<p><strong>🎤Amanda Guiraldelli Mahr&nbsp;</strong></p><p><strong>Amanda shares insights from her work across small molecules, peptides, proteins, and biopharmaceuticals, including her experience with LC-MS, metabolomics, impurity analysis, and oligonucleotide therapeutics.</strong></p><h4><strong>What You’ll Learn in This Episode:</strong></h4><ul><li>What AQbD actually means (in simple terms)</li><li>How DOE differs from traditional one-factor-at-a-time experiments</li><li>Why modern labs are shifting toward predictive, data-driven method development</li><li>The biggest benefits of DOE: more insight with fewer experiments*</li><li>Real-world example: optimizing oligonucleotide impurity separations using AQbD&nbsp;</li><li>Key LC parameters that impact performance (pH, gradient, column, temperature)</li><li>Challenges of working with large molecules vs. small molecules</li><li>Tools and software for DOE and chromatographic modeling</li><li>How AI and modeling are shaping the future of chromatography</li></ul><blockquote><p><strong>Key Takeaway: </strong>“Shift your mindset from testing to understanding.”</p></blockquote><h4><strong>Who This Episode Is For:</strong></h4><ul><li>Undergraduate and graduate chemistry students</li><li>Analytical scientists and method developers</li><li>Pharma &amp; biopharma professionals</li><li>Anyone working with LC, LC-MS, or chromatography method development</li></ul><h3><strong>Video Transcription</strong></h3><p>In this episode of <i>Concentrating on Chromatography</i>, David interviews <strong>Amanda Guiraldelli Mahr</strong>, an analytical chemist whose career has spanned pharmaceuticals, metabolomics, regulatory science, mass spectrometry, and biopharmaceutical analysis. Amanda discusses her journey from pharmacy and biochemistry studies to working with chromatography and mass spectrometry in academic, regulatory, and industrial environments.</p><p>The conversation focuses on the growing role of <strong>Analytical Quality by Design (AQbD)</strong>, <strong>Design of Experiments (DOE)</strong>, predictive modeling, and the future integration of artificial intelligence into chromatographic method development. Amanda also shares practical insights into method development for small molecules, peptides, proteins, oligonucleotides, and emerging therapeutics.</p><h4><strong>Early Career and Introduction to Chromatography</strong></h4><p>Amanda's interest in analytical chemistry began during her studies in pharmacy and biochemistry. An internship focused on chromatography for botanical medicine analysis introduced her to separation science and inspired her to pursue a PhD in analytical chemistry.</p><p>During her doctoral research, she worked extensively with <strong>LC-MS-based metabolomics</strong>, which led her to explore experimental design approaches and method development strategies. Her passion for chromatography and mass spectrometry deepened further while working at the United States Pharmacopeia (USP), where she routinely applied chromatographic and mass spectrometric techniques to characterize:</p><ul><li>Small-molecule drug substances</li><li>Pharmaceutical ingredients</li><li>Dietary supplements</li><li>Botanical products</li><li>Peptides</li><li>Proteins</li></ul><p>Her work increasingly involved understanding chromatographic separation mechanisms through experimental design and modeling approaches.</p><h4><strong>Expanding into Proteomics, Nitrosamines, and Biopharmaceuticals</strong></h4><p>Amanda's scientific curiosity led her beyond traditional small-molecule analysis.</p><p>Throughout her career she gained experience in:</p><ul><li>High-resolution mass spectrometry</li><li>Structural elucidation</li><li>Proteomics</li><li>Bioanalysis</li><li>Trace impurity analysis</li><li>Nitrosamine monitoring</li><li>Oligonucleotide therapeutics</li><li>Large proteins and peptides</li></ul><p>She worked with advanced fragmentation techniques including:</p><ul><li>CID (Collision-Induced Dissociation)</li><li>HCD (Higher-Energy Collisional Dissociation)</li><li>UV Photodissociation</li></ul><p>Later, she became involved in developing highly sensitive LC-MS and GC-MS methods for monitoring <strong>nitrosamines</strong>, a major regulatory concern due to their genotoxic potential. She also trained regulatory agencies in mass spectrometry applications and method validation before moving into biopharmaceutical research and contract research activities focused on proteins, peptides, oligonucleotides, and RNA therapeutics.</p><h4><strong>What Does a Chromatographer Do Every Day?</strong></h4><p>When asked to summarize the day-to-day work of a chromatographer, Amanda highlighted three core activities:</p><ul><li>Designing experiments</li><li>Troubleshooting analytical methods</li><li>Interpreting unexpected results</li></ul><p>She emphasized that a large portion of her work involves data analysis and learning from experimental outcomes in order to optimize methods and better understand chromatographic behavior.</p><h4><strong>Understanding Analytical Quality by Design (AQbD)</strong></h4><p>One of the central topics of the discussion was <strong>Analytical Quality by Design (AQbD)</strong>.</p><p>Amanda describes AQbD as a systematic approach for understanding how analytical methods perform and identifying factors that influence method variability.</p><p>Key objectives of AQbD include:</p><ul><li>Identifying critical method parameters</li><li>Understanding sources of variability</li><li>Building robustness into methods from the beginning</li><li>Improving method reliability throughout its lifecycle</li></ul><p>Rather than correcting problems after implementation, AQbD focuses on proactively designing analytical procedures that consistently deliver high-quality results.</p><p>Amanda noted that AQbD principles are strongly linked to pharmaceutical quality frameworks established through ICH guidelines such as:</p><ul><li>ICH Q8 (Pharmaceutical Development)</li><li>ICH Q14 (Analytical Procedure Development)</li></ul><p>These guidelines have significantly influenced modern pharmaceutical method development practices.</p><h4><strong>AQbD vs. Traditional Trial-and-Error Development</strong></h4><p>Amanda contrasted AQbD with the traditional <strong>one-factor-at-a-time (OFAT)</strong> approach.</p><h5><strong>Traditional Approach</strong></h5><ul><li>Changes one parameter at a time</li><li>Provides limited understanding of interactions</li><li>Often misses important relationships between variables</li></ul><h5><strong>AQbD Approach</strong></h5><ul><li>Uses multivariate experimental designs</li><li>Studies multiple variables simultaneously</li><li>Reveals interactions between parameters</li><li>Enables predictive modeling</li><li>Defines robust operating ranges</li></ul><p>According to Amanda, AQbD provides significantly more knowledge about method behavior while reducing unnecessary experimentation.</p><h4><strong>Design of Experiments (DOE): A Core AQbD Tool</strong></h4><p>Amanda clarified an important misconception:</p><p><strong>DOE is not the same as AQbD.</strong></p><p>Instead:</p><ul><li>AQbD is a broader philosophy and lifecycle approach.</li><li>DOE is one of the most powerful tools used within AQbD.</li></ul><p>DOE helps scientists:</p><ul><li>Identify critical factors</li><li>Evaluate interactions</li><li>Map regions of good and poor performance</li><li>Support risk assessment</li><li>Build predictive models</li></ul><p>However, AQbD also includes risk management, control strategies, and lifecycle monitoring beyond experimental design itself.</p><h4><strong>Practical Advice for Scientists New to DOE</strong></h4><p>For scientists interested in implementing DOE, Amanda recommends starting with simple screening studies.</p><p>Examples include evaluating:</p><ul><li>LC column chemistry</li><li>Mobile-phase pH</li><li>Organic modifiers</li><li>Temperature</li></ul><p>Important responses to monitor may include:</p><ul><li>Resolution</li><li>Peak tailing</li><li>Selectivity</li><li>Efficiency</li></ul><p>She encourages scientists to focus not only on collecting data but on understanding the patterns revealed by experimental results. As experience grows, more advanced optimization studies and predictive models can be developed.</p><h4><strong>Which LC Parameters Matter Most?</strong></h4><p>Amanda emphasized that critical parameters depend on:</p><ul><li>Sample type</li><li>Separation mode</li><li>Intended method purpose</li><li>Development stage</li></ul><p>For reversed-phase LC, common screening parameters include:</p><ul><li>Column chemistry</li><li>Mobile-phase pH</li><li>Organic solvent composition</li><li>Temperature</li></ul><p>For ion-pair chromatography, additional factors such as:</p><ul><li>Ion-pairing reagent type</li><li>Ion-pairing reagent concentration</li></ul><p>can strongly influence selectivity and retention behavior.</p><p>She stresses that proper risk assessment should always guide parameter selection.</p><h4><strong>Case Study: Oligonucleotide Impurity Profiling</strong></h4><p>Amanda shared an example involving impurity analysis of <strong>oligonucleotide therapeutics</strong>.</p><p>One major challenge is that impurities are often structurally very similar to the active pharmaceutical ingredient (API), making chromatographic separation difficult.</p><p>Using AQbD and DOE, her team systematically evaluated:</p><ul><li>LC column chemistries</li><li>Ion-pairing reagents</li><li>Temperature</li><li>Gradient conditions</li></ul><p>A desirability-function approach allowed simultaneous optimization of multiple critical resolutions rather than focusing on a single impurity pair.</p><p>This strategy enabled the development of an LC-UV method capable of separating impurities that might otherwise require LC-MS for quantification.</p><h4><strong>The Challenges of Large Molecules</strong></h4><p>Amanda identifies large biomolecules as some of the most challenging analytes in chromatography.</p><p>Compared with small molecules, proteins and oligonucleotides exhibit:</p><ul><li>Slower mass transfer kinetics</li><li>Peak broadening</li><li>Reduced sensitivity</li><li>Greater sensitivity to system-related factors</li></ul><p>Even seemingly minor differences can affect performance, including:</p><ul><li>Dwell volume</li><li>Tubing dimensions</li><li>System dispersion</li></ul><p>She also highlighted non-specific adsorption as a major challenge.</p><p>Examples include:</p><h5><strong>Oligonucleotides</strong></h5><p>Negatively charged phosphate backbones can interact strongly with metal surfaces, reducing recovery and reproducibility.</p><h5><strong>Peptides</strong></h5><p>Basic amino acid residues may interact with residual silanol groups on silica-based stationary phases, affecting chromatographic performance.</p><p>These challenges have driven innovation in:</p><ul><li>Low-adsorption LC systems</li><li>Surface-modified columns</li><li>Hybrid particle technologies</li><li>Improved consumables and hardware design</li></ul><h4><strong>Software and Modeling Tools</strong></h4><p>Amanda discussed several software platforms commonly used for DOE and method optimization:</p><h5><strong>DOE Software</strong></h5><ul><li>Design-Expert</li><li>JMP</li><li>Minitab</li><li>MODDE</li></ul><h5><strong>Chromatographic Modeling Software</strong></h5><ul><li>DryLab</li><li>ACD/AutoChrom</li><li>ChromSword</li></ul><p>She also noted that modern software increasingly integrates directly with chromatographic data systems, enabling automated experiment execution and data analysis.</p><h4><strong>Essential Skills Beyond Instrument Operation</strong></h4><p>According to Amanda, successful chromatographers need more than technical instrument expertise.</p><p>Key skills include:</p><ul><li>Chromatographic fundamentals</li><li>Statistics</li><li>Chemometrics</li><li>Data interpretation</li><li>Predictive modeling</li><li>Risk assessment</li></ul><p>She emphasized that statistical literacy is particularly important for implementing AQbD principles and validating predictive models.</p><h4><strong>The Future of Chromatography</strong></h4><p>Looking ahead, Amanda is particularly excited about the integration of:</p><ul><li>Artificial intelligence</li><li>Predictive modeling</li><li>Knowledge-driven method development</li></ul><p>She believes these technologies will help scientists better understand separation mechanisms and accelerate development of methods for increasingly complex therapeutics.</p><p>Ultimately, she expects analytical science to play an even more central role in pharmaceutical development, manufacturing, and quality assurance.</p><h4><strong>Conclusion</strong></h4><p>Amanda's career illustrates how chromatography has evolved from traditional small-molecule analysis into a highly interdisciplinary field spanning proteomics, biopharmaceuticals, oligonucleotide therapeutics, and advanced regulatory science. Her experience demonstrates the value of combining chromatographic expertise with AQbD principles, DOE, risk assessment, and predictive modeling.</p><p>As analytical challenges continue to grow in complexity, approaches that emphasize understanding, prediction, and quality by design are becoming increasingly important. The future of chromatography is likely to be shaped by data-driven decision making, advanced modeling, and artificial intelligence—tools that will enable scientists to develop more robust, efficient, and reliable analytical methods.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

AQbD Explained: How Design of Experiments is Transforming Chromatography

Untargeted Metabolomics Identifies N-Lactoyl-Amino Acids as Dose-Responsive Plasma Biomarkers of Metformin Adherence in Type 2 Diabetes

<p><strong>In the week of June 22, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Simplifying Complexity in GLP-1 Receptor Agonist Analysis: From Structural Characterization to Impurity Monitoring</strong></h5><ul><li>Tu, 23.6.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6275">Registration</a></li></ul><p>Simplify GLP-1 receptor agonist analysis. Learn how a streamlined LC-MS workflow combines UHPLC and high-resolution MS for peptide characterization and impurity monitoring.</p><h5><strong>2. Tosoh Bioscience: Tosoh Tuesday - Exploring the AAV Toolkit</strong></h5><ul><li>Tu, 23.6.2026 15:45 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6273">Registration</a></li></ul><p>Improve AAV analysis and purification. Learn how to increase recovery and purity using the TOYOPEARL GigaCap Q-650S resin and columns from Tosoh Bioscience.</p><h5><strong>3. Phenomenex: Sustainable Strategies for High Sensitivity Analysis for Clinical Research LC-MS/MS Methods</strong></h5><ul><li>We, 24.6.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6278">Registration</a></li></ul><p>Optimize clinical LC-MS/MS methods using sustainable ethyl acetate SLE to extract steroids, MMA, and estrogens from human serum with high recovery.</p><h5><strong>4. Agilent Technologies: Preparing for LC Analysis: What to Consider Ahead of Time</strong></h5><ul><li>We, 24.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6288">Registration</a></li></ul><p>Avoid chromatography problems before they start. Learn how to prepare sample matrices, select and maintain LC columns, and establish an effective system housekeeping list.</p><h5><strong>5. SelectScience: Next-generation metabolomics: From annotation to discovery at scale</strong></h5><ul><li>We, 24.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6281">Registration</a></li></ul><p>Explore how next-generation mass spectrometry, ion mobility, and advanced computational tools accelerate confident small-molecule annotation and discovery in metabolomics.</p><h5><strong>6. Waters Corporation: Clear the Noise: Improving Resolution of GLP-1 Receptor Agonist and Insulin Impurities</strong></h5><ul><li>We, 24.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6262">Registration</a></li></ul><p>Learn how to gain greater resolution for your GLP-1 RA and insulin impurity analysis separations.</p><h5><strong>7. Shimadzu Corporation: Meet LCMS-8065XE: Achieving High-Throughput Ultra-Trace Analysis</strong></h5><ul><li>Th, 25.6.2026 08:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6290">Registration</a></li></ul><p>In this webinar, we will analyze real-world performance data demonstrating how LCMS-8065XE enables high sensitivity analysis even in complex matrices.</p><h5><strong>8. Waters Corporation: Creating Better Chromatographic Methods Using a Systematic Approach</strong></h5><ul><li>Th, 25.6.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6284">Registration</a></li></ul><p>Stop guessing and start separating. Learn a structured, systematic approach to LC method development to improve robustness and accelerate column selection.</p><h5><strong>9. Waters Corporation: Lower the Flow to see even more</strong></h5><ul><li>Th, 25.6.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6285">Registration</a></li></ul><p>Boost large molecule sensitivity in bioanalysis. Learn how switching to microflow LC-MS/MS improves limits of quantification, cuts costs, and reduces solvent usage.</p><h5><strong>10. Separation Science: Advancing PFAS Analysis: From Hidden Precursors to Food Safety Workflows</strong></h5><ul><li>Th, 25.6.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6286">Registration</a></li></ul><p>Discover practical approaches for detecting, analyzing, and addressing PFAS contamination in environmental and food-related samples.</p><h5><strong>11. Waters Corporation: Achieving Accurate BOD Results: What Your Questions Revealed</strong></h5><ul><li>Th, 25.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6283">Registration</a></li></ul><p>Get consistent and defensible BOD results by learning how to troubleshoot common testing errors and standardize your laboratory procedures.</p><h5><strong>12. SelectScience: Future-ready biobanking starts with smarter sample storage</strong></h5><ul><li>Fr, 26.6.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6289">Registration</a></li></ul><p>Safeguard your specimens in biobanking and clinical research. Learn from Thermo Fisher Scientific experts how to optimize sample storage, traceability, and compliance.</p><h5><strong>13. SelectScience: Enhancing Empower software data with cloud-based applications</strong></h5><ul><li>Fr, 26.6.2026 17:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6287">Registration</a></li></ul><p>Connect your chromatography data with Waters’ new cloud-based software solutions. Learn how to track system status in real time and enable AI-powered data analytics.</p>

Webinars LabRulezLCMS Week 26/2026

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Webinars

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Scientists

Scientific article

Interview

Science and research

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Tosoh Bioscience

Separation Science

Agilent Technologies

Phenomenex

Thermo Fisher Scientific

Shimadzu Corporation

Waters Corporation

<p><strong>In the race to launch GLP‑1 generics and novel agonists, complex impurity analyses directly impact your speed to market. Conventional columns often obscure peaks of critical impurities, leading to costly rework and increased regulatory scrutiny.</strong></p><p>Join us and learn about new analytical tools designed to streamline your process and deliver reproducible, high-resolution separations of GLP‑1 receptor agonists (GLP-1 RAs) and insulin-related impurities without relying on suppressive mobile‑phase additives.</p><p>Learn how improved resolution can accelerate method development, increase confidence in your results, and support faster time to market for your GLP-1 RA and insulin products.</p><h4><strong>In this webinar you will learn:</strong></h4><ul><li>How to gain greater resolution for your GLP-1 RA and insulin impurity analysis separations</li><li>How to achieve up to 3x faster separations</li><li>How to identify subtle impurities, such as single amino-acid substitutions and deamidation, beyond the detection limits of conventional reversed-phase technologies</li></ul><h4><strong>Who should attend</strong></h4><p><strong>This webinar is designed for you if you work in:</strong></p><ul><li>Analytical development</li><li>Process development</li><li>R&amp;D</li><li>CXOs dealing with GLP-1 RAs or insulin</li></ul><blockquote><p><strong>Presenter: </strong>Michael Zagieboylo (Principal Product Manager, Waters Corporation)</p><p>Michael Zagieboylo is a Principal Product Manager at Waters Corporation whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of biological molecules, focusing on peptide and protein separations.</p></blockquote><blockquote><p><strong>Presenter: </strong>Steve Shiner (Senior Manager, R&amp;D for Biopharma Solutions, Waters Corporation)</p></blockquote>

Clear the Noise: Improving Resolution of GLP-1 Receptor Agonist and Insulin Impurities

Resolve GLP-1 receptor agonist and insulin impurities faster. Discover analytical tools for high-resolution separation of deamidation and amino-acid changes.

Improving Resolution of GLP-1 and Insulin Impurities

<p><strong>On June 23, Abbie Breton, Applications Development Manager, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts. Yuliya McAnany, Applications Scientist – Process Resins at Tosoh Bioscience, will showcase a suite of high-performance tools for reliable separation and analysis of AAV particles. Yuliya will start by discussing Tosoh’s novel full capsid enrichment method for TOYOPEARL GigaCap Q-650S resin combined with choline chloride, achieving 90% purity and 75% recovery. The conversation will include a look into Tosoh’s analytical toolkit for increased reliability, and improved sensitivity for critical quality monitoring.</strong></p><p>Participants will learn how to simplify the development of a high-performing enrichment step and will learn how Tosoh’s TSKgel Q-STAT, TSKgel GMPWxl, and LenS3 MALS detector can be used to advance quality monitoring of AAV products.</p><p>After the webinar, ask your questions during the live Q&amp;A session.&nbsp;</p><blockquote><p><strong>Presenter:</strong> Abbie Breton (Applications Development Manager, Tosoh Bioscience)</p></blockquote><blockquote><p><strong>Presenter:</strong> Yuliya McAnany (Applications Scientist – Process Resins, Tosoh Bioscience)</p></blockquote>

Tosoh Bioscience: Tosoh Tuesday - Exploring the AAV Toolkit

Tosoh Tuesday - Exploring the AAV Toolkit

Advance your AAV quality monitoring. Explore Tosoh's full capsid enrichment methods using TOYOPEARL resins and precise TSKgel SEC analysis with MALS detection

Exploring the AAV Toolkit: Tosoh Chromatography Webinar

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>GLP-1 receptor agonists are among the most impactful peptide therapeutics, but their structural complexity presents significant analytical challenges. From sequence modifications to lipidation and manufacturing variability, achieving confident characterization requires advanced approaches.</strong></p><p>Join us to learn how a streamlined LC-MS workflow can simplify GLP-1 analysis. This session will highlight integrated strategies combining UHPLC separation, high-resolution mass spectrometry, and automated data analysis to support robust characterization and impurity monitoring of peptides such as semaglutide and tirzepatide.</p><h5><strong>What You Will Learn</strong></h5><ul><li>How to optimize LC-MS workflows for comprehensive GLP-1 peptide characterization</li><li>Practical strategies for improving separation of closely related peptide species</li><li>Approaches to enhance sequence coverage using advanced fragmentation techniques</li><li>Methods for confident impurity profiling and structural heterogeneity assessment</li></ul><p>Secure your spot today and gain practical insights to simplify complex GLP-1 analysis.</p><blockquote><p><strong>Presenter: </strong>Dr. Sara Carillo (Bioanalytical Research Lead, NIBRT)</p><p>Dr. Carillo specializes in structural characterization of complex biomolecules using mass spectrometry and NMR. At NIBRT, she develops advanced analytical strategies to better understand biomanufacturing processes and the structural complexity of biopharmaceuticals.</p></blockquote><blockquote><p><strong>Presenter: </strong>Dr. Kai Sheffler (Senior Manager, Product Manager for BioPharma Workflows, Thermo Fisher Scientific)</p><p>Dr. Scheffler is a biochemist specializing in LC-MS technologies for biopharmaceutical analysis. His expertise includes peptide mapping, intact mass analysis, and impurity profiling using HRAM MS. He focuses on developing LC-MS solutions for comprehensive characterization and quality assessment of biopharmaceuticals.</p></blockquote><blockquote><p><strong>Moderator:</strong> Dr. Kelly Broster (Sr Manager, Market Development &amp;&nbsp;Collaborations, Pharma Biopharma, Thermo Fisher Scientific)</p></blockquote></div></div></div>

Thermo Fisher Scientific: Simplifying Complexity in GLP-1 Receptor Agonist Analysis: From Structural Characterization to Impurity Monitoring

Simplifying Complexity in GLP-1 Receptor Agonist Analysis: From Structural Characterization to Impurity Monitoring

Optimize LC-MS workflows for GLP-1 analysis. Master impurity profiling, peptide separation, and structural characterization of semaglutide and tirzepatide

Simplifying GLP-1 Agonist Analysis & Impurity MS

<h4><strong>One webinar, two viewing options</strong></h4><p><strong>We know you're busy, select a viewing session that works best for you.</strong></p><h5><br><strong>Viewing Options</strong></h5><p><strong>Session 1</strong></p><ul><li>Tuesday, June 23, 2026</li><li>Los Angeles: 10:00 AM PT | New York: 1:00 PM EST</li><li>Mexico City: 12:00 PM CDT</li></ul><p><strong>Session 2</strong></p><ul><li>Wednesday, June 24, 2026</li><li>London: 9:00 AM BST | Berlin: 10:00 AM CEST&nbsp;</li><li>Mumbai: 1:30 PM IST</li></ul><p>Supported Liquid Extraction (SLE) for the cleanup of biological matrices has several advantages over Solid Phase Extraction (SPE). SLE requires fewer steps and less method development compared to SPE. For most samples that can be processed by liquid-liquid extraction, SLE can usually be used to provide cleaner and faster solvent partitioning. Historically, to get the cleanest SLE extracts, halogenated solvents like dichloromethane were used for elution of target analytes from the sorbent. A supported liquid extraction (SLE) approach that produced clean extracts with high recoveries and low matrix interferences using ethyl acetate, a more sustainable organic solvent, will be presented. Results for steroids, methylmalonic acid (MMA), and underivatized estrogens in human serum, in addition to optimized LC-MS/MS conditions and LC column considerations, are also discussed.</p><h4><strong>Key Learning Points</strong></h4><ul><li>Discover how SLE provides clean extracts for LC-MS/MS quantitation at low concentrations.</li><li>Describe conditions for the extraction and analysis of MMA from human serum.</li><li>Define method parameters for low-level quantitation of estrogens without derivatization.</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>All clinical, CROs, and forensic/toxicology analysts using LC/MS-MS.</li></ul><blockquote><p><strong>Presenter: </strong>Stephanie J Marin (Sr. Global Market Development Manager - Applied Markets, Phenomenex)</p><p>Stephanie J. Marin is the Clinical and Forensics Global Market Development Manager at Phenomenex. She received her Ph.D. in chemistry from Arizona State University and has expertise in sample preparation, liquid chromatography, and mass spectrometry. She has over 10 years of experience developing and validating clinical methods from her tenure at the ARUP Institute for Clinical and Experimental Pathology. Dr Marin has worked in marketing, product development, and applications development, in addition to customer-facing roles in customer and technical support, service, and customer education and training. She has also held positions at Hamilton Company, Selerity Technologies, and Biotage, and in analytical services for specialty chemicals and polymers at Rohm and Haas (now Dow). She was also a supervisor at an EPA-certified laboratory. She is the author of over 30 peer-reviewed publications, book chapters, and over 100 abstracts presented at national and international meetings.&nbsp;</p></blockquote>

Phenomenex: Sustainable Strategies for High Sensitivity Analysis for Clinical Research LC-MS/MS Methods 

Sustainable Strategies for High Sensitivity Analysis for Clinical Research LC-MS/MS Methods

Advance clinical LC-MS/MS analysis. Discover sustainable Supported Liquid Extraction (SLE) methods for steroids, MMA, and underivatized estrogens in serum

Sustainable SLE and LC-MS/MS for Clinical Analysis

<h4><strong>Real‑World Secrets to Consistent, Defensible Results</strong></h4><p><strong>This webinar focuses on translating real laboratory challenges into practical strategies for improving BOD accuracy, consistency, and data defensibility. By addressing common issues, it helps labs understand why results vary and how to troubleshoot them effectively.</strong></p><p>Biochemical Oxygen Demand (BOD) testing is a cornerstone of water quality analysis, yet many laboratories continue to experience variability and uncertainty in day to day execution. This presentation takes a practical, insight driven approach by focusing entirely on real questions submitted by laboratories during a previous session.</p><p>Rather than presenting a traditional step by step review of Standard Methods, this session explores recurring challenges that impact BOD accuracy, consistency, and defensibility. Topics are structured around common pain points identified across labs, including dilution water preparation, seed selection and performance, GGA recovery interpretation, dissolved oxygen (DO) measurement and calibration, and appropriate dilution and bottle selection.</p><p>Through these real-world scenarios, attendees will gain a deeper understanding of why BOD results vary, how biological factors influence outcomes, and how seemingly minor procedural differences can significantly affect data quality.</p><h5><strong>Key takeaways</strong></h5><ul><li>BOD variability is expected - control it through consistency</li><li>Preparation (water, seed, handling) drives results</li><li>GGA validates system performance, not samples</li></ul><p><strong>Who should attend</strong></p><p>This webinar is designed for Analytical scientists, Laboratory managers and Quality assurance managers.</p><p>Don't miss it!</p><blockquote><p><strong>Speaker:&nbsp;</strong>Christy Abbas, PhD (Sr. Product Marketing Manager, Waters ERA)</p><p>Christy has been with Waters ERA for six and a half years. Her career spans roles at an instrument manufacturer, a biopharmaceutical laboratory, and a mining company. She earned her PhD in Applied Chemistry from the Colorado School of Mines.</p><p>Throughout her career, Christy has supported the nuclear, pharmaceutical, and chemical industries, as well as academia. Drawing on her background in analytical chemistry, product development, marketing, and sales, she is focused on expanding Waters ERA’s product portfolio to better meet evolving customer needs.</p></blockquote>

Achieving Accurate BOD Results: What Your Questions Revealed

Improve your BOD analysis accuracy. Learn practical troubleshooting for dilution water, seed performance, and dissolved oxygen measurement workflows

Achieving Accurate BOD Results: Water Quality Analysis

<h3><strong>Stop Guessing, Start Separating - A Smarter Path to Robust LC Methods</strong></h3><h4><strong>Overview</strong></h4><p>Developing robust LC methods doesn’t need to rely on trial and error. In this webinar, we introduce a systematic, tiered approach to chromatographic method development - from verifying system health and understanding instrument dispersion to screening multiple column chemistries and optimizing for selectivity and robustness.</p><p>Through a real-world case study, we demonstrate how to separate a complex steroid mixture using six different column chemistries, including our new ACQUITY™ Biphenyl RP column, which delivers notable selectivity advantages for this challenging application.</p><p>Join us to see how a structured screening strategy can accelerate method development and help you make confident, data-driven decisions for your next chromatographic challenge.</p><h4><strong>Why Attend?</strong></h4><ul><li>Improve method robustness with a structured development strategy</li><li>Reduce guesswork and accelerate column selection decisions</li><li>Compare multiple column chemistries for challenging separations</li><li>Understand how system dispersion impacts chromatographic performance</li><li>Apply a proven workflow to your next LC method challenge</li></ul><blockquote><p><strong>Speaker: </strong>Tobias Langrock (Senior Application Specialist Chemistry, Europe, Waters Corporation)</p><p>During his time as a PhD student Tobias discovered his passion for LC and LC-MS/MS and has not lost it since. Joining Waters in 2008 was the perfect opportunity to expand his understanding and expertise in chromatography and mass spectrometry. Since then, he has been involved with a very wide range of instruments and applications, whether small or large molecules, reversed phase LC, IEX or SEC, SFC or GPC. His focus is always on the customers and their daily chromatographic challenges.</p></blockquote>

Creating Better Chromatographic Methods Using a Systematic Approach

Master robust LC method development. Discover a systematic tiered workflow to screen column chemistries and optimize challenging steroid separations

Creating Better Chromatographic Methods

<h4><strong>DMPK workflows, not workarounds</strong></h4><p>Current LC-MS/MS platforms can routinely achieve sub ng/mL levels of sensitivity for large molecule bioanalysis. However, as therapeutic modalities become more effective, or as the delivery mechanism changes from IV to oral, there is an increasing demand for bioanalytical workflows that can achieve even lower levels of sensitivity.</p><p>Join us to learn how moving to microflow can allow you to improve your limits of quantification, and unlock the full potential of the complete, end-to-end bioanalytical workflow.</p><h5><strong>Topics we’ll explore</strong></h5><ul><li>Advantages of using microflow to improve sensitivity for large molecule bioanalysis</li><li>Key learnings for deploying microflow LC-MS/MS in routine bioanalytical environments</li><li>How using microflow can help reduce solvent usage, increase cost savings and reduce environmental footprint</li></ul><h5><strong>Who should attend</strong></h5><p>This webinar is designed for Research Associates, Scientists, Directors, Lab managers in pharma, biopharma, biotech and contract research organizations performing bioanalysis.</p><blockquote><p><strong>Speaker: </strong>Dr. Robert Plumb (Scientific Advisor, DMPK, Pharma Quan Program, Waters Corporation)</p><p>Dr Plumb is currently a scientific advisor in the LCMS Business Unit at Waters Corporation. &nbsp;He has several years of experience in the pharmaceutical industry beginning in 1987 at Glaxo R&amp;D DMPK Department focusing on regulatory bioanalysis and metabolite identification.</p><p>In 2001 he moved to Waters Corporation in Milford, MA, USA where he has worked in R&amp;D, pharmaceutical business development, metabolic phenotyping and discovery omics.</p><p>Research interest includes application of fast chromatography and advanced mass spectrometry in bioanalysis and omics studies. Dr Plumb has published over 150 papers on LC/MS(MS) and NMR for bioanalysis, metabolomics and metabolite identification.</p></blockquote><blockquote><p><strong>Speaker: </strong>Nikunj Tanna (Pharma Marketing Manager, Waters Corporation)</p><p>Nikunj Tanna is the Pharma Marketing Manager at Waters corporation. His role at Waters focuses on complete workflow solutions for quantitation of small and large molecules.</p><p>These end-to-end workflows include automation, novel sample preparation and chromatographic separation approaches, followed by detection using TQ platforms.</p></blockquote>

Lower the Flow to see even more

Maximize large molecule bioanalysis sensitivity. Discover how microflow LC-MS/MS enhances limits of quantification and lowers laboratory solvent costs

Lower the Flow to See More: Microflow LC-MS/MS Benefits

<p><strong>By attending this webinar you will:</strong></p><ul><li>Gain practical approaches for detecting unknown PFAS in consumer goods and environmental samples</li><li>Understand a simplified LC-MS/MS workflow for sensitive, compliant PFAS analysis in animal-derived foods</li><li>Understand PFAS contamination risks in food and evolving regulatory expectations</li><li>Explore automated and semi-automated PFAS workflows for complex food matrices.</li></ul><h5><strong>Presentation 1: Hydrolysis as a Critically Important Step in the Analysis of Fluorotelomer Alcohols in Textiles and Other Products</strong></h5><ul><li>Vladimir Nikiforov, Senior Scientist, Environmental Chemistry and Health Effects, NILU, Tromsø</li></ul><p>In this webinar, a simple hydrolysis-based approach for identifying and accounting for PFAS precursors in consumer products and environmental samples will be presented by its creator. The theory behind the method will be explained. Advantages and limitations of the recently certified EN 17681-1:2025 method will be discussed. Hydrolysis will be compared to oxidation as an alternative/complementary work-up for release of perfluoroalkyl substances.</p><p>The presentation will focus on fluorotelomer alcohol (FTOH)-based precursor compounds, including polymeric materials that may degrade into regulated or hazardous PFAS. Attendees will learn how a straightforward hydrolysis procedure can convert precursor compounds into detectable free FTOHs while preserving valuable structural information for source identification and risk assessment. Case studies from textile extracts will demonstrate how preliminary hydrolysis revealed up to 1300-fold higher levels of previously “hidden” FTOHs compared to conventional analysis alone.</p><h5><strong>Presentation 2: PFAS Analysis in Animal‑Derived Foods Using a Direct Blend &amp; Inject LC‑MS/MS Workflow</strong></h5><ul><li>Phil Taylor, Marketing Lead – Food &amp; Environmental, PerkinElmer</li></ul><p>Per‑ and polyfluoroalkyl substances (PFAS) present major analytical challenges in animal‑derived foods due to complex matrices and tightening regulations. This presentation demonstrates a direct Blend &amp; Inject LC‑MS/MS workflow for quantifying 25 PFAS compounds aligned with EPA Method 533 and EU requirements. The approach delivers ppt‑level sensitivity, excellent linearity, and robust performance without solid‑phase extraction.</p><h5><strong>Presentation 3: PFAS in Food – Addressing Contamination in the Food Supply Chain</strong></h5><ul><li>Margot Lee, Senior Scientist, Waters Corporation &nbsp;and &nbsp;MJ Madden, Technical Support Manager and Project Manager, PromoChrom Technologies</li></ul><p>PFAS contamination in food presents a growing analytical and regulatory challenge, particularly as attention expands beyond water into complex food and animal protein matrices. In this session, Waters and PromoChrom experts explore practical, mostly automated workflows designed to improve robustness, efficiency, and data quality in PFAS food testing.</p><p>The presentation will feature real-world examples of PFAS analysis in food matrices, including eggs and animal proteins, highlighting different sample preparation strategies adapted to matrix complexity and laboratory throughput needs. Attendees will gain insight into how pressurized solvent extraction, SPE cleanup, and QuEChERS-based approaches can be combined with automation to streamline workflows while maintaining sensitivity and reproducibility.</p><p>As PFAS testing in food continues to evolve, this session will also provide a forward-looking view on emerging workflow optimizations and how labs can prepare for expanding regulations and increasing analytical demands.</p><h5><strong>What you need to know:</strong></h5><ul><li><strong>Duration:</strong> Approximately 90 minutes</li></ul><blockquote><p><strong>Presenter: </strong>Vladimir Nikiforov (Senior Scientist, Environmental Chemistry and Health Effects, NILU, Tromsø)</p><p>Vladimir Nikiforov is a senior scientist at NILU, working on method development for new types of analytes, with 40+ years experience in “PFAS” chemistry. He is a synthetic organic, organofluorine and physical organic chemist by background. In addition, he has 30+ years of experience in environmental chemistry with emphasis on trace organic analytical chemistry. Key expertise and research interest are: QSAR, PFAS, NTS, SVOC, suspect screening, microplastic, synthesis of standards, method development, GC, LC, MS, NMR, fate models, transformation products, reactivity of organic molecules, transformation kinetics, persistency, UV-compounds, tire additives, air sampling, air monitoring, Arctic pollution, derivatization methods, structure elucidation, international regulation, and chemical education. Previous publications and projects can be found here.</p></blockquote><blockquote><p><strong>Presenter:&nbsp;</strong>Phil Taylor (Marketing Lead – Food &amp; Environmental, PerkinElmer)</p><p><span>Phil Taylor is a Marketing Leader for Food &amp; Environmental markets at PerkinElmer, bringing nearly 20 years of experience across analytical chemistry, LC‑MS/MS applications, and science‑driven marketing. With a background spanning water analysis, food and environmental testing, and global LC‑MS/MS workflow launches, he specializes in translating complex analytical challenges into practical, regulation‑ready solutions for routine laboratories.</span></p></blockquote><blockquote><p><strong>Presenter:&nbsp;</strong>Margot Lee (Senior Scientist, Waters Corporation)</p><p>Margot Lee is a Senior Scientist at Waters Corporation, where she develops applications for PFAS (per- and polyfluoroalkyl substances) analysis using LC-MS/MS. She has worked at Waters since 2022 and has a background in biology with a career in analytical chemistry. After contributing to the validation of Waters’ dual-phase SPE cartridges for PFAS analysis, she joined the PFAS applications team in 2024. Her current work focuses on manual and automated extractions of PFAS in environmental and food matrices. She is passionate about advancing understanding of contaminants that impact public health and sharing practical methods that support the critical research of scientists around the world.</p></blockquote><blockquote><p><strong>Presenter: </strong>MJ Madden (Technical Support Manager and Project Manager, PromoChrom Technologies)</p><p>MJ Madden (They/She) earned a Master’s in Chemistry with honors from the University of British Columbia and joined PromoChrom Technology in 2023. As Technical Support Manager and Project Manager for new product development, MJ combines scientific expertise with a passion for customer success. They are currently leading initiatives to expand PromoChrom’s support services, including the launch of a comprehensive online support guide and enhanced field service capabilities for repairs and preventive maintenance. MJ brings a forward-looking perspective to environmental technology, helping customers adopt innovative solutions with confidence.<br>&nbsp;</p></blockquote>

Separation Science: Advancing PFAS Analysis - From Hidden Precursors to Food Safety Workflows

Advancing PFAS Analysis: From Hidden Precursors to Food Safety Workflows

Master PFAS contamination analysis. Discover hydrolysis for textils/precursors and automated direct Blend & Inject LC-MS/MS workflows for food matrices

Advancing PFAS Analysis: Hidden Precursors & Food Safety

<p><strong>Whether you’re starting a new method or running a routine one, having a checklist to review before starting can help avoid chromatography problems.</strong></p><h4><strong>In this webinar, we will discuss:</strong></h4><ul><li>Sample characteristics (target analytes, matrix, etc.) and how to prepare the sample for LC analysis</li><li>Which supplies to use to get the best results possible</li><li>How to select the appropriate column, equilibrate it, benchmark it with your system, and how to take care of it</li><li>Getting to know your LC system, the importance of maintenance and having a “housekeeping” list.</li></ul><blockquote><p><strong>Speaker:</strong> Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)</p><p>Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.</p><p>Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.</p><p>Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.</p></blockquote>

Agilent Technologies: Becoming A Better Chromatographer Webinar Series

Preparing for LC Analysis: What to Consider Ahead of Time

Master LC analysis preparation. Discover essential checklists for sample matrix prep, column benchmarking, and routine liquid chromatography maintenance

Preparing for LC Analysis: Checklists and Maintenance

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Analytical laboratories face a challenging reality: Lower regulatory limits demand higher sensitivity, yet complex sample matrices can compromise data precision and instrument uptime. Shimadzu's latest LCMS-8065XE addresses this challenge with advanced ion source design, optimised ion transmission, and ultra-fast polarity switching technology.</strong></p><p>In this webinar, we will analyze real-world performance data demonstrating how LCMS-8065XE enables high sensitivity analysis even in complex matrices. You will see how to maintain exceptional quantitative precision during rapid chromatographic runs across environmental, pharmaceutical, and food safety workflows.</p><h4><strong>Learning Objectives</strong></h4><ul><li>Explore the engineering innovations behind the LCMS-8065XE that deliver a new benchmark in triple quadrupole sensitivity and ion transmission</li><li>Learn how improved sensitivity and signal stability can be translated into ROI - maximize daily throughput, reduced downtime, and increase overall data confidence</li><li>See how the LCMS-8065XE seamlessly integrates into a complete, high-efficiency LCMS workflow</li></ul><blockquote><p><strong>Presenter: </strong>Yap Siew Qi (Senior Product Specialist (LCMS), Shimadzu (Asia Pacific), Pte Ltd</p></blockquote></div></div></div>

Shimadzu: Meet LCMS-8065XE: Achieving High-Throughput Ultra-Trace Analysis 

Meet LCMS-8065XE: Achieving High-Throughput Ultra-Trace Analysis

we will analyze real-world performance data demonstrating how LCMS-8065XE enables high sensitivity analysis even in complex matrices.

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