<p><strong>American postdoctoral researcher and ERA Postdoctoral Fellow William Morton came to CEITEC Masaryk University from the United Kingdom two years ago to join Robert Vácha’s research group. During his time in Brno, he focused on computational research of disordered proteins and experienced not only the Czech research environment, but also everyday life in the city. Now, as he prepares to return to the UK, he reflects on what CEITEC has given him, where he sees room for improvement, and why science depends not only on excellent results, but also on ordinary day-to-day conversations between researchers.&nbsp;</strong></p><h5><strong>When you were looking for your next research step, what made CEITEC stand out to you?</strong></h5><p>For me, the decision was primarily about people rather than place. I had already spent a month in Brno during my PhD, so I knew the environment and had a sense that I enjoyed both the institute and the city. But the key factor was the opportunity to work with <strong>Robert Vácha</strong>. I knew that his expertise aligned perfectly with where my research was heading, and I made a conscious decision not to apply elsewhere until I knew whether I could join his group. Once that opportunity came through, the decision was straightforward.</p><h5><strong>Before coming here, what were your expectations about Brno and CEITEC – and what turned out to be completely different, in a good way?</strong></h5><p>Honestly, I didn’t have strong expectations. I had seen Prague, but I knew Brno would be different, so I arrived with a fairly open mind. What surprised me most was how rich the city is despite its size. At first glance, Brno seems small, but it offers an incredible variety of communities, activities, and hidden places. Whatever your interests are, you can usually find people who share them and spaces that support them. That depth and accessibility of life here was something I didn’t expect at all.</p><h5><strong>How has your day-to-day life in Brno felt as an international researcher? If someone from abroad was hesitating about moving here, what would you tell them?</strong></h5><p>Day-to-day life can be quite intense, especially at the beginning. As an early-career researcher, you’re expected to quickly become independent, manage your own projects, build collaborations, and often mentor others at the same time. In my case, I arrived while still finishing my PhD, so I was navigating two major transitions at once, while also adapting to a new country.</p><p>That said, Brno is a place where this transition feels manageable. One of the reasons I’ve come to really enjoy living here is that despite its size, the city offers everything I need both professionally and personally. It’s easy to find communities, activities, and a good balance outside the lab.</p><p>If someone from abroad was hesitating about moving here, I’d say to expect a steep learning curve at first, but also a very rewarding experience once you settle in.</p><h5><strong>What has it been like working within your team here – how would you describe the collaboration and overall atmosphere?</strong></h5><p>Within Robert Vácha’s group, one of the biggest strengths is the breadth of expertise. There’s a really wide range of computational techniques in the group, and people are generally very willing to help each other and share knowledge. That creates an environment where you can learn a lot very quickly.</p><p>One thing I realised during my time here is how important it is to create spaces where researchers – not only group leaders, but also early-career people working directly on experiments and data – can openly discuss and challenge each other’s ideas. Those conversations are incredibly valuable. They help people improve their research, prepare for criticism, and feel more connected to the wider scientific community around them. And I don’t think this necessarily starts with big institutional goals or public-facing topics.</p><p>It mostly requires cooperation, communication, and leadership that creates opportunities for those interactions to happen regularly.</p><h5><strong>I heard you were receiving rather unique ‘packages’ from your neighbours – can you tell me a bit about that experience and what it says about life here?</strong></h5><p>Yes – this was one of my favourite parts of living here. I would occasionally get a knock on my window, and there would be jars of tomatoes, homemade syrup, pastries, or herbs from my neighbour.</p><p>More broadly, I found that people in the Czech Republic are incredibly kind, even without a shared language. I had many experiences – whether with neighbours or complete strangers – where people were welcoming and generous without expecting anything in return. That says a lot about the culture here.</p><h5><strong>Finding housing is often one of the first challenges when moving abroad – how was your experience in Brno?</strong></h5><p>I was fortunate to receive help with housing from CEITEC Welcome Officer, so my personal experience was relatively smooth. In fact, the apartment I originally stayed in is now being used by another postdoc arriving to Robert Vácha’s group, which says a lot about how important that kind of support network can be.</p><p>At the same time, I know from others that finding housing in Brno can be quite difficult, especially for non-EU researchers who need formal rental contracts for visa and administrative purposes. There are many informal rental options available, but they don’t always meet the official requirements, which makes the whole process more stressful and complicated.</p><p>I think departments like the CEITEC Welcome Office do a huge amount to help international researchers navigate this, but it also shows how important it is to make the system itself more accessible for highly qualified people coming from abroad.</p><h5><strong>Did you find it difficult to settle in without speaking Czech, or were you able to navigate most situations comfortably in English?</strong></h5><p>In general, it’s very manageable to live in Brno using English. You might encounter occasional misunderstandings, but if you approach situations with patience and flexibility, it works well.</p><p>I found that most interactions were positive, and people were generally willing to help – even when communication wasn’t perfect.</p><h5><strong>You’re now moving on to your next position – what are you taking with you from Brno into this next chapter?</strong></h5><p>I’m taking both professional experience and a broader perspective on research environments. The experience of working independently, managing multiple responsibilities, and navigating an international setting will be valuable wherever I go next.</p><h5><strong>If a young researcher was considering CEITEC, what would be your honest pitch to them?</strong></h5><p>My honest advice would be to focus on finding the right research group – and by that, I mean the whole environment around you. Not only the supervisor, but also the PhD students, technicians, and postdocs you’ll work with every day. Those relationships shape your experience much more than people sometimes expect.</p><p>I think if you find the right fit, you can absolutely succeed at CEITEC, have a really wonderful experience, and take advantage of everything the institute offers.</p><p>I would also encourage people to take the interview process seriously – not just as candidates trying to get a position, but also as an opportunity to evaluate whether the group feels right for them. If that fit is there, CEITEC offers strong support and a great environment to grow.</p>

William S. Morton: The Best Part of Living in Brno? Probably the Tomatoes Through the Window 

<p><strong>If you’re considering a nitrogen generator for your lab, one of the first questions that often comes up is: “What kind of maintenance am I signing up for?”</strong></p><p>It’s a fair concern. Many labs are used to equipment that requires frequent service, costly parts, or ongoing vendor support. The good news is that the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1902"><strong> NITRO-GEN nitrogen generator</strong></a> was specifically designed to keep <strong>maintenance simple, infrequent, and affordable</strong>.</p><p>In fact, compared to many laboratory nitrogen generators, it offers one of the<strong> lowest overall maintenance burdens</strong>, making it an easy transition for labs looking to move away from gas cylinders without adding complexity.</p><h3><strong>Simple Maintenance, Minimal Time Commitment</strong></h3><ul><li>For most labs, maintaining the NITRO-GEN comes down to one straightforward task: <strong>periodic filter replacement</strong>.</li></ul><p>The system uses a<strong> two-stage filtration setup</strong>. The coarse filter, which serves as the first stage, captures larger particulates from your compressed air supply and protects the rest of the system. This element should be replaced about <strong>once per year</strong>. Downstream, the fine filter removes smaller contaminants and typically lasts much longer, generally <strong>2–5 years</strong>, depending on usage and air quality.</p><p>Replacing the coarse filter annually is key. Not only does it maintain consistent nitrogen output, but it also helps extend the life of the fine filter, keeping overall maintenance both minimal and cost-effective.</p><p>Actual filter lifespan will vary depending on how heavily the generator is used and how clean your incoming compressed air is. However, most labs find that these intervals are more than sufficient to keep the system running smoothly. And if you ever need to access the filters, it’s quick and easy—just unscrew the rear plate of the generator to reach the filter elements. <strong>No specialized tools or service calls required</strong>.</p><h3><strong>Low Maintenance Costs Without the Surprises</strong></h3><p>Beyond the low frequency of maintenance, the cost of keeping a NITRO-GEN running is also very low.</p><p>With the coarse filter replaced annually and the fine filter only occasionally,<strong> typical annual maintenance costs are under $100</strong>. That’s a fraction of what many labs expect when evaluating nitrogen generation systems, and significantly lower than the ongoing costs associated with gas cylinders or more complex generators.</p><p>There are also clear indicators when maintenance is needed. If you notice a drop in nitrogen flow or that the drain port is no longer draining properly, it’s likely time to replace one or both filter elements. These simple visual and performance cues make it easy to stay ahead of maintenance without overthinking it.</p><h3><strong>How NITRO-GEN Compares to Other Nitrogen Generators</strong></h3><p>Not all nitrogen generators are created equal—especially when it comes to maintenance.</p><p>Many laboratory nitrogen generators rely on more complex technologies such as pressure swing adsorption (PSA) or multi-stage membrane systems. While these approaches are effective at producing nitrogen, they often come with additional maintenance considerations that aren’t always obvious upfront.</p><ul><li><strong>PSA systems</strong>, for example, use adsorption columns filled with specialized materials along with valves that continuously switch during operation. Over time, these components can require servicing or replacement, and the systems are highly sensitive to moisture and oil in the compressed air supply, making air preparation and filtration even more critical.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/how-do-membrane-nitrogen-generators-work"><strong>Membrane-based generators </strong></a>are somewhat simpler, but still depend heavily on consistent air quality and multiple pre-filtration stages. If those upstream systems aren’t maintained properly, performance can decline and internal components may need replacement.</li></ul><p>Across both types of systems, maintenance often extends beyond the generator itself. Supporting equipment like air compressors, dryers, and filtration systems typically require their own upkeep, adding to the total cost and complexity of ownership.</p><p><strong>The NITRO-GEN was designed with a different philosophy:</strong> keep nitrogen generation simple and accessible for the lab environment. Instead of relying on complex internal systems, it focuses on a streamlined design where routine upkeep is limited to quick, infrequent filter changes. There are no adsorption beds to replace, no complex valve systems to service, and no specialized maintenance procedures required.</p><h3><strong>A Simpler Alternative to Gas Cylinders</strong></h3><p>For labs currently relying on nitrogen cylinders, <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/how-much-could-i-save-switching-to-a-nitrogen-generator"><strong>switching to a generator</strong></a> can feel like trading one set of responsibilities for another. But with the NITRO-GEN, that tradeoff is minimal.</p><p>Instead of managing tank deliveries, monitoring supply levels, and handling cylinder changes, you get a reliable, on-demand nitrogen source with only occasional, low-cost maintenance. The result is a more streamlined workflow, fewer interruptions, and greater long-term cost control.</p><h3><strong>The Bottom Line</strong></h3><p>If maintenance concerns have been holding you back from switching to a nitrogen generator, the NITRO-GEN was built to eliminate that barrier.</p><p>With one quick annual filter replacement, infrequent long-term maintenance, and annual costs under $100, it’s one of the simplest and most cost-effective nitrogen generation solutions available for laboratory use.</p>

How Much Maintenance Does the NITRO-GEN generator really require?

<p><strong>🎤Amanda Guiraldelli Mahr&nbsp;</strong></p><p><strong>Amanda shares insights from her work across small molecules, peptides, proteins, and biopharmaceuticals, including her experience with LC-MS, metabolomics, impurity analysis, and oligonucleotide therapeutics.</strong></p><h4><strong>What You’ll Learn in This Episode:</strong></h4><ul><li>What AQbD actually means (in simple terms)</li><li>How DOE differs from traditional one-factor-at-a-time experiments</li><li>Why modern labs are shifting toward predictive, data-driven method development</li><li>The biggest benefits of DOE: more insight with fewer experiments*</li><li>Real-world example: optimizing oligonucleotide impurity separations using AQbD&nbsp;</li><li>Key LC parameters that impact performance (pH, gradient, column, temperature)</li><li>Challenges of working with large molecules vs. small molecules</li><li>Tools and software for DOE and chromatographic modeling</li><li>How AI and modeling are shaping the future of chromatography</li></ul><blockquote><p><strong>Key Takeaway: </strong>“Shift your mindset from testing to understanding.”</p></blockquote><h4><strong>Who This Episode Is For:</strong></h4><ul><li>Undergraduate and graduate chemistry students</li><li>Analytical scientists and method developers</li><li>Pharma &amp; biopharma professionals</li><li>Anyone working with LC, LC-MS, or chromatography method development</li></ul><h3><strong>Video Transcription</strong></h3><p>In this episode of <i>Concentrating on Chromatography</i>, David interviews <strong>Amanda Guiraldelli Mahr</strong>, an analytical chemist whose career has spanned pharmaceuticals, metabolomics, regulatory science, mass spectrometry, and biopharmaceutical analysis. Amanda discusses her journey from pharmacy and biochemistry studies to working with chromatography and mass spectrometry in academic, regulatory, and industrial environments.</p><p>The conversation focuses on the growing role of <strong>Analytical Quality by Design (AQbD)</strong>, <strong>Design of Experiments (DOE)</strong>, predictive modeling, and the future integration of artificial intelligence into chromatographic method development. Amanda also shares practical insights into method development for small molecules, peptides, proteins, oligonucleotides, and emerging therapeutics.</p><h4><strong>Early Career and Introduction to Chromatography</strong></h4><p>Amanda's interest in analytical chemistry began during her studies in pharmacy and biochemistry. An internship focused on chromatography for botanical medicine analysis introduced her to separation science and inspired her to pursue a PhD in analytical chemistry.</p><p>During her doctoral research, she worked extensively with <strong>LC-MS-based metabolomics</strong>, which led her to explore experimental design approaches and method development strategies. Her passion for chromatography and mass spectrometry deepened further while working at the United States Pharmacopeia (USP), where she routinely applied chromatographic and mass spectrometric techniques to characterize:</p><ul><li>Small-molecule drug substances</li><li>Pharmaceutical ingredients</li><li>Dietary supplements</li><li>Botanical products</li><li>Peptides</li><li>Proteins</li></ul><p>Her work increasingly involved understanding chromatographic separation mechanisms through experimental design and modeling approaches.</p><h4><strong>Expanding into Proteomics, Nitrosamines, and Biopharmaceuticals</strong></h4><p>Amanda's scientific curiosity led her beyond traditional small-molecule analysis.</p><p>Throughout her career she gained experience in:</p><ul><li>High-resolution mass spectrometry</li><li>Structural elucidation</li><li>Proteomics</li><li>Bioanalysis</li><li>Trace impurity analysis</li><li>Nitrosamine monitoring</li><li>Oligonucleotide therapeutics</li><li>Large proteins and peptides</li></ul><p>She worked with advanced fragmentation techniques including:</p><ul><li>CID (Collision-Induced Dissociation)</li><li>HCD (Higher-Energy Collisional Dissociation)</li><li>UV Photodissociation</li></ul><p>Later, she became involved in developing highly sensitive LC-MS and GC-MS methods for monitoring <strong>nitrosamines</strong>, a major regulatory concern due to their genotoxic potential. She also trained regulatory agencies in mass spectrometry applications and method validation before moving into biopharmaceutical research and contract research activities focused on proteins, peptides, oligonucleotides, and RNA therapeutics.</p><h4><strong>What Does a Chromatographer Do Every Day?</strong></h4><p>When asked to summarize the day-to-day work of a chromatographer, Amanda highlighted three core activities:</p><ul><li>Designing experiments</li><li>Troubleshooting analytical methods</li><li>Interpreting unexpected results</li></ul><p>She emphasized that a large portion of her work involves data analysis and learning from experimental outcomes in order to optimize methods and better understand chromatographic behavior.</p><h4><strong>Understanding Analytical Quality by Design (AQbD)</strong></h4><p>One of the central topics of the discussion was <strong>Analytical Quality by Design (AQbD)</strong>.</p><p>Amanda describes AQbD as a systematic approach for understanding how analytical methods perform and identifying factors that influence method variability.</p><p>Key objectives of AQbD include:</p><ul><li>Identifying critical method parameters</li><li>Understanding sources of variability</li><li>Building robustness into methods from the beginning</li><li>Improving method reliability throughout its lifecycle</li></ul><p>Rather than correcting problems after implementation, AQbD focuses on proactively designing analytical procedures that consistently deliver high-quality results.</p><p>Amanda noted that AQbD principles are strongly linked to pharmaceutical quality frameworks established through ICH guidelines such as:</p><ul><li>ICH Q8 (Pharmaceutical Development)</li><li>ICH Q14 (Analytical Procedure Development)</li></ul><p>These guidelines have significantly influenced modern pharmaceutical method development practices.</p><h4><strong>AQbD vs. Traditional Trial-and-Error Development</strong></h4><p>Amanda contrasted AQbD with the traditional <strong>one-factor-at-a-time (OFAT)</strong> approach.</p><h5><strong>Traditional Approach</strong></h5><ul><li>Changes one parameter at a time</li><li>Provides limited understanding of interactions</li><li>Often misses important relationships between variables</li></ul><h5><strong>AQbD Approach</strong></h5><ul><li>Uses multivariate experimental designs</li><li>Studies multiple variables simultaneously</li><li>Reveals interactions between parameters</li><li>Enables predictive modeling</li><li>Defines robust operating ranges</li></ul><p>According to Amanda, AQbD provides significantly more knowledge about method behavior while reducing unnecessary experimentation.</p><h4><strong>Design of Experiments (DOE): A Core AQbD Tool</strong></h4><p>Amanda clarified an important misconception:</p><p><strong>DOE is not the same as AQbD.</strong></p><p>Instead:</p><ul><li>AQbD is a broader philosophy and lifecycle approach.</li><li>DOE is one of the most powerful tools used within AQbD.</li></ul><p>DOE helps scientists:</p><ul><li>Identify critical factors</li><li>Evaluate interactions</li><li>Map regions of good and poor performance</li><li>Support risk assessment</li><li>Build predictive models</li></ul><p>However, AQbD also includes risk management, control strategies, and lifecycle monitoring beyond experimental design itself.</p><h4><strong>Practical Advice for Scientists New to DOE</strong></h4><p>For scientists interested in implementing DOE, Amanda recommends starting with simple screening studies.</p><p>Examples include evaluating:</p><ul><li>LC column chemistry</li><li>Mobile-phase pH</li><li>Organic modifiers</li><li>Temperature</li></ul><p>Important responses to monitor may include:</p><ul><li>Resolution</li><li>Peak tailing</li><li>Selectivity</li><li>Efficiency</li></ul><p>She encourages scientists to focus not only on collecting data but on understanding the patterns revealed by experimental results. As experience grows, more advanced optimization studies and predictive models can be developed.</p><h4><strong>Which LC Parameters Matter Most?</strong></h4><p>Amanda emphasized that critical parameters depend on:</p><ul><li>Sample type</li><li>Separation mode</li><li>Intended method purpose</li><li>Development stage</li></ul><p>For reversed-phase LC, common screening parameters include:</p><ul><li>Column chemistry</li><li>Mobile-phase pH</li><li>Organic solvent composition</li><li>Temperature</li></ul><p>For ion-pair chromatography, additional factors such as:</p><ul><li>Ion-pairing reagent type</li><li>Ion-pairing reagent concentration</li></ul><p>can strongly influence selectivity and retention behavior.</p><p>She stresses that proper risk assessment should always guide parameter selection.</p><h4><strong>Case Study: Oligonucleotide Impurity Profiling</strong></h4><p>Amanda shared an example involving impurity analysis of <strong>oligonucleotide therapeutics</strong>.</p><p>One major challenge is that impurities are often structurally very similar to the active pharmaceutical ingredient (API), making chromatographic separation difficult.</p><p>Using AQbD and DOE, her team systematically evaluated:</p><ul><li>LC column chemistries</li><li>Ion-pairing reagents</li><li>Temperature</li><li>Gradient conditions</li></ul><p>A desirability-function approach allowed simultaneous optimization of multiple critical resolutions rather than focusing on a single impurity pair.</p><p>This strategy enabled the development of an LC-UV method capable of separating impurities that might otherwise require LC-MS for quantification.</p><h4><strong>The Challenges of Large Molecules</strong></h4><p>Amanda identifies large biomolecules as some of the most challenging analytes in chromatography.</p><p>Compared with small molecules, proteins and oligonucleotides exhibit:</p><ul><li>Slower mass transfer kinetics</li><li>Peak broadening</li><li>Reduced sensitivity</li><li>Greater sensitivity to system-related factors</li></ul><p>Even seemingly minor differences can affect performance, including:</p><ul><li>Dwell volume</li><li>Tubing dimensions</li><li>System dispersion</li></ul><p>She also highlighted non-specific adsorption as a major challenge.</p><p>Examples include:</p><h5><strong>Oligonucleotides</strong></h5><p>Negatively charged phosphate backbones can interact strongly with metal surfaces, reducing recovery and reproducibility.</p><h5><strong>Peptides</strong></h5><p>Basic amino acid residues may interact with residual silanol groups on silica-based stationary phases, affecting chromatographic performance.</p><p>These challenges have driven innovation in:</p><ul><li>Low-adsorption LC systems</li><li>Surface-modified columns</li><li>Hybrid particle technologies</li><li>Improved consumables and hardware design</li></ul><h4><strong>Software and Modeling Tools</strong></h4><p>Amanda discussed several software platforms commonly used for DOE and method optimization:</p><h5><strong>DOE Software</strong></h5><ul><li>Design-Expert</li><li>JMP</li><li>Minitab</li><li>MODDE</li></ul><h5><strong>Chromatographic Modeling Software</strong></h5><ul><li>DryLab</li><li>ACD/AutoChrom</li><li>ChromSword</li></ul><p>She also noted that modern software increasingly integrates directly with chromatographic data systems, enabling automated experiment execution and data analysis.</p><h4><strong>Essential Skills Beyond Instrument Operation</strong></h4><p>According to Amanda, successful chromatographers need more than technical instrument expertise.</p><p>Key skills include:</p><ul><li>Chromatographic fundamentals</li><li>Statistics</li><li>Chemometrics</li><li>Data interpretation</li><li>Predictive modeling</li><li>Risk assessment</li></ul><p>She emphasized that statistical literacy is particularly important for implementing AQbD principles and validating predictive models.</p><h4><strong>The Future of Chromatography</strong></h4><p>Looking ahead, Amanda is particularly excited about the integration of:</p><ul><li>Artificial intelligence</li><li>Predictive modeling</li><li>Knowledge-driven method development</li></ul><p>She believes these technologies will help scientists better understand separation mechanisms and accelerate development of methods for increasingly complex therapeutics.</p><p>Ultimately, she expects analytical science to play an even more central role in pharmaceutical development, manufacturing, and quality assurance.</p><h4><strong>Conclusion</strong></h4><p>Amanda's career illustrates how chromatography has evolved from traditional small-molecule analysis into a highly interdisciplinary field spanning proteomics, biopharmaceuticals, oligonucleotide therapeutics, and advanced regulatory science. Her experience demonstrates the value of combining chromatographic expertise with AQbD principles, DOE, risk assessment, and predictive modeling.</p><p>As analytical challenges continue to grow in complexity, approaches that emphasize understanding, prediction, and quality by design are becoming increasingly important. The future of chromatography is likely to be shaped by data-driven decision making, advanced modeling, and artificial intelligence—tools that will enable scientists to develop more robust, efficient, and reliable analytical methods.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

AQbD Explained: How Design of Experiments is Transforming Chromatography

Article

Product

Video

Scientists

Interview

Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

CEITEC

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Tosoh Bioscience

Agilent Technologies

Phenomenex

Thermo Fisher Scientific

SelectScience

Shimadzu Corporation

Waters Corporation

<p><strong>In the race to launch GLP‑1 generics and novel agonists, complex impurity analyses directly impact your speed to market. Conventional columns often obscure peaks of critical impurities, leading to costly rework and increased regulatory scrutiny.</strong></p><p>Join us and learn about new analytical tools designed to streamline your process and deliver reproducible, high-resolution separations of GLP‑1 receptor agonists (GLP-1 RAs) and insulin-related impurities without relying on suppressive mobile‑phase additives.</p><p>Learn how improved resolution can accelerate method development, increase confidence in your results, and support faster time to market for your GLP-1 RA and insulin products.</p><h4><strong>In this webinar you will learn:</strong></h4><ul><li>How to gain greater resolution for your GLP-1 RA and insulin impurity analysis separations</li><li>How to achieve up to 3x faster separations</li><li>How to identify subtle impurities, such as single amino-acid substitutions and deamidation, beyond the detection limits of conventional reversed-phase technologies</li></ul><h4><strong>Who should attend</strong></h4><p><strong>This webinar is designed for you if you work in:</strong></p><ul><li>Analytical development</li><li>Process development</li><li>R&amp;D</li><li>CXOs dealing with GLP-1 RAs or insulin</li></ul><blockquote><p><strong>Presenter: </strong>Michael Zagieboylo (Principal Product Manager, Waters Corporation)</p><p>Michael Zagieboylo is a Principal Product Manager at Waters Corporation whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of biological molecules, focusing on peptide and protein separations.</p></blockquote><blockquote><p><strong>Presenter: </strong>Steve Shiner (Senior Manager, R&amp;D for Biopharma Solutions, Waters Corporation)</p></blockquote>

Clear the Noise: Improving Resolution of GLP-1 Receptor Agonist and Insulin Impurities

Resolve GLP-1 receptor agonist and insulin impurities faster. Discover analytical tools for high-resolution separation of deamidation and amino-acid changes.

Improving Resolution of GLP-1 and Insulin Impurities

<p><strong>Advancing protein and viral vector characterization requires detection strategies that deliver higher sensitivity without adding complexity to established workflows. Traditional UV detection can introduce challenges such as labelling steps, baseline drift, and lower signal‑to‑noise ratios, making it harder to resolve critical quality attributes in capillary electrophoresis–sodium dodecyl sulfate (CE‑SDS) as workflows scale and regulatory expectations increase.</strong></p><p>In this SelectScience® TechTalk, Staff Application Scientist, Peter Holper, with 15+ years of biopharma industry experience, will demonstrate how native fluorescence detection (NFD) on the BioPhase 8800 system is raising the bar for CE-SDS analysis. By harnessing the natural fluorescence of aromatic amino acids, NFD eliminates protein labeling while delivering improved signal-to-noise ratios and cleaner baselines.</p><p>You will also discover how 8‑capillary parallel processing accelerates throughput across workflows such as CE‑SDS purity analysis and AAV capsid protein characterization, supported by real data showing accuracy and repeatability across detection modes. With streamlined sample preparation and stable baselines, your lab can get to answers faster and with greater confidence.</p><h5><strong>Certificate of attendance</strong></h5><p>If you attend the live TechTalk, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.</p><p>If you view the on-demand TechTalk, you can request a certificate of attendance by emailing editor@selectscience.net.</p><h5><strong>TechTalk details</strong></h5><ul><li><strong>Cost: Free to attend</strong></li><li><strong>Location: Online</strong></li><li><strong>Duration: 20 minutes</strong></li></ul><p><i>Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.</i></p><h5><strong>This event is perfect for:</strong></h5><ul><li>Analytical development scientists</li><li>Process development scientists</li><li>CMC Directors</li></ul><blockquote><p><strong>Speaker:</strong> Peter Holper (Staff Application Scientist, SCIEX)</p><p>Peter Holper earned his degree in Biochemistry and Cell Biology from the University of California, San Diego. He has over 15 years of experience in biopharma, including as an analytical chemist at Eli Lilly and Company where he was responsible for developing the analytical control strategy for bioproducts. Holper currently works at SCIEX where he is responsible for developing and optimizing CE applications and providing customer demo sample analysis support.</p></blockquote>

SelectScience: Native fluorescence detection for high-throughput CE-SDS and AAV characterization in drug development SCIEX

Native fluorescence detection for high-throughput CE-SDS and AAV characterization in drug development

Discover native fluorescence detection (NFD) on the BioPhase 8800 system for label-free, high-throughput CE-SDS purity analysis and AAV characterization

Native Fluorescence for CE-SDS and AAV Characterization

<p><strong>On June 23, Abbie Breton, Applications Development Manager, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts. Yuliya McAnany, Applications Scientist – Process Resins at Tosoh Bioscience, will showcase a suite of high-performance tools for reliable separation and analysis of AAV particles. Yuliya will start by discussing Tosoh’s novel full capsid enrichment method for TOYOPEARL GigaCap Q-650S resin combined with choline chloride, achieving 90% purity and 75% recovery. The conversation will include a look into Tosoh’s analytical toolkit for increased reliability, and improved sensitivity for critical quality monitoring.</strong></p><p>Participants will learn how to simplify the development of a high-performing enrichment step and will learn how Tosoh’s TSKgel Q-STAT, TSKgel GMPWxl, and LenS3 MALS detector can be used to advance quality monitoring of AAV products.</p><p>After the webinar, ask your questions during the live Q&amp;A session.&nbsp;</p><blockquote><p><strong>Presenter:</strong> Abbie Breton (Applications Development Manager, Tosoh Bioscience)</p></blockquote><blockquote><p><strong>Presenter:</strong> Yuliya McAnany (Applications Scientist – Process Resins, Tosoh Bioscience)</p></blockquote>

Tosoh Bioscience: Tosoh Tuesday - Exploring the AAV Toolkit

Tosoh Tuesday - Exploring the AAV Toolkit

Advance your AAV quality monitoring. Explore Tosoh's full capsid enrichment methods using TOYOPEARL resins and precise TSKgel SEC analysis with MALS detection

Exploring the AAV Toolkit: Tosoh Chromatography Webinar

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>GLP-1 receptor agonists are among the most impactful peptide therapeutics, but their structural complexity presents significant analytical challenges. From sequence modifications to lipidation and manufacturing variability, achieving confident characterization requires advanced approaches.</strong></p><p>Join us to learn how a streamlined LC-MS workflow can simplify GLP-1 analysis. This session will highlight integrated strategies combining UHPLC separation, high-resolution mass spectrometry, and automated data analysis to support robust characterization and impurity monitoring of peptides such as semaglutide and tirzepatide.</p><h5><strong>What You Will Learn</strong></h5><ul><li>How to optimize LC-MS workflows for comprehensive GLP-1 peptide characterization</li><li>Practical strategies for improving separation of closely related peptide species</li><li>Approaches to enhance sequence coverage using advanced fragmentation techniques</li><li>Methods for confident impurity profiling and structural heterogeneity assessment</li></ul><p>Secure your spot today and gain practical insights to simplify complex GLP-1 analysis.</p><blockquote><p><strong>Presenter: </strong>Dr. Sara Carillo (Bioanalytical Research Lead, NIBRT)</p><p>Dr. Carillo specializes in structural characterization of complex biomolecules using mass spectrometry and NMR. At NIBRT, she develops advanced analytical strategies to better understand biomanufacturing processes and the structural complexity of biopharmaceuticals.</p></blockquote><blockquote><p><strong>Presenter: </strong>Dr. Kai Sheffler (Senior Manager, Product Manager for BioPharma Workflows, Thermo Fisher Scientific)</p><p>Dr. Scheffler is a biochemist specializing in LC-MS technologies for biopharmaceutical analysis. His expertise includes peptide mapping, intact mass analysis, and impurity profiling using HRAM MS. He focuses on developing LC-MS solutions for comprehensive characterization and quality assessment of biopharmaceuticals.</p></blockquote><blockquote><p><strong>Moderator:</strong> Dr. Kelly Broster (Sr Manager, Market Development &amp;&nbsp;Collaborations, Pharma Biopharma, Thermo Fisher Scientific)</p></blockquote></div></div></div>

Thermo Fisher Scientific: Simplifying Complexity in GLP-1 Receptor Agonist Analysis: From Structural Characterization to Impurity Monitoring

Simplifying Complexity in GLP-1 Receptor Agonist Analysis: From Structural Characterization to Impurity Monitoring

Optimize LC-MS workflows for GLP-1 analysis. Master impurity profiling, peptide separation, and structural characterization of semaglutide and tirzepatide

Simplifying GLP-1 Agonist Analysis & Impurity MS

<h4><strong>One webinar, two viewing options</strong></h4><p><strong>We know you're busy, select a viewing session that works best for you.</strong></p><h5><br><strong>Viewing Options</strong></h5><p><strong>Session 1</strong></p><ul><li>Tuesday, June 23, 2026</li><li>Los Angeles: 10:00 AM PT | New York: 1:00 PM EST</li><li>Mexico City: 12:00 PM CDT</li></ul><p><strong>Session 2</strong></p><ul><li>Wednesday, June 24, 2026</li><li>London: 9:00 AM BST | Berlin: 10:00 AM CEST&nbsp;</li><li>Mumbai: 1:30 PM IST</li></ul><p>Supported Liquid Extraction (SLE) for the cleanup of biological matrices has several advantages over Solid Phase Extraction (SPE). SLE requires fewer steps and less method development compared to SPE. For most samples that can be processed by liquid-liquid extraction, SLE can usually be used to provide cleaner and faster solvent partitioning. Historically, to get the cleanest SLE extracts, halogenated solvents like dichloromethane were used for elution of target analytes from the sorbent. A supported liquid extraction (SLE) approach that produced clean extracts with high recoveries and low matrix interferences using ethyl acetate, a more sustainable organic solvent, will be presented. Results for steroids, methylmalonic acid (MMA), and underivatized estrogens in human serum, in addition to optimized LC-MS/MS conditions and LC column considerations, are also discussed.</p><h4><strong>Key Learning Points</strong></h4><ul><li>Discover how SLE provides clean extracts for LC-MS/MS quantitation at low concentrations.</li><li>Describe conditions for the extraction and analysis of MMA from human serum.</li><li>Define method parameters for low-level quantitation of estrogens without derivatization.</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>All clinical, CROs, and forensic/toxicology analysts using LC/MS-MS.</li></ul><blockquote><p><strong>Presenter: </strong>Stephanie J Marin (Sr. Global Market Development Manager - Applied Markets, Phenomenex)</p><p>Stephanie J. Marin is the Clinical and Forensics Global Market Development Manager at Phenomenex. She received her Ph.D. in chemistry from Arizona State University and has expertise in sample preparation, liquid chromatography, and mass spectrometry. She has over 10 years of experience developing and validating clinical methods from her tenure at the ARUP Institute for Clinical and Experimental Pathology. Dr Marin has worked in marketing, product development, and applications development, in addition to customer-facing roles in customer and technical support, service, and customer education and training. She has also held positions at Hamilton Company, Selerity Technologies, and Biotage, and in analytical services for specialty chemicals and polymers at Rohm and Haas (now Dow). She was also a supervisor at an EPA-certified laboratory. She is the author of over 30 peer-reviewed publications, book chapters, and over 100 abstracts presented at national and international meetings.&nbsp;</p></blockquote>

Phenomenex: Sustainable Strategies for High Sensitivity Analysis for Clinical Research LC-MS/MS Methods 

Sustainable Strategies for High Sensitivity Analysis for Clinical Research LC-MS/MS Methods

Advance clinical LC-MS/MS analysis. Discover sustainable Supported Liquid Extraction (SLE) methods for steroids, MMA, and underivatized estrogens in serum

Sustainable SLE and LC-MS/MS for Clinical Analysis

<p><strong>Advances in metabolomics and lipidomics are generating unprecedented volumes of complex data, but confident identification and biological interpretation of small molecules remain significant challenges. Limitations in annotation, data dimensionality, and reproducibility can slow discovery and reduce confidence in downstream decision‑making across clinical, translational, and applied research.</strong></p><p>In this live broadcast event, leading experts explore how next‑generation mass spectrometry technologies, ion mobility, isotope‑based filtering, and computational tools are helping researchers move from complex datasets to confident insight. Through two short, focused scientific talks, speakers will share real‑world examples of how multi‑dimensional data and advanced workflows are being used to improve metabolite and lipid annotation, accelerate small‑molecule discovery, and enable more robust and reproducible analyses.</p><p>Broadcast as a single, high‑impact session and presented in partnership with Bruker, this webinar provides practical and conceptual guidance for scientists looking to push the boundaries of metabolomics and lipidomics research using modern analytical platforms such as timsMetabo™.</p><h5><strong>This broadcast is designed for:</strong></h5><ul><li>Scientists using mass spectrometry in metabolomics, lipidomics, and small‑molecule analysis</li><li>Researchers in clinical, translational, biopharmaceutical, and toxicology settings</li><li>Teams working in biomarker discovery, exposomics, and pathway analysis</li><li>Lab leaders evaluating next‑generation MS and ion mobility platforms</li></ul><blockquote><p><strong>Speaker: </strong>Dr. Michael Witting (Deputy Head Metabolomics and Proteomics Core &amp; Executive Manager Metabolomics, Helmholtz Munich)</p><p><strong>Speaker:</strong> Prof. Pieter Dorrestein (Skaggs School of Pharmacy and Pharmaceutical Sciences, Departments of Pharmacology and Pediatrics)</p><p><strong>Speaker: </strong>Pelle Simpson (Principal Scientist, Enveda)</p><p><strong>Moderator</strong>: Cameron Smith-Craig (Pharma and Applied Sciences Editor, SelectScience)</p></blockquote>

Bruker: Next‑generation metabolomics: From annotation to discovery at scale

Next‑generation metabolomics: From annotation to discovery at scale

Advance small-molecule discovery with Bruker's next-gen metabolomics. Learn how ion mobility and timsMetabo™ improve metabolite and lipid annotation

Next-Generation Metabolomics and Lipidomics Discovery

<h3><strong>Stop Guessing, Start Separating - A Smarter Path to Robust LC Methods</strong></h3><h4><strong>Overview</strong></h4><p>Developing robust LC methods doesn’t need to rely on trial and error. In this webinar, we introduce a systematic, tiered approach to chromatographic method development - from verifying system health and understanding instrument dispersion to screening multiple column chemistries and optimizing for selectivity and robustness.</p><p>Through a real-world case study, we demonstrate how to separate a complex steroid mixture using six different column chemistries, including our new ACQUITY™ Biphenyl RP column, which delivers notable selectivity advantages for this challenging application.</p><p>Join us to see how a structured screening strategy can accelerate method development and help you make confident, data-driven decisions for your next chromatographic challenge.</p><h4><strong>Why Attend?</strong></h4><ul><li>Improve method robustness with a structured development strategy</li><li>Reduce guesswork and accelerate column selection decisions</li><li>Compare multiple column chemistries for challenging separations</li><li>Understand how system dispersion impacts chromatographic performance</li><li>Apply a proven workflow to your next LC method challenge</li></ul><blockquote><p><strong>Speaker: </strong>Tobias Langrock (Senior Application Specialist Chemistry, Europe, Waters Corporation)</p><p>During his time as a PhD student Tobias discovered his passion for LC and LC-MS/MS and has not lost it since. Joining Waters in 2008 was the perfect opportunity to expand his understanding and expertise in chromatography and mass spectrometry. Since then, he has been involved with a very wide range of instruments and applications, whether small or large molecules, reversed phase LC, IEX or SEC, SFC or GPC. His focus is always on the customers and their daily chromatographic challenges.</p></blockquote>

Creating Better Chromatographic Methods Using a Systematic Approach

Master robust LC method development. Discover a systematic tiered workflow to screen column chemistries and optimize challenging steroid separations

Creating Better Chromatographic Methods

<h4><strong>DMPK workflows, not workarounds</strong></h4><p>Current LC-MS/MS platforms can routinely achieve sub ng/mL levels of sensitivity for large molecule bioanalysis. However, as therapeutic modalities become more effective, or as the delivery mechanism changes from IV to oral, there is an increasing demand for bioanalytical workflows that can achieve even lower levels of sensitivity.</p><p>Join us to learn how moving to microflow can allow you to improve your limits of quantification, and unlock the full potential of the complete, end-to-end bioanalytical workflow.</p><h5><strong>Topics we’ll explore</strong></h5><ul><li>Advantages of using microflow to improve sensitivity for large molecule bioanalysis</li><li>Key learnings for deploying microflow LC-MS/MS in routine bioanalytical environments</li><li>How using microflow can help reduce solvent usage, increase cost savings and reduce environmental footprint</li></ul><h5><strong>Who should attend</strong></h5><p>This webinar is designed for Research Associates, Scientists, Directors, Lab managers in pharma, biopharma, biotech and contract research organizations performing bioanalysis.</p><blockquote><p><strong>Speaker: </strong>Dr. Robert Plumb (Scientific Advisor, DMPK, Pharma Quan Program, Waters Corporation)</p><p>Dr Plumb is currently a scientific advisor in the LCMS Business Unit at Waters Corporation. &nbsp;He has several years of experience in the pharmaceutical industry beginning in 1987 at Glaxo R&amp;D DMPK Department focusing on regulatory bioanalysis and metabolite identification.</p><p>In 2001 he moved to Waters Corporation in Milford, MA, USA where he has worked in R&amp;D, pharmaceutical business development, metabolic phenotyping and discovery omics.</p><p>Research interest includes application of fast chromatography and advanced mass spectrometry in bioanalysis and omics studies. Dr Plumb has published over 150 papers on LC/MS(MS) and NMR for bioanalysis, metabolomics and metabolite identification.</p></blockquote><blockquote><p><strong>Speaker: </strong>Nikunj Tanna (Pharma Marketing Manager, Waters Corporation)</p><p>Nikunj Tanna is the Pharma Marketing Manager at Waters corporation. His role at Waters focuses on complete workflow solutions for quantitation of small and large molecules.</p><p>These end-to-end workflows include automation, novel sample preparation and chromatographic separation approaches, followed by detection using TQ platforms.</p></blockquote>

Lower the Flow to see even more

Maximize large molecule bioanalysis sensitivity. Discover how microflow LC-MS/MS enhances limits of quantification and lowers laboratory solvent costs

Lower the Flow to See More: Microflow LC-MS/MS Benefits

<p><strong>Whether you’re starting a new method or running a routine one, having a checklist to review before starting can help avoid chromatography problems.</strong></p><h4><strong>In this webinar, we will discuss:</strong></h4><ul><li>Sample characteristics (target analytes, matrix, etc.) and how to prepare the sample for LC analysis</li><li>Which supplies to use to get the best results possible</li><li>How to select the appropriate column, equilibrate it, benchmark it with your system, and how to take care of it</li><li>Getting to know your LC system, the importance of maintenance and having a “housekeeping” list.</li></ul><blockquote><p><strong>Speaker:</strong> Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)</p><p>Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.</p><p>Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.</p><p>Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.</p></blockquote>

Agilent Technologies: Becoming A Better Chromatographer Webinar Series

Preparing for LC Analysis: What to Consider Ahead of Time

Master LC analysis preparation. Discover essential checklists for sample matrix prep, column benchmarking, and routine liquid chromatography maintenance

Preparing for LC Analysis: Checklists and Maintenance

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Analytical laboratories face a challenging reality: Lower regulatory limits demand higher sensitivity, yet complex sample matrices can compromise data precision and instrument uptime. Shimadzu's latest LCMS-8065XE addresses this challenge with advanced ion source design, optimised ion transmission, and ultra-fast polarity switching technology.</strong></p><p>In this webinar, we will analyze real-world performance data demonstrating how LCMS-8065XE enables high sensitivity analysis even in complex matrices. You will see how to maintain exceptional quantitative precision during rapid chromatographic runs across environmental, pharmaceutical, and food safety workflows.</p><h4><strong>Learning Objectives</strong></h4><ul><li>Explore the engineering innovations behind the LCMS-8065XE that deliver a new benchmark in triple quadrupole sensitivity and ion transmission</li><li>Learn how improved sensitivity and signal stability can be translated into ROI - maximize daily throughput, reduced downtime, and increase overall data confidence</li><li>See how the LCMS-8065XE seamlessly integrates into a complete, high-efficiency LCMS workflow</li></ul><blockquote><p><strong>Presenter: </strong>Yap Siew Qi (Senior Product Specialist (LCMS), Shimadzu (Asia Pacific), Pte Ltd</p></blockquote></div></div></div>

Shimadzu: Meet LCMS-8065XE: Achieving High-Throughput Ultra-Trace Analysis 

Meet LCMS-8065XE: Achieving High-Throughput Ultra-Trace Analysis

we will analyze real-world performance data demonstrating how LCMS-8065XE enables high sensitivity analysis even in complex matrices.

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