Struggling to accurately measure semi-volatile analytes? Stricter temperature control with HPLC-CAD analysis just might be the solution you need.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_1_f2eefd5901.jpeg" alt="Thermo Fisher Scientific How to Get More Accurate HPLC Quantitation of Semi-Volatiles" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_1_f2eefd5901.jpeg 245w," sizes="100vw" width="1024" height="512">The charged aerosol detector (CAD) is cited as one of the most capable near-universal HPLC detectors available because the response is solely based on particle charge and fully independent of chemical structure.One caveat to know is your mobile phase components must be less volatile than the analytes in solution for detection by the CAD. And while all non-volatiles give a similar uniform response, <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/liquid-chromatography-information/hplc-system-components/how-hplc-detectors-work/charged-aerosol-detection/charged-aerosol-detection-cad-performance-tips.html">the response of semi-volatiles</a> depends on volatility and consequently is less predictable.So, the question then becomes is there a way to address this performance difference and make semi-volatile detection more predictable? One approach for charged semi-volatile analytes is to add a volatile ion-pairing agent of the opposite charge in the mobile phase to force non-volatile behavior.However, this method is analyte specific and may also react with impurities in your mobile phase, leading to higher noise and poorer signal-to-noise (S/N) ratio. What’s really needed is a more global approach to improve the accuracy for measuring semi-volatiles.This approach is now achievable with Temperature Coupling Mode (TCM) in the <a target="_blank" rel="noopener noreferrer" href="https://discover-pharma.com/thermo-fisher-upgrades-charged-aerosol-detection-tech-to-improve-complex-drug-analysis/">Thermo Scientific Vanquish CAD P Series</a>.<h3>Why stricter temperature control matters for measuring semi-volatiles</h3>Before we get into the specifics, you need to understand why temperature control in the CAD is critical to quantitative accuracy, especially for semi-volatiles (Figure 1).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_2_e33cff4cac.jpeg" alt="Thermo Fisher Scientific: How to Get More Accurate HPLC Quantitation of Semi-Volatiles: Figure 1. Illustration showing how the CAD works." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_2_e33cff4cac.jpeg 245w," sizes="100vw" width="1024" height="512">The two <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/liquid-chromatography-information/hplc-system-components/how-hplc-detectors-work/charged-aerosol-detection.html">most important functional components in the CAD that guide sensitivity</a> are the evaporation tube temperature (EvapT) and the charging detection module (CDM).<ul><li>EvapT plays a crucial role in determining the performance of the detector, and changing this temperature may alter analyte “selectivity”</li><li>The CDM is where particle charge and measurement happen</li></ul>In previous CAD models, the EvapT is adjustable, but the temperature of the CDM is permanently set at 40 °C. This fixed temperature difference between the EvapT and CDM could negatively impact the measurement of semi-volatile compounds that require a lower EvapT.Adding TCM resolves this issue, by linking the EvapT and CDM temperatures, thereby improving the quantitation of semi-volatile species.<h3>Using TCM to help improve quantification of semi-volatiles, like fatty acids</h3>To demonstrate the effectiveness of this new temperature coupling feature, we anlazyed a working solution of four individual fatty acids (FAs) and compared the CAD repsonse with TCM turned on and turned off (Figure 2).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_3_da6cf5006c.jpeg" alt="Thermo Fisher Scientific: How to Get More Accurate HPLC Quantitation of Semi-Volatiles: Figure 2. The mean S/N ratio of the FAs analyzed with TCM turned on and off." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_3_da6cf5006c.jpeg 245w," sizes="100vw" width="700" height="410">Notably, we observed 46.8% increase in the S/N at the optimal EvapT of 25 °C and with the TCM switched on for lauric acid, the most semi-volatile compound!Interestingly, the observed S/N improvement matches the respective analyte volatility—lauric acid (lowest MW) shows the greatest improvement, and stearic acid (highest MW) the least.Taken together, these findings show how stricter temperature control in the CAD can allow you to extend the range and improve quantification of semi-volatiles.<h3>Extending this application to impurity analysis of polysorbates</h3>Now that we’ve seen TCM is useful for analyzing fatty acids, let’s consider more complex solutions, like polysorbates.Given the CAD is well-established for determining <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0021967324010240?via%3Dihub">polysorbate impurities</a>, and fatty acid impurities are quite abundant in polysorbates, and a logical next step is to use this technique for analyzing these solutions.Afterall, free acids in polysorbates <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/blog/analyteguru/review-of-analytical-methods-for-quantifying-amp-profiling/">may form insoluble particles</a> that can seriously compromise product quality, efficacy, and patient safety. Thus, your ability to accurately measure these acids in raw materials and finished goods is of the upmost importance.Download our paper to see how the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_003867_hplc_vanquish_cad_gradient_optimized_evap_temp_an003867_na_en_b64411eb47.pdf">Vanquish CAD P Series</a> can help your lab measure fatty acids with greater confidence today.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_4_70473e3696.jpeg" alt="Thermo Fisher Scientific: How to Get More Accurate HPLC Quantitation of Semi-Volatiles: Figure 3. Thermo Scientific Vanquish CAD P Series" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Get_More_Accurate_HPLC_Quantitation_of_Semi_Volatiles_4_70473e3696.jpeg 245w," sizes="100vw" width="700" height="410"><h3>FAQs</h3><ol><li>What are the advantages of using a charged aerosol detector for HPLC analysis?</li></ol>The <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/c/b/vanquish-charged-aerosol-detector-p-series.html">charged aerosol detector</a> (CAD) is now recognized as the most capable “universal” detector available and offers several advantages over other UHPLC-based detectors including:<ul><li>Uniform response for all non-volatile analytes (ideal for quantitation when certified standards are unavailable)</li><li>Universal response independent of chemical structure (analyte does not have to form gas phase ions or possess a chromophore)</li><li>High sensitivity at high pg to low ng on column (ideal for drug impurity analysis)</li><li>Wide linear and dynamic range (measure API and impurities in one run)</li><li>Simple calibration curves (no need to deal with complex, unpredictable sigmoidal calibration curves)</li><li>Gradient compatible (inverse gradient available to ensure consistent response throughout the gradient)</li></ul><ol start="2"><li>Why should I use CAD for measuring fatty acid impurities in polysorbates?</li></ol>Fatty acids don’t contain strong chromophores, so HPLC-CAD analysis is the <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/blog/analyteguru/cad-vs-elsd-which-hplc-detector-is-your-better-option/">ideal choice</a> since the detector response depends on particle charge and is independent of analyte structure.<ol start="3"><li>How does changing the evaporation tube temperature affect the CAD response for semi-volatiles?</li></ol>A high EvapT can <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_72806_uhplc_charged_aerosol_detection_tn72806_en_5979117651/tn-72806-uhplc-charged-aerosol-detection-tn72806-en.pdf">improve sensitivity</a> for non-volatiles by reducing noise but may negatively impact measurement of semi-volatile species. Conversely, a lower EvapT can improve the detection of semi-volatile species, but sensitivity for all analytes may be adversely impacted due to increased noise.Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#HPLC #CAD #biopharma<section><blockquote><h5>Dr. Kimberly M. Lincoln</h5>Dr. Kimberly M. Lincoln is a marketing specialist in the HPLC division at Thermo Fisher Scientific. She holds a Ph.D. in bioinorganic chemistry and a B.S. in chemistry, with professional experience in digital marketing, medical device manufacturing, and chemical synthesis. Kimberly enjoys writing educational chemical content as a guide to help customers solve analytical problems. She is passionate about living a clean lifestyle and keeping the planet healthy for future generations. In her spare time, Kimberly loves to cook, cycle, attend rock concerts, and spend time with her pit bulls.</blockquote></section>

How to Get More Accurate HPLC Quantitation of Semi-Volatiles

We are pleased to announce that a maintenance release is now available for KnowItAll 2026. Maintenance releases are an important way to introduce improvements and bug fixes between major releases to make sure users get the most out of KnowItAll.In Fall 2025, we announced the KnowItAll 2026 major release featuring a new principal component analysis tool, Trendfinder, and various advances for spectral analysis workflows. This is the first update available after that release.<h5>Updates in the 2026.1 maintenance release include:</h5><ul><li>Continued to LC-MS deconvolution improvement</li><li>Updated the DEA regulations rulebase that’s used to automatically categorize structures in databases you build in MineIt according to current Drug Enforcement Agency controlled substance regulations.</li><li>Various bug fixes</li></ul>We encourage users to update now to the most recent version of the software to ensure the best performance.<ul><li><a target="_blank" rel="noopener noreferrer" href="https://get.knowitall.com/Download?_gl=1*18h0vjr*_gcl_au*Mjk3ODY0Mzg1LjE3MTMyNzcyMDEuMTkwNDkyNDI4NS4xNzEzMzY3NDc0LjE3MTMzNjc0Nzk.">Download the most recent version here</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/whats-new-in-knowitall-2026/">What's New in KnowItAll 2026</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/knowitall-version-history/">KnowItAll version history</a></li></ul><h4>About KnowItAll</h4>KnowItAll Software offers solutions to analyze and manage multiple types of spectral and chemical data in multiple file and instrument formats. KnowItAll's integrated toolsets eliminate the need for multiple software packages and increase overall lab efficiency and staff proficiency. Combined with Wiley’s comprehensive, high-quality spectral reference libraries, it provides an unparalleled solution for fast, reliable spectral analysis. Learn more.<h4>About <a target="_blank" rel="noopener noreferrer" href="https://www.wiley.com/en-ie/">Wiley</a></h4>Wiley (NYSE: WLY) is a global leader in authoritative content and research intelligence for the advancement of scientific discovery, innovation, and learning. With more than 200 years at the center of the scholarly ecosystem, Wiley combines trusted publishing heritage with AI-powered platforms to transform how knowledge is discovered, accessed, and applied. From individual researchers and students to Fortune 500 R&amp;D teams, Wiley enables the transformation of scientific breakthroughs into real-world impact. From knowledge to impact—Wiley is redefining what's possible in science and learning. Visit us at Wiley.com and Investors.Wiley.com. Follow us on Facebook, X, LinkedIn and Instagram.

KnowItAll 2026.1 maintenance release now available - Upgrade for the latest features and fixes

<h3>Introduction</h3>Modern analytical laboratories often operate multiple chromatographic instruments simultaneously, which places high demands on software usability and workspace navigation. <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/999">Clarity Chromatography Software</a> developed by DataApex provides a structured, instrument-oriented user interface designed to simplify work even in complex multi-instrument environments. This article explains how to efficiently move between windows in Clarity.<h3>Concept of Instrument-Centric Workspace Organization</h3><h4>Instrument-Specific Color Schemes</h4>Each instrument in Clarity is visually distinguished by its own color scheme. This design choice allows users to:<ul><li>Instantly recognize which windows belong to the same instrument</li><li>Avoid confusion when multiple instruments are open simultaneously</li><li>Maintain orientation within complex analytical workflows</li></ul>Color coding is consistent across all windows associated with a given instrument.<h3>Types of Windows in Clarity</h3>Within a single instrument context, Clarity typically provides access to several interconnected windows, including:<ul><li>Instrument Window – the central control and overview window</li><li>Chromatogram Window – displays acquired chromatographic data</li><li>Sequence Window – manages sample sequences and runs</li></ul>Efficient navigation between these windows is essential for smooth operation.<h3>Navigation Methods Between Windows</h3>Clarity offers multiple complementary ways to switch between related windows. Users can choose the method that best fits their workflow preferences.<h4>1. Instrument Window Tiles</h4>Within the Instrument Window, large tiles represent related windows.Example workflow:<ul><li>Select the large chromatogram tile to switch directly from the Instrument Window to the Chromatogram Window</li></ul>This tile-based approach is intuitive and visually guided.<h4>2. Toolbar Icons</h4>Each instrument window contains a toolbar with window icons that provide direct access to other related windows.Key characteristics:<ul><li>The same set of window icons is available across all instrument-related windows</li><li>Enables quick transitions, for example:<ul><li>From Chromatogram Window → Sequence Window</li></ul></li><li>Ideal for experienced users who prefer icon-based navigation</li></ul><h4>3. Window Menu</h4>For users who prefer menu-driven control, Clarity includes a Window menu.Typical use case:<ul><li>Select Main from the Window menu to return to the Clarity main window</li></ul>This method is particularly useful when:<ul><li>Navigating back to the global workspace</li><li>Managing multiple instruments at once</li></ul><h3>Practical Navigation Workflow Example</h3>A typical navigation sequence demonstrated in the video follows this logic:<ol><li>Start in the Instrument Window</li><li>Click the Chromatogram tile to open chromatographic data</li><li>Use the toolbar icon to switch to the Sequence Window</li><li>Open the Window menu and select Main to return to the Clarity main interface</li></ol>This combination of tiles, icons, and menus ensures flexible and efficient workspace control.<h3>Advantages of Clarity’s Navigation Design</h3>The navigation system in Clarity provides several practical benefits:<ul><li>Consistency – identical navigation tools across instrument windows</li><li>Efficiency – rapid switching without losing context</li><li>Scalability – effective even with many instruments open</li><li>User preference flexibility – toolbar or menu-based workflows</li></ul>These features are especially valuable in high-throughput laboratories and 24/7 operational environments.<h3>Conclusion</h3>Clarity Chromatography Software uses a well-structured, instrument-oriented window system that simplifies navigation even in complex multi-instrument setups. By combining color-coded instruments, tile-based access, universal toolbar icons, and traditional menus, Clarity allows users to move quickly and confidently between windows.Mastering these navigation techniques significantly improves workflow efficiency, reduces errors, and enhances overall user experience—making Clarity a robust platform for modern chromatographic laboratories.For additional tutorials and practical demonstrations, users are encouraged to explore further educational resources provided by DataApex.

How to switch between the Clarity Windows

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Separation Science

Agilent Technologies

Phenomenex

Shimadzu Corporation

SCIEX

Evosep Biosystems

SciY/Mestrelab Research

<h3>Key Learning Points</h3><ul><li>Understand the importance of knowing your analyte and how they influence analysis and optimization</li><li>Learn the importance of an analyte’s physicochemical properties</li><li>Gain an understanding of the main chemical interactions during analyte separation</li></ul><h5>Who Should Attend</h5>Anyone interested in better understanding the role of an analyte’s physicochemical properties and how these drive chromatographic separations.<blockquote>Presenter: Lucia Geis Asteggiante (Senior Technical Specialist, Phenomenex)Lucia is a Senior Technical Specialist that has recently joined Phenomenex’s Global Technical Team. She has a broad experience in the field of Analytical Chemistry with a particular focus on LC chromatography coupled with mass spectrometry. Lucia comes from a strong academic and regulatory background with experiences ranging from method development and analysis of small molecules to large protein complexes. She gained her Ph.D. at the University of Maryland - College Park, USA and did her post-doctoral work at the University of Oxford, UK. During her time as a researcher, she has authored/co-authored 23 scientific publications and 2 book chapters.</blockquote>

Phenomenex: Knowing your Analyte Properties to Optimize Analysis

Knowing your Analyte Properties to Optimize Analysis

Understand the importance of knowing your analyte and how they influence analysis and optimization

Long pathlength cuvettes are critical options for improving UV-VIS sensitivity. With pathlengths greater than traditional 1 cm, you can improve concentration analysis detection limits.The Cary 3500's flexible sampling options make this concentration analysis more effortless than ever before.<blockquote>Presenter: Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.</blockquote>

Agilent Technologies: Effortless UV-VIS Analysis with Long Pathlength Measurements

Effortless UV-VIS Analysis with Long Pathlength Measurements

The Cary 3500's flexible sampling options make this concentration analysis more effortless than ever before.

UV-VIS Analysis with Long Pathlength Measurements

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>Working with liquid chromatography (LC) brings the constant challenge to deliver clean separations and defensible data. Labs face column variability, drifting retention times, pressure spikes, ion-pairing issues, and the ongoing challenge of designing methods that hold up across instruments, sites, and sample types.Join us for our next 'Expert Answers' session in which Jim Grinias, Professor of Chemistry at Rowan University, will tackle your LC questions. Whether you work in research, QC, or regulated environments, this session gives you space to raise the topics that matter most to your lab.<h5>By attending this webinar, you will learn:</h5><ul><li>Practical ways to think through LC troubleshooting</li><li>Factors that influence LC performance across instruments and workflows</li><li>Guidance to strengthen everyday method decisions</li><li>Resources that help elevate routine LC work</li></ul><blockquote>Presenter: Dr. James Grinias (Professor, Department of Chemistry &amp; Biochemistry, Rowan University)James Grinias is a Professor in the Department of Chemistry &amp; Biochemistry at Rowan University in Glassboro, New Jersey, USA. Dr. Grinias has a number of research interests focused on chemical separations and microfluidics, both at the fundamental level and for the analysis of biological systems. He is the recipient of the 2021 American Chemical Society Satinder Ahuja Award for Young Investigators in Separation Science and the 2022 LCGC Emerging Leader in Chromatography. At Rowan, he teaches courses in general, analytical, and bioanalytical chemistry while also conducting research on UHPLC column performance and instrument miniaturization.</blockquote></div></div></div>

SeparationScience: Expert Answers: LC Troubleshooting and Best Practices

Expert Answers: LC Troubleshooting and Best Practices

Tap into expert LC guidance as Jim Grinias answers your questions on troubleshooting, method choices, and performance across workflows.

LC Troubleshooting and Best Practices

Secondary interactions in HPLC are unintended interactions between the sample and any component of the flow path. They can lead to poor peak shape, poor resolution, and low sample recovery – and make it hard to interpret your chromatogram! An ideal separation is based purely on the primary separation mechanism, whether that is hydrophobicity in reversed-phase or size in size-exclusion chromatography. In reality, secondary interactions can happen in any kind of separation. In this webinar, we will discuss how they happen and how we can mitigate them, with tools such as mobile phase modifiers or inert LC column technology.<blockquote>Presenter: Anne Blackwell, Ph.D. (Bio Columns Product Support Scientist, Agilent Technologies, Inc.)Anne’s work in analytical chemistry began at Saint Louis University where she received Bachelor Degree in both Chemistry and French. She then began her career in mass spectrometry at the University of Arizona where she received her Ph.D. under Dr. Vicki Wysocki, studying mass spectrometry and ion mobility as tools for structural determination of non-covalent protein assemblies. She joined Agilent in 2012, working as an application scientist on LC/QTOF and LC/IM/QTOF instrumentation specializing in metabolomics and lipidomics applications. In 2016 she moved into Agilent’s bio-columns group, providing customer support for biological chromatography applications.</blockquote>

Agilent Technologies: What’s Really Happening in Your Column: How Secondary Interactions Happen and What We Can Do about Them

What’s Really Happening in Your Column: How Secondary Interactions Happen and What We Can Do about Them

Learn how to identify and reduce secondary interactions in HPLC using mobile phase modifiers and inert column technology to improve peak shape and resolution.

What’s Really Happening in Your Column

The world of bioprocessing is evolving rapidly, demanding solutions that prioritize efficiency, flexibility, and innovation. In this webinar, discover how Shimadzu and Sepragen are reshaping the biopharmaceutical landscape with end-to-end solutions.Shimadzu's high-performance liquid chromatography (HPLC) for pharmaceutical research and quality control seamlessly complements Sepragen's Chromatography and Buffer Blending Systems, streamlining operations, boosting productivity, and reducing costs—helping biopharma manufacturers overcome today’s challenges and meet tomorrow’s needs.In this session, discover how Sepragen enables seamless scale-up across their downstream bioprocessing workflow, from their novel Superflo Radial Flow Column and QuantaSep Trusty Series bench-scale chromatography to the smart scale-up of automated chromatography. Learn how to optimize workflows, minimize footprints, ensure consistent product quality, and achieve effortless scalability— while maintaining full compliance throughout development and production.Be part of the conversation as we dive into real-world case studies and industry best practices, showcasing the transformative impact of these technologies. Register Now!<h4>Learning Objectives:</h4><ul><li>Explore how Quantasep empowers bioprocess engineers to address the challenges of modern biomanufacturing while achieving substantial cost savings.</li><li>Learn about cutting-edge bioprocessing solutions designed to optimize time efficiency and enable smarter upscaling.</li><li>Gain insights into maintaining compliance and product quality without compromise in your biomanufacturing processes.</li></ul><blockquote>Presenter: Liw Wan Tung (Manager (Biopharma/LC/Spectro) Shimadzu (Asia Pacific) Pte Ltd)Ms. Wan Tung is the Manager in charge of pharmaceutical market expansion and LC/Spectro products at Shimadzu Asia Pacific Pte Ltd. She specializes in providing technical consultation to researchers and laboratory professionals, helping them optimize methodologies to achieve accurate and reliable results. With over 15 years of experience in analytical science, Wan Tung is recognized as an expert in LC/SFC/MS technologies. Her expertise spans a broad spectrum of areas, including QAQC regulatory compliance, impurity analysis, drug discovery, and biopharmaceutical applications.Before joining Shimadzu, she began her career as a QA/QC chemist at a multinational pharmaceutical company. She holds a Bachelor's Honours degree in Chemistry from the University Tunku Abdul Rahman (UTAR).Presenter: Vinit Saxena (Founder &amp; CEO, Sepragen Corporation)Mr. Vinit Saxena is the founder and CEO of Sepragen Corporation, which specializes in innovative bio-processing equipment and consumables for producing and purifying biological drugs, nutritional ingredients, and vaccines. Sepragen's patented technologies are utilized globally, contributing to the production of over 40 FDA-approved drugs, including hundreds of millions of doses of two prominent COVID-19 vaccines. The company serves leading biopharma clients across four continents, including Abbvie, Amgen, Novartis, Sanofi, Takeda, Serum Institute and Biocon as well as major nutritional companies like Arla and Mead Johnson.With over 35 years of industry experience in marketing and manufacturing, Mr. Saxena holds degrees from IIT Kanpur, Syracuse University, and U.C. Berkeley. He has authored over 15 patents and numerous publications, and has been a prominent speaker at industry conferences. Additionally, he serves on the Scientific Advisory Board of The Keck Graduate Institute and has received several SBIR grants and the prestigious IR 100 award.</blockquote>

Shimadzu: Latest Biopharma Innovation: Autonomous, Scalable & Cost-Effective Solutions For Downstream Processing

Latest Biopharma Innovation: Autonomous, Scalable & Cost-Effective Solutions For Downstream Processing

Integrated bioprocessing with Shimadzu and Sepragen, covering scalable chromatography, workflow optimization, compliance, and real-world biopharma case studies.

Biopharma Scalable & Cost-Effective Solutions

Discover how to boost efficiency and simplify your LC-MS workflows with Evosep. Whether you’re just getting started or exploring new ways to streamline your proteomics setup, this session provides practical insights for users at any experience level. Learn how Evosep’s intuitive, automation-driven technology makes LC-MS–based proteomics accessible, reliable, and easy to integrate into your everyday lab routines.<blockquote>Speakers to be announced</blockquote>

Evosep Biosystems: Beginner’s guide to Evosep

Beginner’s guide to Evosep

Discover how to boost efficiency and simplify your LC-MS workflows with Evosep.

<header>AAV-based therapies continue to transform the gene therapy landscape—but as programs scale, challenges in purification and process optimization are becoming more complex.In this panel discussion, industry specialists will discuss the latest trends in AAV purification, including engineered capsid innovation, emerging strategies for efficient empty/full separation and polishing, and high-throughput analytical techniques to accelerate screening and development. Join us to gain practical insights and impactful strategies to help streamline AAV purification, enabling scalable, high-quality vector manufacturing.<h4>﻿Attend this webinar to:</h4><ul><li>Discover methods that could improve your AAV vector purification efficiency, purity, and yield</li><li>Learn from real-life insights shared and discussed by experienced AAV process and analytical development scientists</li><li>Anticipate upcoming challenges and potential solutions in your AAV purification and manufacturing process</li><li>Stay up-to-date on AAV-based therapeutics going into 2026</li></ul><div><div data-advanced-option="false" data-has-preview-image="false" data-video-id="" data-component-id="2110920040"><div style="max-width:1px;width:100%;" id="mediaContainerWrapper_2110920040"><div style="height:1px;width:1px;" data-external-url="" id="mediaContainer_2110920040" data-advanced-option="false" data-width="1" data-height="1" data-video-id="">&nbsp;</div></div></div></div></header><div><div><div data-advanced-option="false" data-has-preview-image="false" data-video-id="" data-component-id="2110920040"><blockquote><div>Presenter: Scott Jeffers, PhD (Chief Technical Officer at GenSight Biologics)</div><div>&nbsp;</div><div>Scott Jeffers, Ph.D., is Chief Technology Officer at GenSight Biologics, where he leads global CMC strategy for AAV-based gene therapy programs, including the commercial development of LUMEVOQ® for Leber Hereditary Optic Neuropathy. With over 20 years of experience in gene therapy and viral vector development, Dr. Jeffers has held senior roles at uniQure, Selecta Biosciences, and Brammer Bio, contributing to the launch of Hemgenix®, the first FDA-approved gene therapy for hemophilia B.</div>His scientific background includes doctoral research at Purdue University (three U.S. patents in viral vector design), discovery of the SARS-CoV receptor CD209L at the University of Colorado, and structural virology research at the Institut Pasteur, published in Science and PLOS Pathogens.Dr. Jeffers also founded CGT Resources Now LLC, advising biopharma companies on manufacturing strategy, comparability, and regulatory readiness. A frequent speaker at global bioprocessing conferences, he is recognized for advancing innovation and reliability in gene therapy manufacturing.Presenter:&nbsp;Andrew D. Tustian, PhD (Executive Director, Preclinical Manufacturing and Process Development at Regeneron Pharmaceuticals)Andrew leads the viral vector process development group at Regeneron Pharmaceuticals, focused on developing bioprocesses for Adeno-associated virus (AAV) based viral vectors to cure diseases such as Hemophilia B, congenital hearing loss, and Pompe disease. Andrew has worked at Regeneron since 2009. Prior to moving to gene therapy Andrew co-led the purification development group for protein therapeutics, working on Fc-fusion, monoclonal antibody, and bispecific processes. He has worked on the process development for multiple FDA-approved drugs including Dupixent®, Libtayo®, Inmazeb™, Kevzara®, Evkeeza™ and Praluent®, and helped develop the bispecific antibody platform at Regeneron. Andrew received his doctorate in biochemical engineering from University College London and his undergraduate masters in biochemistry from the University of Oxford.Presenter:&nbsp;Samarendra Mohanty, PhD (President and Chief Scientific Officer at Nanoscope Therapeutics)Dedicated to transforming scientific discoveries into life-changing therapies, Dr. Samarendra Mohanty co-founded Nanoscope Therapeutics and serves as president and chief scientific officer. With over 25 years of experience in biomedical sciences, Dr. Mohanty has co-founded several biotech and medical device companies focused on developing and commercializing innovative diagnostics and therapeutic technologies. His mission-driven work bridges cutting-edge science and patient impact, with a focus on neurological and ophthalmic disorders. He has held senior academic and research positions worldwide and is Principal Investigator for major NIH-funded projects. With over 200 international patents and publications in top journals like Nature, Dr. Mohanty remains a leader in biomedical innovation. He continues to innovate and serve on the Boards of many biotech companies.Presenter:&nbsp;Jayan Senaratne (Director, Downstream Process Development at MeiraGTx)Jayan Senaratne is Director of Downstream Process Development at MeiraGTx, where he leads the early and late-stage development of downstream and drug product processes for AAV gene therapies. With an EngD in Biochemical Engineering from University College London and a MEng in Chemical Engineering from the University of Nottingham, he brings deep technical expertise in the development of robust and efficient manufacturing processes for biopharmaceuticals. Before joining MeiraGTx in 2019, Jayan built his career in process development and optimisation at major biotech organisations, including the Centre for Process Innovation and MedImmune (AstraZeneca).Presenter:&nbsp;Pouria Motevalian (Process Development Director at Thermo Fisher Scientific)Pouria Motevalian is an accomplished leader in the biopharmaceutical industry with extensive experience in developing innovative biopharmaceutical products such as viral vectors, vaccines, plasmid DNA, and multispecific antibodies. He currently oversees a team of over 30 scientists, driving significant advancements in the development and commercialization of novel viral vectors. In his prior role at Pfizer, he contributed to the development and regulatory approval of key products like Prevnar 20 and the COVID-19 mRNA vaccine.</blockquote></div></div></div>

Thermo Fisher Scientific: Addressing AAV purification bottlenecks in 2026

Addressing AAV purification bottlenecks in 2026

Learn AAV purification strategies, from capsid design to empty/full separation and analytics, supporting scalable, high-quality gene therapy manufacturing.

Accurate mass fundamentals breaks down the barriers of complexity, demystifies misconceptions, and provides helpful tips on how to improve your data quality.Whether you already use accurate mass spectrometry and are looking to grow your knowledge or are currently working with nominal mass spectrometry and are thinking about making the switch, this webinar series will show you how accurate mass can simplify your workflow, clarify your data, and give you greater confidence in every result.Sign up today to discover the practical advantages of accurate mass spectrometry.<h4>Session 1: What it is and why it matters</h4><ul><li>Understand the theory behind mass accuracy and resolution</li><li>Learn what nominal mass can and cannot reveal</li><li>See real examples where mass accuracy changes data interpretation</li></ul>

SCIEX: Accurate mass fundamentals - What it is and why it matters

Accurate mass fundamentals - What it is and why it matters

Learn fundamentals of accurate mass spectrometry, understand mass accuracy, resolution, why accurate mass delivers clearer, reliable analytical results.

Pharma teams often face disconnected systems and slow decision-making. Paul Gillham will share how breaking down silos and creating a seamless data flow can transform operations. Discover how intuitive tools and smarter automation help teams work better together, speed up processes, and deliver consistent quality, without adding complexity.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://biopharmawebinars.com/webinars/developing-and-deploying-digital-ai-solutions-in-pharma-the-reality-vs-the-hype/#webinar_embed">Register for this webinar</a></blockquote><h4>What You’ll Learn</h4><ul><li>Easy ways to connect data and workflows for faster, smarter decisions.</li><li>How automation can boost efficiency while keeping people at the center.</li><li>Practical steps to build a future-ready, digital pharma environment.</li></ul><h4>Who Should Join</h4><ul><li>Pharma manufacturing and quality leaders looking for actionable solutions.</li><li>Digital transformation and automation champions.</li><li>Process engineers and Pharma 4.0 advocates.</li><li>Senior managers seeking strategies that deliver measurable results.</li></ul>Sponsored by SciY and followed by a live Q&amp;A session.<blockquote>Presenter: Paul Gillham (Innovations Director, SciY)Paul is the Director of Innovation at SciY where he leads a team of scientists, mathematicians and engineers. He has over twenty years of experience in the implementation of digitalization for life sciences, notably in the field of PAT (Process Analytical Technologies), with a focus on data management, analysis and communication. He read Physics at the University of Bath and has worked in different industries before joining Optimal, to work on the synTQ suite of software. In April 2022 Optimal was purchased and placed into the SciY group of companies, which has a broad portfolio of software applications within its data management platform. His main aim at SciY is to advance data use, analysis and to action control of our customer’s processes, based on understanding and good science.&nbsp;</blockquote>

SciY: From Silos to Synergy: How Smarter Data Flow Transforms Pharma

From Silos to Synergy: How Smarter Data Flow Transforms Pharma

Join webinar to learn how pharma teams can connect data, automate intelligently, accelerate decision-making. Practical Pharma 4.0 insights from SciY experts.

How Smarter Data Flow Transforms Pharma

Join us for this insightful session as we dive into the evolving landscape of metabolomics and lipidomics powered by high resolution mass spectrometry. Discover how cutting-edge advancements are transforming analytical workflows. Don’t miss this opportunity to gain practical knowledge that can elevate your research and accelerate meaningful scientific insights.<h4>Key highlights:</h4><ul><li>Sensitive detection</li><li>Structural elucidation</li><li>High-throughput quantitation</li></ul><blockquote>Speaker: Dr. Dipankar Malakar, Application Support Manager</blockquote>

SCIEX: Advancements for metabolomics and lipidomics workflows using high resolution mass spectrometry

Advancements for metabolomics and lipidomics workflows using high resolution mass spectrometry

Join us for this insightful session as we dive into the evolving landscape of metabolomics and lipidomics powered by high resolution mass spectrometry.

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