1 agonists (Semaglutide, Liraglutide, and Tirzepatide)</li></ul><h5><strong>Who Should Attend</strong></h5><ul><li>Any LC users working with or interested in analyzing peptide therapeutics by HPLC. &nbsp;</li></ul><blockquote><p><strong>Speaker:</strong> Lucia Geis Asteggiante (Senior Technical Specialist, Phenomenex)</p><p>Lucia is a Senior Technical Specialist that has recently joined Phenomenex’s Global Technical Team. She has a broad experience in the field of Analytical Chemistry with a particular focus on LC chromatography coupled with mass spectrometry. Lucia comes from a strong academic and regulatory background with experiences ranging from method development and analysis of small molecules to large protein complexes. She gained her Ph.D. at the University of Maryland - College Park, USA and did her post-doctoral work at the University of Oxford, UK. During her time as a researcher, she has authored/co-authored 23 scientific publications and 2 book chapters.</p></blockquote>

Phenomenex: Reliable GLP-1 Aggregate Analysis Using Biozen dSEC

Reliable GLP-1 Aggregate Analysis Using Biozen dSEC

This webinar provides an exploration of peptide aggregation mechanisms and how SEC can be applied to characterize these critical quality attributes.

<p><strong>PFAS, often referred to as “forever chemicals,” are a diverse class of anthropogenic compounds that have become a major public health concern. Their wide range of structures and frequent presence in laboratory materials create unique analytical challenges. These challenges are especially pronounced for ultra‑short‑chain PFAS, whose high polarity, limited retention on conventional reversed‑phase columns, and tendency to coelute with matrix components make them particularly difficult to separate and quantify.&nbsp;</strong></p><p>In this webinar, we will discuss key considerations for selecting an LC separation technique and choosing appropriate consumables—along with strategies to address the chromatographic complexities of ultra‑short‑chain PFAS—to help streamline PFAS analysis.</p><blockquote><p><strong>Speaker: </strong>Emily Parry, Ph.D.</p><p>Emily Parry holds a Ph.D. in Environmental Chemistry from the University of California, Davis, where she studied how wastewater treatment transforms environmental contaminants. After completing her degree, she began her career conducting research at the Environmental Protection Agency (EPA) and the Public Health Institute. In 2018, she joined Agilent Technologies as an application scientist focused on small‑molecule analysis using LC‑QTOF and LC‑TQ, specializing in environmental applications. In 2026, she transitioned to Agilent’s LC columns group, where she supports customers and applications in small‑molecule separations. Over the years, she has developed deep expertise in analytical methods for measuring small molecules across diverse sample types.</p></blockquote>

Agilent Technologies: Navigating PFAS Complexity: Addressing the Unique Chromatographic Challenges of Ultra‑Short‑Chain PFAS

Navigating PFAS Complexity: Addressing the Unique Chromatographic Challenges of Ultra‑Short‑Chain PFAS

Key considerations for selecting an LC separation technique and choosing appropriate consumables.

Chromatographic Challenges of Ultra‑Short‑Chain PFAS

<p><strong>Environmental labs are facing increasing pressure to address ultrashort-chain PFAS as regulatory scrutiny accelerates and begins to influence current testing priorities. Many still lack clarity on when these compounds matter, how evolving EPA direction and European regulations apply, and how to manage analytical trade-offs.</strong></p><p>This webinar explores real-world measurement challenges and introduces a practical decision framework to support confident, fit-for-purpose strategies. Attendees will gain insight into aligning methods with specific analytical goals, improving reliability while avoiding one-size-fits-all workflows.</p><h4><strong>Attend this webinar to:</strong></h4><ul><li>Understand why ultrashort-chain PFAS are becoming increasingly relevant in environmental analysis</li><li>Recognize how EPA direction and current European regulations may influence USC PFAS testing priorities</li><li>Identify the main analytical and workflow challenges in measuring USC PFAS with confidence</li><li>Apply a practical decision framework to distinguish between screening, quantitation, and compliance-driven needs</li></ul><blockquote><p><strong>Speaker: </strong>Ivan Huang (Small Molecules Analytical Columns Product Manager, Agilent Technologies)</p></blockquote>

Technology Networks: Ultrashort-Chain PFAS: Practical Guidance for Testing Labs

Ultrashort-Chain PFAS: Practical Guidance for Testing Labs

Understand why ultrashort-chain PFAS are becoming increasingly relevant in environmental analysis

<h4><strong>Reduce Measurement Errors and Improve Confidence in pH Results</strong></h4><p><strong>This webinar covers practical approaches to improving pH measurement accuracy in environmental testing, with a focus on avoiding common errors that lead to proficiency testing failures.</strong></p><p>pH is one of the most commonly measured - and most frequently failed -parameters in environmental testing. With tight proficiency testing acceptance limits and strong temperature dependence, even small measurement errors can lead to unacceptable results. This webinar provides a practical, end to end overview of pH measurement, with a focus on improving confidence, consistency, and proficiency testing performance.</p><p>Throughout the presentation, we connect theory to practice with actionable tips that can be applied immediately in the laboratory. Whether you are troubleshooting a recent pH failure or looking to strengthen routine measurement practices, this webinar is designed to help laboratories “get pH right” and improve long term measurement reliability</p><h4><strong>What you will learn:</strong></h4><ul><li>Understand pH fundamentals and regulatory proficiency testing requirements.</li><li>Apply best practices for accurate pH measurement, calibration, and quality control.</li><li>Maintain and troubleshoot pH probes to ensure reliable performance.</li></ul><h4><strong>Who should attend</strong></h4><ul><li>Analytical scientists</li><li>Laboratory managers</li><li>Quality assurance managers</li></ul><blockquote><p><strong>Speaker:&nbsp;</strong>Christy Abbas, PhD (Sr. Product Marketing Manager, Waters ERA)</p><p>Christy has been with Waters ERA for six and a half years. Her career spans roles at an instrument manufacturer, a biopharmaceutical laboratory, and a mining company. She earned her PhD in Applied Chemistry from the Colorado School of Mines.</p><p>Throughout her career, Christy has supported the nuclear, pharmaceutical, and chemical industries, as well as academia. Drawing on her background in analytical chemistry, product development, marketing, and sales, she is focused on expanding Waters ERA’s product portfolio to better meet evolving customer needs.</p></blockquote>

Getting pH Right

Understand pH fundamentals and regulatory proficiency testing requirements.

<p><strong>Get an exclusive pre-ASMS 2026 sneak peek into how Evosep is driving the next phase of proteomics standardization. Building on our Open Innovation Initiative, this webinar will showcase upcoming advancements in Evotip-based sample preparation, harmonized protocols, and integrated workflows designed for scalability and reproducibility -addressing one of proteomics’ biggest challenges: sample preparation.</strong></p><blockquote><p>Speakers to be announced</p></blockquote>

Evosep: Preparing for Next Generation Evosep Proteomics

Preparing for Next Generation Evosep Proteomics

Join Evosep’s pre-ASMS 2026 webinar on proteomics standardization. Explore Evotip advancements and scalable workflows for reproducible sample preparation.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Standard microbiological screening only detects viable bacteria, leaving heat-stable emetic toxins unquantified. Cereulide survives sterilization and remains in food long after Bacillus cereus has been destroyed. This surveillance gap became visible during the 2025–2026 infant formula recalls, when contaminated arachidonic acid (ARA) oil passed standard assays simply because no living organism was present.</strong></p><p>This webinar details an LC-MS/MS protocol developed specifically for these dietary ingredients, with repeatability below 9% and spike recoveries from 87% to 103%. It establishes the analytical confidence needed for proactive risk assessment of specialty ingredients historically absent from routine monitoring.</p><h5><strong>By attending this webinar, you will:</strong></h5><ul><li>See how an LC-MS/MS method reaches a 0.05 µg/kg limit of quantitation for cereulide across infant formula, ARA oil, and dairy products</li><li>Examine the validation parameters that confirm the method holds across powders, oils, and emulsions where conventional sample prep breaks down</li><li>Understand how the method aligns with EFSA recommendations and the established Acute Reference Dose for cereulide</li></ul><h5><strong>Who should attend?</strong></h5><ul><li>This webinar is ideal for food safety and quality assurance professionals, analytical chemists, and microbiologists working in the food, dairy, and infant nutrition industries.</li></ul><blockquote><p><strong>Presenter: </strong>Dr. Ming Gao (Senior Research Chemist at Mérieux NutriSciences)</p><p>Dr. Ming Gao is a Senior Research Chemist at Mérieux NutriSciences, where he has been a key member of the R&amp;D team (now RDI) in Crete, IL since 2015. His expertise lies in method development and validation using LC-MS, HPLC, GC, and GC-MS platforms. His recent achievements include the development of advanced analytical methods for detecting emetic toxins and PFAS via LC-MS/MS, carotene and asparagine via HPLC, and ethylene/propylene oxide via GC-MS/MS. Dr. Gao has been an active member of AOAC International since April 2016 and joined the AOAC PFAS Working Group in December 2022. He currently leads several projects, ensuring their efficient and timely execution. To date, Dr. Gao has authored one book chapter, one patent, and 19 peer-reviewed journal articles, with publications in JACS, PNAS, and JAOAC.</p></blockquote></div></div></div>

SeparationScience: Connecting Microbiology and Toxicology: LC-MS/MS Methods for Cereulide Quantitation in Food Matrices

Connecting Microbiology and Toxicology: LC-MS/MS Methods for Cereulide Quantitation in Food Matrices

Discover LC-MS/MS method for cereulide analysis in infant formula, ARA oil, and dairy products.

LC-MS/MS for Cereulide Quantitation in Food Matrices

<div><p><strong>In this live webinar, Rakesh (USP) and Bretton (Waters) bring together regulatory insight and practical analytical expertise to explore strategies to assess critical quality attributes (CQAs).</strong></p><p>Rakesh will focus on the analysis of comparative analytical assessment studies from successful regulatory filings of mAb biosimilars in the U.S. and EU, which identified the most common CQAs and analytical methods included in comparative studies and highlight increased adoption of more specific and efficient "analytical methods."</p><p>Biosimilarity assessment requires an extensive set of analytical tools that directly address the biophysical characterization parameters emphasized in the latest U.S. FDA biosimilar guidance and ICH Q6B . Size exclusion chromatography combined with multi-angle light scattering (SEC-MALS) has emerged as a critical technique for defining molecular weight, aggregation, impurity profiles, and related quality characteristics that support regulatory decision-making processes.</p><p>Bretton will explain how SEC-MALS can be used to measure these characteristics for biosimilars and other biological modalities.</p><blockquote><p><strong>Presenter:</strong> Rakesh Aithal, Ph.D. (Team Lead, Senior Scientist I, Pipeline Development, Global Biologics, United States Pharmacopeia)</p><p>Rakesh Aithal is currently working as a Team Lead, Senior Scientist I in the Pipeline Development Team of Global Biologics at USP. In this role, he works closely with scientific experts and stakeholders to develop physical and documentary standards used to support development and quality assessment of biopharmaceuticals with a major focus on protein therapeutics including monoclonal antibodies and antibody conjugates. He did his Ph.D. in Biomolecular Chemistry from Masaryk University and worked as a post-doctoral researcher in Cancer Research Center Marseille on the mechanistic understanding of meiotic recombination before transitioning into the biopharmaceutical industry. He has extensive expertise in a range of analytical methods for protein characterization and molecular biology techniques; and has published several research articles in peer reviewed journals. His expertise includes analytical method development, qualification, and validation along with comparative analytical similarity assessments to support biosimilar development.</p><p><strong>Presenter: </strong>Bretton Fletcher, Ph.D. (Scientist, Waters | Wyatt Technology)</p><p>Bretton Fletcher, Ph.D. is an application scientist in the Analytical Sciences team at Waters | Wyatt Technology. He assists customers in identifying the best light scattering solutions for their analytical needs by leveraging her expertise in multi-angle light scattering. He performs feasibility studies to characterize customer samples and participates in Wyatt’s Light Scattering University. He completed his Ph.D. in Biomolecular Sciences and Engineering from the University of California, Santa Barbara.</p><p><strong>Moderated by: </strong>Samuel Seal (Biopharma Market Development Manager, Waters Corporation)</p><p>Samuel Seal is a market development manager specializing in the biologics space. He loves LC and MS – especially on how this technology provides benefit and value.</p><p>Sam joined Waters in January of 2014 as a Sales Technical Support Specialist in the Irvine office/lab. As a TSS he performed demonstrations on various instruments, provided training for customers, often presented on technical information at varying events, and assisted with troubleshooting from Colorado to Los Angeles.</p><p>He previously worked at Pyxant Labs in Colorado Springs, CO where he gained extensive experience developing and validating methods for small/large molecules in bioanalytical assays, agricultural commodities, registration of pesticides, and worker exposure studies. He also has experience in column chromatography, sample extraction, sample clean-up, reporting and data management. Sam has hands on experience with running and maintaining various manufacturers' LC/MS/MS systems. Prior to Pyxant, Sam was a Laboratory Instructor at the University of Colorado at Colorado Springs where he earned his Bachelor of Arts in Biology and Bachelor of Arts in Chemistry.</p></blockquote></div>

Is your Biosimilar, Similar?

Learn how SEC-MALS supports biosimilarity assessment of mAbs through CQA evaluation, aggregation analysis, and regulatory-aligned analytical characterization.

<p><strong>Discover how IC-MS is transforming disinfection by-products analysis and helping laboratories stay ahead of evolving regulatory demands.&nbsp;</strong></p><blockquote><p><strong>Presenter:</strong> Wai-chi Man (Product Marketing Manager, Thermo Fisher Scientific)</p><p>Wai-Chi has specialized in ion chromatography since 1989 and has been with Thermo Fisher Scientific since 2011, supporting a wide range of customers across multiple industries. She providesexpertisein ion chromatography (IC), including method development, troubleshooting, sample preparation, training, andseminar, event, and workshop delivery.</p><p>Over the course of her career, she has developed extensive knowledge and experience in IC-MS, IC-ICP-MS, carbohydrate analysis, metabolomics, and polar pesticide analysis.</p><p>Beforejoining Thermo Fisher Scientific, Wai-Chi worked atWellcomein research and later at GlaxoSmithKline, where she was involved in drug discovery and developmentthrough tomanufacturing.</p><p>She is passionate about promoting STEM to the next generation of scientists and is committed to encouraging, nurturing, and supporting future talent in science.</p></blockquote>

Thermo Scientific: Modern approach to analyze disinfection by-products in water 

Modern approach to analyze disinfection by-products in water 

Discover how IC-MS is transforming disinfection by-products analysis, helping your laboratory stay ahead of evolving regulatory demands 

Modern approach to analyze disinfection by-products in water

<h3><strong>Bringing laboratory automation to life with Andrew+ and OneLab</strong></h3><p style="color:rgb(0, 0, 0);font-family:&quot;Segoe UI&quot;, &quot;Segoe UI&quot;, SegoeUI, Frutiger, &quot;Frutiger Linotype&quot;, &quot;Dejavu Sans&quot;, &quot;Helvetica Neue&quot;, Arial, sans-serif;font-size:14px;font-style:normal;font-weight:400;letter-spacing:normal;line-height:initial;text-align:start;text-indent:0px;text-transform:none;">This webinar series explores how Andrew+ automated liquid handling and OneLab workflow software support consistent, traceable and efficient laboratory operations. Through application‑focused episodes, you will see how automation can be successfully integrated into everyday laboratory workflows, from method development to routine and regulated environments, supported by customer experiences and testimonials.</p><p><span style="color:#212121;"><strong>Episode 3 – Pharma Workflows</strong></span></p><h4><span style="color:#212121;"><strong>Overview</strong></span></h4><p>Pharmaceutical laboratories require efficient, consistent and compliant workflows. In this episode, we show how automation can be implemented across multistep sample preparation processes to reduce variability and improve laboratory efficiency, supported by <strong>customer experiences from pharmaceutical environments.</strong></p><h4><span style="color:#212121;"><strong>Key highlights</strong></span></h4><ul><li>Reduce analyst-to-analyst variability in pharmaceutical sample preparation</li><li>Automate workflows ranging from basic liquid handling to filtration, extraction and magnetic bead protocols</li><li>Support data integrity and compliance through centralised workflow management</li></ul><blockquote><h4><span style="color:#212121;"><strong>Speakers</strong></span></h4><p><strong>Tobias Langrock</strong> (Senior Application Specialist Chemistry, Europe - Waters Corporation)</p><p>During his time as a PhD student Tobias discovered his passion for LC and LC-MS/MS and has not lost it since. Joining Waters in 2008 was the perfect opportunity to expand his understanding and expertise in chromatography and mass spectrometry. Since then, he has been involved with a very wide range of instruments and applications, whether small or large molecules, reversed phase LC, IEX or SEC, SFC or GPC. His focus is always on the customers and their daily chromatographic challenges.</p><p><strong>Customer testimonials: Regeneron Ireland</strong></p><p>Regeneron Ireland is part of a global pharmaceutical company focused on the discovery, development and manufacture of innovative medicines.</p><p>In this episode, the company shares its experience implementing automated liquid handling to support multistep pharmaceutical sample preparation workflows. The testimonial highlights how automation helps reduce analyst‑to‑analyst variability, minimise manual input and improve overall laboratory efficiency in a process development environment.</p></blockquote><h5><strong>Reserve Your Spot Today!</strong></h5>

Transform Your Daily Lab Operations with Automation - Pharma Workflows

Waters webinar on automated pharmaceutical workflows, sample prep efficiency, and reduced analyst-to-analyst variability.

Transform Daily Lab Operations with Automation - Pharma

<h3><strong>Getting Data In and Out of Empower Software - Basic Steps&nbsp;</strong></h3><p>In this Coffee Break session, we’ll explore how to create and work with export methods in Empower Software.&nbsp;</p><p>There are many situations where raw data, results, or reports need to be exported from Empower to other applications such as transferring information into a LIMS.&nbsp;</p><p>Export Methods provide a straightforward and reliable way to support these data exchange needs.&nbsp;</p><h4><strong>What you’ll learn:&nbsp;</strong></h4><ul><li>Core concepts of Export Methods&nbsp;</li><li>How to export raw data, results, and reports&nbsp;</li><li>Secure transfer of Empower data into NuGenesis for Excel workflows&nbsp;</li></ul><p>This session is ideal for users looking to understand the essentials of data export from Empower in a practical, easy to apply way.&nbsp;</p><p>Prepare a coffee and attend!</p><blockquote><p><strong>Presenter: </strong>Ralf Schröder (Informatics Market Development Manager)</p><p><strong>Presenter:&nbsp;</strong>Carsten Fischer (Informatics Product Marketing, EMEA)</p></blockquote>

Waters Corporation: Informatics series #32 Empower Data Exchange - Core Features

Informatics series #32 Empower Data Exchange - Core Features

Learn the essentials of data export from Empower Software. Discover how to create export methods for secure data transfer into LIMS and NuGenesis workflows

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