<p><strong>An international research team, which included scientists from the Faculty of Science at Palacký University and the Institute of Physics of the Czech Academy of Sciences, has succeeded in unraveling the complex mechanism by which a chemical bond is formed during a collision between molecules. The experts found that the reaction only takes place if the molecules are oriented towards each other within a very narrow range of angles – within the so-called cone of reaction. This joint research has thus provided an answer to one of the fundamental questions of chemistry: under what conditions do chemical bonds form between molecules. The results of the study&nbsp;</strong><i><strong>Spatially resolving the cone of reaction for a single molecule</strong></i><strong>&nbsp;were published in the prestigious scientific journal&nbsp;Science.</strong></p><p>Many chemical reactions begin with the collision of two particles. However, for a new chemical bond to form, it is not enough for the molecules simply to come into contact. Their collision must occur with sufficient energy, in the correct relative orientation and at the right location. Under normal conditions, such as in solutions or gases, molecules collide chaotically, and the precise parameters of these collisions cannot therefore be controlled.</p><h4><strong>Molecules under control</strong></h4><p>Scientists were able to control the collisions of individual molecules on a copper surface and monitored the process using a <strong>scanning tunnelling microscope</strong>. They used the instrument not only to visualise individual molecules, but also to deliberately accelerate the reactants towards one another. They subsequently analysed each collision with atomic-level resolution. This enabled them to determine unequivocally whether a new chemical bond had formed between the molecules.</p><h5><i><strong>“What is hidden in the statistics of billions of collisions in conventional chemistry, we have managed to break down into individual cases and map directly.” Adam Matěj</strong></i></h5><p>A key finding of the study is that the outcome of a collision is determined by the extremely precise orientation of the molecules. A new bond was formed only when the target molecule was oriented within a few degrees of the direction of movement of the other molecule. When rotated by more than fifteen degrees, the molecules simply bounced off each other without any reaction.</p><h4><strong>The precise orientation of the molecules is crucial</strong></h4><p><i>“For the first time, we were able to observe, using a single molecule, exactly how precisely it must be oriented for the reaction to take place at all. What is hidden in the statistics of billions of collisions in conventional chemistry, we have managed to break down into individual cases and map directly,” </i>said Adam Matěj from the Department of Physical Chemistry at the Faculty of Science.</p><p>The theoretical part of the study, in which researchers from<strong> Palacký University </strong>and <strong>the Institute of Physics of the Czech Academy of Sciences</strong> were involved, played a significant role. Using quantum-mechanical calculations, they explained why the ‘reaction cone’ is so narrow. It turned out that the reactive carbon atom is surrounded by hydrogen atoms, which block the access of the reacting molecule from almost all directions.</p><h4><strong>The arrangement of atoms restricts access to the reaction</strong></h4><p>The calculations also shed light on a surprising experimental result. The chemical reaction took place even when the molecule moved along an adjacent row of copper atoms, where, according to the original assumptions, no bond should have formed.</p><h5><i><strong>“The strength of this work lies in the fact that the experiment and theory have been brought together into a single picture. The microscope showed what happens during the collision, and the calculations explained why.” Pavel Jelínek</strong></i></h5><p><i>“The explanation lies in the slight displacement of a single copper atom on the surface. The target’s bond to the surface pulls it out of its position and brings it close enough to the neighbouring row that it can literally take over the projectile and direct it to the correct spot. This shows that the surface is not merely a passive substrate, but actively participates in the reaction itself and can even facilitate pathways that would otherwise be unfavourable,” </i>noted Adam Matěj.</p><h4><strong>The surface also determines the course of the reaction</strong></h4><p>The results are significant for the development of so-called surface reactions, in which new materials and nanostructures are purposefully assembled from individual molecules. A better understanding of the influence of the spatial arrangement of molecules will enable the design of molecular building blocks so that they bond more reliably and efficiently. This represents an important step towards the development of technologies based on the controlled construction of structures atom by atom.</p><p><i>“The strength of this work lies in the fact that experiment and theory have been brought together into a single picture. The microscope showed what happens during the collision, and the calculations explained why. Without a theoretical model, we would have found it difficult to discover that the movement of a single surface atom is behind the unexpected course of the reaction,” </i>added Pavel Jelínek from the Institute of Physics of the Czech Academy of Sciences.</p><blockquote><p>The study&nbsp;<i>Spatially resolving the cone of reaction for a single molecule</i>&nbsp;was produced in collaboration between researchers from the University of Graz, the Institute of Physics of the Czech Academy of Sciences, Palacký University in Olomouc, Charles University and Humboldt University in Berlin.</p></blockquote>

Scientists reveal when chemical bonds form in molecular collisions 

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 17th August 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you posters by <strong>Agilent Technologies / ASMS, Thermo Fisher Scientific / ASMS </strong>and<strong> Shimadzu / ASMS </strong>and application note by<strong> Waters Corporation!</strong></p><h3><strong>1. Agilent Technologies / ASMS: Quantitation of N-Nitroso Duloxetine N-Nitrosamine Drug Substance Related Impurities (NDSRI) in API and Formulation by 6475 LC/TQ</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_drug_impurities_lc_tq_tp_402_asms_2026_en_agilent_b933b9bddf.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Nitrosamine impurities are nitroso derivatives where the nitroso group is attached to an amine. The active pharmaceutical ingredient (API) derived from a class of nitrosamines are known as N-nitrosamine drugsubstance-related impurities (NDSRI). Nitrosamine impurities in APIs and drug products pose safety concerns, even in small quantities, and thus are a major concern for drug makers [1].&nbsp;</p><p>Analytical methods must be sufficiently sensitive to quantify the nitrosamine of interest at levels as low as 10% of the specification limit and, where feasible, down to ≤5% of the specification limit, for the determination of the limit of detection. LC/MS/MS is an inherently selective and highly sensitive analytical technique that is well suited for the identification and quantification of trace-level impurities at extremely low concentrations. Consequently, LC/MS/MS has been widely adopted within the pharmaceutical industry, particularly for nitrosamine analysis [2-3].&nbsp;</p><p>Therefore, manufacturers must develop and qualify a dedicated analytical method that is appropriate for the specific sample matrix and targeted NDSRIs. An LC/MS/MS method based on multiple reaction monitoring (MRM) was developed for the detection and quantification of the N-nitroso duloxetine NDSRI impurity over a concentration range of 0.5 to 40 ppb (absolute standard concentration), using an atmospheric pressure chemical ionization (APCI) source on an Agilent 6475A LC/TQ instrument.</p><h4><strong>Experimental</strong></h4><ul><li><strong>MS:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6475"><strong>6475 Triple Quadrupole LC/MS System</strong></a></li><li><strong>Column:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=Poroshell+HPH+C18"><strong>Poroshell HPH C18</strong></a> (4.6*150mm, 2.7µm)</li></ul><h4><strong>Conclusions</strong></h4><ul><li>A highly sensitive and robust MRM LC/MS/MS method was developed for the quantitation of N-nitroso duloxetine in duloxetine hydrochloride API and finished formulation using the Agilent 6475A LC/TQ.&nbsp;</li><li>The optimized chromatographic conditions provided effective separation of the analyte from the API, placebo, and formulation matrices, ensuring reliable quantitation.&nbsp;</li><li>The calibration curve was linear over the concentration range of 0.5-40 ng/mL, with 1/x weighting. R and R2 values were observed for both to be greater than 0.99.&nbsp;</li><li>Recovery studies demonstrated efficient sample extraction, with recoveries ranging from 92-99% in API samples and 109-116% in pellet formulations at the LOQ, specification, and 200% specification levels.&nbsp;</li><li>The method also showed excellent reproducibility across all evaluated concentration levels, confirming its suitability for routine analysis.</li></ul><h3><strong>2. Shimadzu / ASMS: Non-Targeted Analysis of Per- and Polyfluoroalkyl Substances in Environmental Extracts by Microflow LC-QToF Coupled with Multi-spray ESI</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/TP_562_Non_Targeted_Analysis_of_Per_and_Polyfluoroalkyl_Substances_in_Environmental_Extracts_by_Microflow_LC_Q_To_F_Coupled_with_Multi_spray_ESI_c2a7e1d5bf.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Targeted screening methods are well established for monitoring Perand polyfluoroalkyl substances (PFAS) in environmental samples. However, PFAS comprise a large and evolving class of compounds, creating a need to screen existing, emerging, and transformation products across diverse matrices. Non-targeted Analysis (NTA) allows for the confirmation of suspected PFAS in the environment, but the sample characterization is often limited by analytical sensitivity. Microflow (0.5 – 40 µL/min) coupled with a multi-nozzle emitter enhances sensitivity by improving desolvation. When this is coupled to a high mass accuracy and resolution Quadrupole Time-ofFlight Mass Spectrometer (QToF), capable of data-dependent and independent MS/MS, PFAS characterization of unknown analytes is possible. In this study, various environmental extracts were processed and screened using NTA for PFAS compounds.</p><h4><strong>Methods</strong></h4><p>A <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=Nexera+Mikros"><strong>Shimadzu Nexera Mikros LC</strong> </a>was used to introduce the sample to a Newomics DuoESI source with an M3 emitter on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=9050"><strong>LCMS-9050 QToF MS</strong></a> (Fig. 1). A direct injection on an octadecyl column without delay column was performed. Direct injection was chosen over trapand-elute configuration to minimize selectivity bias caused by the trap column.&nbsp;</p><h4><strong>Conclusion</strong></h4><p>With the increased sensitivity of microflow LC-QToF coupled with multi-spray ESI with DIA acquisition, Insight Explore aided in NTA PFAS analysis. When paired with Insight Discovery and Profiler, a streamlined batch data processing workflow with statistical analysis was shown to effectively handle complex environmental matrices and their analyses.</p><h3><strong>3. Thermo Fisher Scientific / ASMS: Robust and sensitive quantitation of 18 steroids in human serum using Thermo Scientific TSQ Certis Triple Quadrupole mass spectrometer</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004707_lsms_18_steroids_tsq_certis_clinical_po004707_asms2026_na_en_244c52437b.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Accurate quantification of endogenous steroids in human serum is essential for biomonitoring physiological functions such as hormone regulation, reproduction, and immune response in clinical research laboratories. Liquid chromatography–tandem mass spectrometry (LC–MS/MS) is the gold standard for steroid analysis due to its superior sensitivity, selectivity, and multiplexing capability compared to immunoassays. However, steroid measurements remain challenging because of low endogenous concentrations and complex serum matrices. Here, we describe a robust and sensitive LC–MS/MS method for the simultaneous quantification of 18 steroids in human serum using the Thermo Scientific TSQ Certis triple quadrupole mass spectrometer (Figure 1). The method leverages advanced ion source design and fast acquisition to deliver enhanced sensitivity, stability and throughput suitable for high-volume laboratory workflows.</p><h4><strong>Materials and methods</strong></h4><h5><strong>Liquid chromatograph – mass spectrometry&nbsp;</strong></h5><p>Samples were analyzed on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744"><strong>Thermo Scientific Vanquish Horizon UHPLC system</strong></a> coupled to the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2207"><strong>TSQ Certis MS</strong></a><strong> </strong>operated in selected reaction monitoring (SRM) mode. The mobile phase composition, analytical column details, LC gradient, and overlaid extracted ion chromatograms (EICs) are shown in Figure 3.&nbsp;</p><h5><strong>Data analysis&nbsp;</strong></h5><p>Data were acquired and processed using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/77"><strong>Thermo Scientific TraceFinder software</strong></a> (v 5.2).</p><h4><strong>Conclusions&nbsp;</strong></h4><p>A sensitive and robust LC–MS/MS method was developed for simultaneous quantification of 18 steroids in human serum using the Vanquish Horizon UHPLC and TSQ Certis MS with OptaMax Plus ion source. The method demonstrated excellent linearity, accuracy, and reproducibility across a wide dynamic range. Optimization of MS conditions, particularly vaporizer temperature, significantly improved sensitivity. Overall, the workflow delivers high sensitivity, selectivity, and long-term stability, providing a reliable solution for high-throughput steroid analysis in clinical and research settings.</p><h3><strong>4. Waters Corporation: Alkaline-hydrolysis Method for PFAS in Textiles and Textiles Products by Xevo™ TQ-S micro with ACQUITY™ UPLC™ H-Class Plus System</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009443_en_0ab4914d72.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><h5><strong>Benefits&nbsp;</strong></h5><ul><li>Alkaline hydrolysis method (EN 17681-1:2025) used for preparing textiles, coated textiles and textile products for PFAS analysis allowed the inclusion of side-chain fluorinated polymers&nbsp;</li><li>Extraction method provided significant advantages in handling complex textile matrices by creating clear extracts free from dispersions, settleable solids and particulates&nbsp;</li><li>Protected the high-value LC-MS/MS components from potential damage&nbsp;</li><li>The method improved analytical sensitivity, reproducibility and accuracy, enabled cost-effective and timeefficient PFAS analysis maintained high analytical quality even for challenging high-matrix samples</li></ul><p>Many PFAS are linked to health concerns because of their ability to interfere with the body’s hormone systems, acting as endocrine disruptors also, linked to other toxic effects. Since textiles come into direct and prolonged contact with the skin, there is an increased risk of human exposure—especially for babies and children.4 In addition, PFAS pose significant environmental concerns. These “forever chemicals” do not readily break down and can be released during manufacturing, washing and disposal, allowing them to enter water pathways and persist in the environment. As a result, testing for PFAS has become increasingly important in the textile industry to demonstrate supply chain transparency, build consumer trust and protect brand reputation.</p><p>Synthetic fluorinated chemicals are categorized into two main groups - non-polymeric PFAS and polymeric PFAS, known for their use as water and grease repellent, heat resistant finishes in textiles, upholstery, leather, apparel, carpet and other industrial applications such as packaging, firefighting foams, non-stick cookware, etc. (Figure 1). PFAS are fluorinated substances contain at least one fully fluorinated methyl or methylene carbon atom (without any H/Cl/Br/I atom attached to it), i.e. with a few noted exceptions, any chemical with at least a perfluorinated methyl group or a perfluorinated methylene group.2 PFAS are classified as long chain and short chain based on their perfluorinated carbon chain length. Short chain fluorinated polymers encompasses a diverse range of chemistries, categorized according to their generic chemical and repeating units structures. Few examples are: derivatives of fluorotelomers, perfluoropolyethers with varying carbon chain lengths and acrylates, epoxylates, urethane, silicones of repeated units based structures.3 Polyfluoroalkyl substances such as fluorotelomer alcohols (FTOHs) contain C–H bonds, rendering them more susceptible to degradation. The ethoxylates in fluorotelomers-methacrylate break down to FTOH whereas polymeric fluorinated urethanes, acrylates break down to PFCAs (non-polymeric and polymeric side chain fluorinated polymers). In contrast, perfluoroalkyl substances such as PFOS and PFOA are extremely persistent due to their fully fluorinated carbon chains.&nbsp;</p><p>In alkaline hydrolysis methods, methanolic aqueous sodium hydroxide is used to break down precursor PFAS such as FTOHs, branched side chain PFAS. This process releases terminal perfluorinated carboxylic acids (PFCAs), which can then be measured. However, the efficiency of hydrolysis varies among different PFAS compounds and the presence of interfering substances such as organic matter or other chemicals in the sample matrix can inhibit the cleavage and detection of these analytes.</p><h4><strong>Experimental</strong></h4><ul><li><strong>LC system:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/198"><strong>ACQUITY UPLC H-Class Plus System</strong></a> and SMFTN fitted with PFAS kit</li><li><strong>Column(s):</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1823"><strong>XBridge™ BEH™ C18 5 µm Column</strong></a>, 4.6 x 100 mm&nbsp;</li><li><strong>MS system: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/209"><strong>Xevo TQ-S micro Mass Spectrometer</strong></a></li></ul><h4><strong>Conclusion&nbsp;</strong></h4><p>By strictly following the critical steps of the alkaline hydrolysis method using methanolic aqueous sodium hydroxide (NaOH) both freely extractable PFAS and those previously bound within side-chain fluorinated polymers such as esters linked to fluorotelomer alcohols (n:2 FTOHs, where n = 4, 6, 8, 10, 12), can be effectively extracted. This approach also safeguards the LC-MS/MS instrumentation from blockages, damage, or contamination by preventing polymer accumulation and matrix interferences.&nbsp;</p><p>The analytical method using the ACQUITY UPLC H-Class Plus System and Xevo TQ-S micro LC-MS/MS System was found to be sensitive, reproducible and accurate, enabling cost-effective and time-efficient PFAS analysis in chemically processed textiles, coated textiles and textile products whilst maintaining high analytical quality even for challenging high-matrix samples.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 34, 2026

<h3><strong>What makes capillary LC so powerful—and why are more scientists paying attention to it?</strong></h3><p><strong>In this episode of ‪ChromatographyTalk‬, David sits down with Samuel Foster of Axcend to discuss how capillary liquid chromatography is changing the conversation around LC-MS sensitivity, solvent consumption, instrument footprint, and chromatographic efficiency.</strong></p><p>Sam explains how lower flow rates can dramatically improve electrospray ionization performance, why capillary LC can reduce solvent usage by orders of magnitude, and where the technology is already making an impact in pharmaceutical, environmental, and radiopharmaceutical workflows.</p><p>They also discuss common misconceptions about capillary LC, why older systems gave the technology a mixed reputation, and why modern advances may make now the right time for labs to take another look.</p><h5><strong>Topics Covered:</strong></h5><ul><li>What capillary LC is and how it differs from traditional HPLC</li><li>Why lower flow rates improve LC-MS sensitivity</li><li>Solvent savings and sustainability benefits</li><li>Extra-column effects and hidden performance killers</li><li>Applications in pharma, biologics, drugs of abuse, and radiopharmaceuticals</li><li>Is capillary LC ready for routine lab adoption?</li><li>The future of liquid chromatography</li></ul><h3><strong>Video Transcription</strong></h3><p>Capillary liquid chromatography has existed for decades, but improvements in pumps, fittings, system design, and LC–MS interfaces are giving the technique renewed relevance. In an interview for <i>Concentrating on Chromatography</i>, <strong>Samuel Foster</strong> discussed how capillary LC differs from conventional analytical-scale HPLC, why lower flow rates can improve electrospray ionization sensitivity, and where miniaturized LC systems could have the greatest practical impact.</p><p>Foster also explained the engineering challenges associated with operating at microliter-per-minute flow rates, the importance of controlling extra-column volume, and why applications ranging from radiopharmaceuticals to pharmaceutical analysis could benefit from dramatically lower solvent consumption.</p><h4><strong>From Undergraduate Research to Capillary Chromatography</strong></h4><p>Foster’s interest in chromatography began during his first year of university, when he joined the research group of <strong>Jim Grinias</strong> at Rowan University. What started as an undergraduate research opportunity gradually developed into a long-term focus on separation science.</p><p>According to Foster, Grinias became an important influence on his scientific career and introduced him to many aspects of chromatography, including capillary-scale separations.</p><p>That early exposure eventually shaped Foster’s doctoral research and his current work at <strong>Excend</strong>, where capillary LC plays a central role.</p><h4><strong>What Is Capillary LC?</strong></h4><p>The primary difference between conventional analytical-scale HPLC and capillary LC is the internal diameter of the chromatographic column.</p><p>Typical analytical-scale HPLC commonly uses columns with internal diameters between approximately <strong>2.1 and 4.6 mm</strong>. Capillary LC moves to substantially smaller dimensions, typically around <strong>0.1 to 0.3 mm internal diameter</strong>.</p><p>Reducing column diameter also requires a major reduction in mobile-phase flow.</p><p>An analytical-scale separation that might operate at approximately <strong>1 mL/min</strong> on a 4.6 mm column can translate to flow rates around the <strong>microliter-per-minute range</strong> when using a 0.1 mm capillary column.</p><p>The purpose is to maintain an appropriate linear velocity through the stationary phase while dramatically reducing the absolute volume of mobile phase passing through the system.</p><p>In simple terms, capillary LC combines <strong>smaller columns with much lower flow rates</strong>.</p><h4><strong>The Major Benefit: Dramatically Lower Solvent Consumption</strong></h4><p>One of the clearest advantages of capillary LC is solvent savings.</p><p>Because flow rates are orders of magnitude lower than in conventional HPLC, the total amount of mobile phase required for an analysis can be reduced dramatically.</p><p>Foster described reaction-monitoring experiments in which a reaction was followed continuously for approximately <strong>72 hours</strong> while consuming less than a microliter of total sample and less than a milliliter of mobile phase.</p><p>That difference can become especially important when a laboratory uses expensive, hazardous, or difficult-to-dispose-of solvents.</p><p>The benefits extend beyond the purchase price of mobile phases. Lower solvent consumption can also reduce:</p><ul><li>Waste generation</li><li>Disposal requirements</li><li>Storage requirements</li><li>Environmental burden</li><li>Handling of hazardous materials</li></ul><p>For laboratories operating many chromatographic systems continuously, even relatively small reductions per analysis can translate into substantial savings over time.</p><h4><strong>Why Lower Flow Can Increase LC–MS Sensitivity</strong></h4><p>Reducing flow does not necessarily mean sacrificing analytical sensitivity. When capillary LC is coupled to electrospray ionization mass spectrometry, the opposite can occur.</p><p>Foster explained that <strong>lower flow rates can improve ESI efficiency</strong>.</p><p>In conventional electrospray at analytical-scale flow rates, comparatively large droplets are generated. These droplets must undergo multiple evaporation and desolvation processes before gas-phase ions reach the mass spectrometer.</p><p>Heated gases and other source parameters are commonly used to accelerate this process.</p><p>At capillary-scale flow rates, much less solvent reaches the source. Smaller initial droplets can be generated, meaning less solvent must evaporate before ions are transferred into the gas phase.</p><p>The result can be improved ionization efficiency.</p><p>Although the precise improvement depends on the analyte and instrument configuration, Foster cited literature values suggesting approximately a <strong>four- to tenfold improvement in ESI-MS sensitivity</strong> when capillary LC is properly implemented.</p><p>This combination of reduced solvent consumption and potentially improved MS sensitivity is one of the major arguments for miniaturized LC–MS workflows.</p><h4><strong>Translating an Existing HPLC Method</strong></h4><p>Moving a method from analytical-scale HPLC to capillary LC is not necessarily as simple as reducing the flow rate.</p><p>Theoretical calculations can provide a strong starting point. Foster noted that Excend uses a method-translation calculator that allows users to enter parameters from an analytical-scale method and obtain corresponding capillary-scale conditions.</p><p>However, the calculated conditions may still require experimental optimization.</p><p>For example, a translated method may predict a flow rate of 1 µL/min, while practical testing could show that 1.5 µL/min provides the optimum separation.</p><p>Method translation can therefore bring the user close to the final conditions, but some fine-tuning is often necessary.</p><p>This is one of the practical barriers laboratories must consider when adopting capillary LC. Existing methods may not always transfer perfectly without further development.</p><h4><strong>Why Solvent Delivery Becomes More Difficult</strong></h4><p>Delivering mobile phase reproducibly at very low flow rates creates different engineering requirements from conventional HPLC.</p><p>Traditional analytical HPLC systems typically use <strong>reciprocating piston pumps</strong>. While one piston delivers solvent, another refills, allowing essentially continuous flow.</p><p>Such systems also commonly incorporate components including:</p><ul><li>Check valves</li><li>Pulse dampeners</li><li>Additional tubing and connections</li></ul><p>At analytical-scale flow rates, the additional volume created by these components may have relatively little impact.</p><p>In capillary LC, however, every microliter becomes important.</p><p>Foster explained that the Excend system uses a <strong>syringe-based pumping architecture</strong>. Instead of continuously cycling reciprocating pistons, a syringe is filled with enough solvent for the chromatographic run and then dispenses that mobile phase in a controlled step.</p><p>This approach can eliminate components such as pulse dampeners and check valves, reducing both internal system volume and the number of components that can potentially contribute to problems.</p><h4><strong>Extra-Column Volume Can Destroy a Separation</strong></h4><p>Controlling extra-column volume is one of the most important requirements in capillary LC.</p><p>At conventional analytical-scale flow rates, one microliter of additional tubing volume is generally insignificant because the mobile phase passes through it almost immediately.</p><p>At a flow rate of approximately <strong>1 µL/min</strong>, however, the same microliter represents roughly one minute of additional residence time outside the column.</p><p>That difference can severely broaden chromatographic peaks and reduce separation efficiency.</p><p>Foster therefore identified treating a capillary system like a conventional analytical HPLC system as one of the most common mistakes.</p><p>A piece of unnecessary tubing, excessive connection volume, or poorly designed fitting may have little impact in analytical-scale LC but can become a major source of dispersion in capillary chromatography.</p><p>Careful system configuration is therefore essential.</p><h4><strong>Radiopharmaceuticals as a Particularly Attractive Application</strong></h4><p>Among the potential applications discussed during the interview, Foster highlighted <strong>radiopharmaceutical analysis</strong> as one of the most promising.</p><p>The reason again comes down to solvent consumption.</p><p>Mobile phases used in radiochemical analysis may become contaminated with radioactive material and can require controlled storage until radioactivity has decayed sufficiently for disposal.</p><p>Reducing a waste volume from liters to milliliters can dramatically simplify storage, handling, and disposal.</p><p>Instead of managing large containers of radioactive mobile phase for extended periods, a laboratory could potentially reduce the waste volume by orders of magnitude.</p><p>Foster also sees broader potential across the pharmaceutical industry, particularly when standardized methods are operated on many instruments at multiple sites.</p><p>If comparable analytical performance can be obtained using substantially less solvent, the cumulative economic and environmental impact can become significant.</p><h4><strong>Lower Consumption of Challenging Mobile-Phase Additives</strong></h4><p>Miniaturization may also help laboratories reduce the amount of problematic mobile-phase additives used during LC analysis.</p><p>Foster specifically mentioned <strong>TFA and HFIP</strong>, compounds that can be required in certain chromatographic methods and that are increasingly receiving attention from an environmental perspective.</p><p>Rather than completely redesigning an established separation, capillary LC could potentially allow laboratories to retain an effective method while reducing the absolute amount of these chemicals consumed by several orders of magnitude.</p><p>This illustrates a broader advantage of miniaturization: greener chromatography does not always require replacing the chemistry of an existing method. In some cases, reducing the scale of the separation itself can substantially decrease chemical consumption.</p><h4><strong>Is Capillary LC Ready for Routine Laboratories?</strong></h4><p>Foster believes the technology is already technically ready for routine use.</p><p>Many of the problems historically associated with capillary chromatography have been reduced through improvements in instrumentation and system components.</p><p>The more difficult challenge may be regulatory acceptance.</p><p>Pharmaceutical laboratories in particular can be cautious about changing validated analytical methods. Even when method translation is scientifically straightforward, concerns over regulatory revalidation can discourage adoption.</p><p>Foster described work involving <strong>USP monograph methods</strong>, where analytical-scale procedures were translated to capillary conditions while remaining within the permitted framework.</p><p>The next important step, in his view, is wider implementation in regulated environments and greater confidence among laboratories that miniaturization does not necessarily require rebuilding a method from the beginning.</p><h4><strong>Why Some Chromatographers Remain Skeptical</strong></h4><p>Capillary LC is not a new concept. Early versions of the technology were already being investigated decades ago.</p><p>However, Foster believes that those historical experiences may actually slow current adoption.</p><p>Earlier systems could suffer from:</p><ul><li>Poor pump reproducibility</li><li>Long equilibration times</li><li>Frequent clogging</li><li>Excessive dead volume</li><li>Limited fitting technology</li><li>Insufficient robustness</li></ul><p>Researchers who used capillary LC during the 1980s, 1990s, or early 2000s may therefore remember it as technically difficult and unreliable.</p><p>Many of those limitations have since been addressed through better pumps, lower-volume connections, improved fittings, and more mature system design.</p><p>Foster’s message to users with disappointing past experiences is therefore simple: modern capillary LC deserves another look.</p><h4><strong>A Broader Trend Toward Smaller LC Columns</strong></h4><p>Capillary LC also fits within a much longer trend in liquid chromatography.</p><p>Historically, conventional HPLC frequently used <strong>4.6 mm internal-diameter columns</strong>. Over time, laboratories moved toward smaller dimensions.</p><p>Columns around 3.0 mm were promoted as solvent-saving alternatives, followed by the widespread adoption of <strong>2.1 mm narrow-bore columns</strong>. More recently, some manufacturers have continued pushing toward approximately <strong>1.5 mm internal diameters</strong>.</p><p>From Foster’s perspective, capillary LC is a logical continuation of this progression.</p><p>Chromatography has consistently moved toward smaller columns, lower solvent volumes, and more efficient separations. His ideal future would see this evolution continue until capillary dimensions become a routine rather than specialized choice.</p><h4><strong>Portable HPLC and Higher Throughput</strong></h4><p>Excend itself originated from the laboratory of <strong>Milton Lee at Brigham Young University</strong>, where one of the original ideas was to develop an HPLC system compact enough to be transported to the point of analysis.</p><p>A portable chromatograph could potentially be taken directly to a contaminated environmental site, crime scene, or other location where immediate chemical analysis would be useful.</p><p>In practice, however, Foster said customers have shown particularly strong interest in two other advantages: <strong>solvent savings and compact instrument size</strong>.</p><p>If three or four capillary LC systems can occupy the same laboratory space as one conventional analytical-scale instrument, laboratories could potentially increase chromatographic throughput without expanding their physical footprint.</p><p>The result is a different value proposition from the one originally envisioned: rather than mobility alone, compact capillary LC can combine lower solvent costs with increased instrument density.</p><h4><strong>The Future of Capillary LC</strong></h4><p>Foster sees capillary LC becoming increasingly important as laboratories seek to reduce solvent use, improve sustainability, increase LC–MS sensitivity, and make better use of laboratory space.</p><p>Its advantages are particularly compelling when solvent or sample quantities are limited, when waste is difficult to manage, or when electrospray mass spectrometry is used for detection.</p><p>Challenges remain. Methods still require thoughtful translation, extra-column volume must be controlled carefully, and adoption in regulated laboratories will require confidence that performance and compliance can be maintained.</p><p>Yet the fundamental direction of LC technology already points toward miniaturization.</p><p>For laboratories interested in evaluating the approach, Foster recommends learning from groups already working with capillary systems, reviewing the available literature, and—most importantly—gaining direct hands-on experience with modern instrumentation.</p><p>After decades of technical development, capillary LC may be moving from a specialized technique toward a practical option for routine separation science.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

 Why Capillary LC Is the Future of LC-MS Sensitivity

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<p><strong>Mass spectrometry has become a cornerstone technology across the 'omics' sciences, enabling researchers to characterise complex biological systems with unprecedented depth and confidence. As advances in instrumentation, separation science, ion mobility, tandem mass spectrometry, and computational data analysis continue to evolve, laboratories can achieve broader molecular coverage and more meaningful biological insights.</strong></p><p>This webinar explores the latest innovations shaping modern mass spectrometry workflows and examines how these technologies are advancing applications across proteomics, metabolomics, lipidomics, spatial omics, and related fields. Attendees will gain practical insight into how complementary mass spectrometry approaches are improving analytical performance, supporting systems-level biological understanding, and driving progress in biomarker discovery, disease research, and precision health.</p><h4><strong>Key Learning Objectives:</strong></h4><ul><li>Explain why mass spectrometry is a foundational technology across multiple 'omics' disciplines</li><li>Describe recent advances in mass spectrometry instrumentation, separation science, ion mobility, and data analysis</li><li>Understand how integrated mass spectrometry workflows are enabling systems-level insights into biological function</li><li>Recognise the impact of mass spectrometry innovations on disease research, biomarker discovery, and precision health</li></ul><blockquote><p><strong>Presenter:</strong> &nbsp;James McCullagh, PhD (Professor of Biological Chemistry, University of Oxford)</p><p>&nbsp;James McCullagh is a professor of biological chemistry and director of the Mass Spectrometry Research Facility at the University of Oxford. His research focuses on understanding the function of small molecules in complex biochemical systems with applications at the interface between chemistry, biology and medicine.&nbsp;</p></blockquote><blockquote><p><strong>Presenter:</strong> Vladimir Kardelis, PhD (Technical Product Manager, PerkinElmer)</p><p>&nbsp;Vladimir Kardelis is the Technical Product Manager for LC and LC/MS solutions at PerkinElmer. Since joining the company in 2019 as a Software Quality Assurance Engineer, he has progressed through an Application Scientist role to lead product development for LC/MS solutions. His work focuses on evaluating the QSight system’s detection limits and robustness across complex environmental and food samples, helping advance reliable LC/MS performance in real-world laboratory settings.&nbsp;</p></blockquote>

Technology Networks: Tech Spotlight - Mass Spectrometry 2026

Tech Spotlight: Mass Spectrometry 2026

Discover 2026 mass spectrometry innovations across omics disciplines. Learn how advances in separation, ion mobility, and data analysis drive precision health

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Trifluoroacetic acid (TFA) is an ultra-short-chain PFAS of growing concern due to its extreme persistence, high mobility, and widespread occurrence in environmental and drinking water sources. This presentation reviews the emerging regulatory attention, and the analytical challenges associated with trace-level measurement, including background contamination, matrix effects, ion suppression, and confirmatory analysis.</strong></p><p>The webinar introduces a fully automated SCIEX LC-MS/MS workflow for rapid, sensitive quantification of TFA across multiple matrices, with the flexibility to incorporate DFA and TFMS into the same analytical approach.</p><p>Validation data demonstrates excellent linearity, repeatability, accuracy, robustness, and matrix tolerance in drinking water, surface water, wastewater, mineral water, and a wide variety of food matrices. With 40 samples plus standards analyzed in less than four hours, the workflow supports high-throughput routine monitoring for environmental, food, regulatory, and contract testing laboratories.</p><h5><strong>By attending this webinar you will be able to:</strong></h5><ul><li>Implement strategies to minimize contamination and matrix effects during trifluoroacetic acid (TFA) analysis.&nbsp;</li><li>Evaluate the benefits of automated internal standard addition and automated calibration preparation.</li><li>Apply a high-throughput LC-MS/MS workflow capable of quantifying TFA down to 20 ng/L with confirmation by two MRM transitions.</li><li>Understand how trifluoromethanesulfonic acid (TFMS) and difluoroacetic acid (DFA) can be incorporated into the same analytical workflow.</li></ul><h5><strong>Who should attend?</strong></h5><p>This webinar is designed for laboratory professionals and decision-makers involved in ultra-short-chain PFAS analysis, particularly those working in environmental, drinking water, wastewater, PFAS, regulatory, and contract testing laboratories:</p><ul><li>Commercial laboratory managers, technical directors, and QA/validation specialists responsible for method implementation, data quality, ROI and regulatory readiness.&nbsp;</li><li>LC-MS/MS method developers, analytical chemists, and application scientists working on trace-level TFA, DFA, TFMS, or broader PFAS workflows.&nbsp;</li><li>Environmental scientists, researchers, and laboratory staff focused on emerging contaminants in drinking water, surface water, wastewater, mineral water, and other complex water matrices such as food.</li></ul><h5><strong>What you need to know:</strong></h5><p><strong>Start times:</strong></p><ul><li><strong>Broadcast 1:</strong> 10 am BST (London/Lisbon) / 11 am CEST (Paris/Berlin) / 2:30 pm IST (New Delhi)</li><li><strong>Broadcast 2:</strong> 10 am PDT (Los Angeles) / 12 pm CDT (Chicago) / 1 pm EDT (New York)</li></ul><blockquote><p><strong>Presenter: </strong>Carlos Bueno (Senior Aftermarket Workflow Specialist, Food, Environmental and Forensics, SCIEX EMEA)</p><p>Carlos Bueno is a Senior Aftermarket Workflow Specialist for EMEA at SCIEX with more than 20 years of experience in chromatography and mass spectrometry. After earning his bachelor’s degree in chemistry in 2001 and spending four years at the Madrid Doping Control Laboratory, he built an extensive career focused on analytical application development for food safety and environmental testing markets. Since joining SCIEX in 2023, he has supported laboratories across EMEA in implementing advanced LC-MS/MS solutions and optimizing analytical workflows, and is currently leading SCIEX EMEA's PFAS initiative.</p></blockquote></div></div></div>

SCIEX: The TFA & DFA Challenge Solved: Ultra-Sensitive Detection with End-to-End Automation

The TFA & DFA Challenge Solved: Ultra-Sensitive Detection with End-to-End Automation

Automate TFA and DFA analysis down to 20 ng/L. Join this SCIEX webinar to explore high-throughput LC-MS/MS workflows for ultra-short-chain PFAS

Automated Ultra-Short-Chain PFAS Analysis: LC-MS/MS

<p><strong>This webinar provides a foundational overview of magnetic bead-based extraction technologies used in modern bioanalytical and biopharmaceutical workflows. We will explore the scientific principles behind paramagnetic bead technology, including bead composition, surface chemistry, and the role of functionalized ligands in enabling selective capture of a wide range of biomolecules.</strong></p><p>The session will also cover common extraction workflows and how magnetic bead-based approaches compare to traditional sample preparation techniques in terms of scalability, selectivity, and reproducibility. Attendees will gain insight into how these workflows are applied across biologics research, with practical examples illustrating real-world use cases.</p><h4><strong>Key Learning Points</strong></h4><ul><li>Learn the basic principles of magnetic bead-based extraction and paramagnetic bead function.</li><li>Find how bead composition, surface chemistry, and functional ligands enable selective analyte capture.</li><li>Identify key advantages of magnetic bead workflows compared to traditional extraction methods.</li><li>Understand common workflow designs and their role in supporting downstream LC and LC-MS analysis. &nbsp;</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>Any scientists and professionals working in biopharma, life sciences, or analytical laboratories interested in learning about biomolecular extraction techniques for mAbs, ADCs, nucleic acids, mRNA, and oligonucleotides. &nbsp;</li></ul><blockquote><p><strong>Speaker:</strong> Namrata Saxena (Technical Manager, Phenomenex)</p><p>&nbsp;Namrata Saxena is the Technical Manager for the Asia-Pacific region at Phenomenex and is part of the global technical team, she provides expert support for a wide range of chromatographic challenges particularly in the characterization of biotherapeutics and emerging cell and gene therapy modalities. Her role includes delivering technical training, hosting seminars and webinars, and contributing to scientific content development. Prior to joining Phenomenex, Namrata worked as a Product Specialist at Bischoff Chromatography GmbH in Germany where she gained extensive experience in stationary phase chemistry, column manufacturing and method development domains. Namrata is based in Sydney, Australia and can be reached through e-mail or phone.&nbsp;</p></blockquote>

Phenomenex: Fundamentals First Bio Series - Understanding Magnetic Bead-Based Extraction Techniques

Fundamentals First Bio Series: Understanding Magnetic Bead-Based Extraction Techniques

Learn magnetic bead extraction fundamentals for bioanalysis. Discover how surface chemistry enables selective capture of mAbs, mRNA, and oligos for LC-MS

Magnetic Bead Extraction for Bioanalysis & LC-MS

<p><strong>High‑throughput LC‑MS/MS workflows using complex matrices are often limited by column degradation, leading to poor peak shape, rising backpressure, and unplanned downtime that impact productivity and cost. Endrix LC columns address these challenges through a hardware‑driven approach, combining proven Kinetex and Luna Omega chemistries with proprietary design to extend usable column lifetime without compromising performance. Real‑world results from a 75‑analyte pain management panel demonstrate significantly increased column lifetime while maintaining consistent chromatographic integrity. By reducing column replacement frequency and minimizing troubleshooting, Endrix enables greater instrument uptime, improved efficiency, and lower cost per injection in demanding, high‑throughput workflows.</strong></p><h4><strong>By attending this webinar, you will:</strong></h4><ul><li>Identify key factors that limit LC column lifetime in high throughput and complex matrix workflows</li><li>Understand how hardware design impacts column durability, beyond stationary phase chemistry</li><li>Implement strategies to extend column lifetime, reduce downtime, and lower cost per injection</li><li>Assess when guard columns are optional and how to simplify workflows without sacrificing performance</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>Any LC-MS/MS scientists in clinical, toxicology, and pharmaceutical laboratories, as well as analytical chemists working with complex or “dirty” matrices such as urine, plasma, and tissue samples.</li></ul><h4><strong>What you need to know:</strong></h4><p><strong>Start times: </strong>9 am CDT (Chicago) / 10 am EDT (New York) / 2 pm CEST (Paris/Berlin) / 3 pm BST (London/Lisbon)</p><blockquote><p><strong>Presenter: </strong>Grace Guo &nbsp;(Senior Technical Specialist, Phenomenex)</p><p>Grace Guo graduated from Johns Hopkins University with a Master’s degree in Material Science and Engineering. She joined the Phenomenex Technical Team in 2017 providing technical support globally. With more than 9 years of experience in SPE, HPLC and GC, she has delivered 90+ seminars and webinars globally covering the food and environmental, clinical, pharma and biopharma industries.</p></blockquote>

Phenomenex: From Downtime to Data - How Long-Life LC Columns Transform High-Throughput Labs

From Downtime to Data: How Long-Life LC Columns Transform High-Throughput Labs

Maximize LC column lifetime in high-throughput LC-MS/MS workflows. Discover Phenomenex Endrix columns to reduce downtime, cost, and matrix impact

Long-Life LC Columns: Cut Downtime in High-Throughput Labs

<h4><strong>Build Confidence in Your Laboratory Data</strong></h4><p><strong>Reliable laboratory data doesn't happen by chance, it is the result of a quality assurance program designed to detect risk, drive consistency, and support continuous improvement.</strong></p><p><strong>In this webinar, we'll explore the four foundational elements of an effective QA program: method development, analyst training, ongoing performance monitoring, and corrective action. Learn how laboratories can use precision, accuracy, control charting, trending analysis, and root cause investigations to identify issues before they impact data quality.</strong></p><p>We'll also discuss practical strategies for verifying method performance, demonstrating analyst competency, sustaining long-term process control, and using certified reference materials as independent tools to strengthen confidence in analytical results.</p><p>Whether you are building a new QA program or looking to improve an existing one, this session provides actionable guidance for creating a robust and sustainable framework for quality.</p><h5><strong>Key takeaways</strong></h5><ul><li>Build a more effective and sustainable laboratory QA program.</li><li>Use precision, accuracy, and control charting to improve data quality and identify issues early.</li><li>Apply root cause analysis and certified reference materials to strengthen performance and corrective actions.</li></ul><p><strong>Who should attend</strong></p><p>This webinar is designed for Quality Assurance (QA) and Quality Control (QC) managers, analytical chemists and laboratory scientists, laboratory managers and supervisors.</p><p>Don't miss it!</p><blockquote><p><strong>Speaker:&nbsp;</strong>Christy Abbas, PhD (Sr. Product Marketing Manager, Waters ERA)</p><p>Christy has been with Waters ERA for six and a half years. Her career spans roles at an instrument manufacturer, a biopharmaceutical laboratory, and a mining company. She earned her PhD in Applied Chemistry from the Colorado School of Mines.</p><p>Throughout her career, Christy has supported the nuclear, pharmaceutical, and chemical industries, as well as academia. Drawing on her background in analytical chemistry, product development, marketing, and sales, she is focused on expanding Waters ERA’s product portfolio to better meet evolving customer needs.</p></blockquote>

Foundations of a Sustainable, High-Performing QA Program

Build a sustainable QA program for your laboratory. Learn how control charting, root cause analysis, and reference materials drive data confidence

Sustainable Laboratory QA: Control Charts & Data Integrity

<p><strong>Accurate identification of immunopeptides is essential for understanding immune recognition in cancer. In this webinar, Reza presents how hybrid ExCID fragmentation (EAD+CID) on SCIEX ZenoTOF platforms enables the differentiation of isobaric isoleucine (Ile) and leucine (Leu) peptide variants in immunopeptidomics workflows. He also highlights the importance of chromatographic optimization for detecting naturally presented Ile/Leu sequence variants, demonstrating how advanced MS technologies can improve confidence in antigen identification.</strong></p><h4><strong>Key learning points</strong></h4><ul><li>Learn how hybrid ExCID fragmentation can distinguish isobaric Ile and Leu residues in immunopeptides</li><li>Understand how the combination of EAD and CID improves peptide sequence characterization and identification confidence</li><li>Discover why chromatographic optimization is critical for detecting naturally presented Ile/Leu variants in the immunopeptidome</li></ul><blockquote><p><strong>Speaker: </strong>Reza (Mohammadreza) Dorvash, Ph.D. (Postdoctoral Researcher, Bio21 Molecular Science and Biotechnology Institute)</p></blockquote>

SCIEX: Distinguishing Ile/Leu variant ligands in the immunopeptidome using hybrid ExCID fragmentation on SCIEX accurate mass instrument

Distinguishing Ile/Leu variant ligands in the immunopeptidome using hybrid ExCID fragmentation on SCIEX accurate mass instrument

Differentiate Ile/Leu variants in immunopeptidomics using hybrid ExCID (EAD+CID) fragmentation on SCIEX ZenoTOF. Improve antigen identification confidence

Distinguishing Ile/Leu Variants with SCIEX ExCID MS

<div><h4><strong>Tiny Plastics, Big Questions: Advances in Microplastics &amp; Nanoplastics Analysis</strong></h4><p><strong>Plastic particles are now recognized as widespread environmental contaminants, ranging from nanoplastics (&lt;1 µm) to microplastics (&lt;5 mm), and have been detected in drinking water, food, air, soil, and consumer products. As concerns over human exposure continue to grow, there is an increasing need for analytical technologies that can accurately detect, size, separate, and characterize these particles across a broad size range.</strong></p><p>This webinar explores complementary analytical approaches for comprehensive plastic particle characterization. The first presentation highlights light scattering-based techniques for nanoplastics, demonstrating how Dynamic Light Scattering (DLS) enables rapid screening of particle size and concentration, while Asymmetrical Flow Field-Flow Fractionation coupled with Multi-Angle Light Scattering (AF4-MALS) provides high-resolution size separation, absolute particle sizing, and fraction collection for downstream chemical identification.</p><p>The second presentation focuses on high-throughput methodologies for microplastics in drinking water, illustrating approaches that detect, count, size, and characterize particles from a variety of water sources. Case studies will examine commercially available drinking water products and discuss how packaging materials, storage conditions, and temperature can influence measured microplastic levels.</p><p>Together, these presentations demonstrate how complementary analytical technologies provide a more complete understanding of plastic particle populations across the nano- and microscale, enabling researchers to address emerging environmental and human exposure questions with greater confidence.</p><blockquote><p><strong>Presenter:&nbsp;</strong>Udayabagya (Bud) Halim (Sr. Product Marketing Analyst, Waters Corporation)</p><p>Bud Halim is a senior marketing analyst at Waters, where he applies his background in materials science and biochemistry to scientific and technical marketing. He holds a PhD in physical chemistry from UCLA. Prior to joining Waters, Bud worked as an applications scientist, supporting customers across chemistry and manufacturing environments. At Waters, he supports the light scattering and particle analysis portfolio, translating advanced characterization technologies into practical solutions for polymer, biopharma, and other scientific and industrial applications.</p></blockquote><blockquote><p><strong>Presenter:&nbsp;</strong>Karessa White (Sr. Market Applications Scientist, Waters)</p><p>Dr. Karessa White is a Sr. Market Application Scientist at Waters Corporation. She obtained her Ph.D. from the University of Florida in Communication Sciences and Disorders emphasizing auditory neuroscience. After spending time serving her country as a United States Army Medical Service Officer, Karessa now leverages her expertise and training in molecular biology and analytical science to help biotherapeutic developers create safe and stable drugs.</p></blockquote></div>

Waters Corporation: Tiny Plastics, Big Questions: Advances in Microplastics & Nanoplastics Analysis

Tiny Plastics, Big Questions: Advances in Microplastics & Nanoplastics Analysis

Discover advanced microplastics and nanoplastics analysis using DLS and AF4-MALS. Learn how high-throughput methods characterize particles in drinking water

Microplastics & Nanoplastics Analysis: DLS & AF4-MALS

<p><strong>Gene expression profiling is a cornerstone of clinical and translational research, providing critical insights into disease biology, biomarker discovery, patient stratification, and therapeutic response. Yet many transcriptome analysis workflows remain limited by complex library preparation, long turnaround times, and inconsistent performance with precious or degraded clinical research samples.</strong><br><strong>Join this webinar to learn how the Ion AmpliSeq™ Transcriptome portfolio enables fast, sensitive, and reproducible transcriptome analysis designed to meet the demands of translational research.</strong></p><p>We'll explore how targeted transcriptome sequencing can quantify the expression of more than 20,000 RefSeq genes from as little as 10 ng of input RNA, including challenging FFPE specimens commonly used in clinical research, while delivering results in as little as two days.</p><p>Whether you're investigating disease mechanisms, researching biomarkers, or developing gene expression signatures, discover how Ion AmpliSeq™ Transcriptome solutions can help accelerate your research with a fast, scalable, and easy-to-implement NGS workflow.</p><p>For Research Use Only. Not for use in diagnostic procedures.</p><blockquote><p><strong>Presenter: &nbsp;</strong>Phil Waggoner, PhD (Director, Chemistry &amp; Data Analytics R&amp;D, Thermo Fisher Scientific)</p><p>Phil Waggoner, Ph.D. is a genomics professional with experience supporting the development and adoption of next-generation sequencing (NGS) technologies for research and clinical applications. At Thermo Fisher Scientific, he collaborates across technical, commercial, and customer-facing teams to implement NGS solutions that support precision medicine. Phil earned his Ph.D. in Applied Physics from Cornell University, where his research focused on semiconductor technologies and biosensors, and he has authored several peer-reviewed publications in nanotechnology and genomics. His background spans scientific research, technology development, and commercial strategy, providing him with a broad perspective on the opportunities and challenges of applying NGS in clinical settings.</p></blockquote>

Thermo Fisher Scientific: Simplifying Transcriptomics for Clinical Research with Ion AmpliSeq™ Technology

Simplifying Transcriptomics for Clinical Research with Ion AmpliSeq™ Technology

Simplify transcriptomics with Ion AmpliSeq technology. Quantify 20,000+ RefSeq genes from 10 ng RNA or FFPE samples with a fast 2-day NGS workflow

Ion AmpliSeq Transcriptomics: Fast NGS for FFPE Samples

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><h4><strong>Leverage unique capabilities and powerful tools to streamline sample analysis.</strong></h4><p>Join this webinar to discover the multiple tools in Thermo Scientific Chromeleon software that drive sample throughput from acquisition to data processing and deliver reduced errors through advanced automation.&nbsp;</p><h5><strong>Why attend</strong></h5><p>Uncover the Chromeleon software features that drive sample throughput and deliver unique capabilities in combination with liquid chromatography systems such as the Thermo Scientific Vanquish UHPLC systems. In this webinar, you will see how Chromeleon software can help:</p><ul><li>Increase sample throughput through streamlined workflows</li><li>Leverage the full capabilities of your LC systems to drive productivity</li><li>Deliver automation to common analyses to reduce errors</li></ul><p>Through practical examples and live software demonstrations, attendees will learn how to improve day-to-day laboratory efficiency and reduce errors.</p><h5><strong>What you will learn</strong></h5><p>By attending, participants will be able to:</p><ul><li>Streamline analysis in Chromeleon software from acquisition to result</li><li>Understand and leverage unique capabilities to get more from your LC systems</li><li>Drive automation in common workflows to reduce errors</li></ul><p>Save your spot to discover practical ways to optimize LC system workflows with Chromeleon software.</p><p><i>Cannot attend the live broadcast? Please register anyway to automatically receive the link to the recording.</i></p><blockquote><p><strong>Presenter: </strong>Darren Barrington-Light (Senior Manager, Product Marketing, Thermo Fisher Scientific)</p><p>Darren Barrington-Light’s background includes over 25 years of experience in the field of analytical chemistry, chromatography hardware and computer software. Having worked in chromatography throughout his career, Darren has a unique understanding of the requirements of organizations that rely on chromatography data to manage their day to day operations. As capabilities have advanced, he has worked with clients across multiple industries to show them how software solutions can help them to improve their day to day operations and drive fast, informed decisions.</p></blockquote></div></div></div>

Thermo Fisher Scientific: Tips to power up your LC with Chromeleon software

Tips to power up your LC with Chromeleon software

Optimize LC workflows with Thermo Scientific Chromeleon software. Learn how automation drives throughput and reduces errors on Vanquish UHPLC systems

Chromeleon Tips: Vanquish LC Workflows

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Building on the foundations of LC method development, this webinar explores practical strategies for optimizing chromatographic separations through smarter mobile phase selection, column selectivity, and sample handling. Learn how small changes in solvent composition, pH, buffer selection, and stationary phase chemistry can significantly improve resolution, reproducibility, and method robustness.</strong></p><p>The session will also examine common but often overlooked causes of method variability, including mobile phase stability and sample adsorption, and share practical approaches to improving analyte recovery and overall analytical performance.</p><p>Whether you're developing new methods or troubleshooting existing ones, this webinar will provide practical insights and actionable tips to help you achieve more reliable and efficient LC separations.</p><h5><strong>Who Should Attend?</strong></h5><p>This webinar is ideal for scientists and laboratory professionals involved in liquid chromatography method development, optimization, transfer, or troubleshooting, including:</p><ul><li>Analytical chemists and chromatography specialists</li><li>HPLC and UHPLC users</li><li>Pharmaceutical, biopharmaceutical, food, environmental, and chemical testing professionals</li><li>Method development, validation, and quality control scientists</li><li>Researchers looking to improve separation performance, analyte recovery, and method reproducibility</li><li>Laboratory professionals experiencing challenges related to selectivity, mobile phase selection, sample handling, or unexpected LC method variability</li></ul><p>The session will be valuable for both newer LC users seeking practical guidance and experienced chromatographers looking to strengthen method robustness and troubleshoot difficult separations more efficiently.</p><blockquote><p><strong>Presenter: </strong>Detlev Lennartz (Product Manager, Thermo Fisher Scientific)</p><p>Dr. Detlev Lennartz joined Thermo Fisher Scientific in 2011 and has 3 years of active end customer sales experience and worked previously 12 years as a worldwide product manager for chromatography consumables (HPLC columns, GC columns, SPE cartridges, TLC products and vials and closures).</p><p>In his current position at Thermo Fisher Scientific Detlev works as global product manager for the branded sample handling portfolio (autosampler vials, closures and well plates) close together with a team of specialists, focusing on developing and launching new and innovative products, business development and technical support and trainings for all chromatography consumables.</p></blockquote><blockquote><p><strong>Presenter: </strong>Adham Ahmed, PhD. (Global Product Manager, Thermo Fisher Scientific)</p><p>Adham Ahmed has over 15 years of experience in chromatography, specialising in particle development and functionality for small molecules applications. As Global Product Manager at Thermo Fisher Scientific, he oversees the Hypersil BDS, Hypersil Classical, Acclaim and Hypercarb HPLC column portfolio.</p><p>His experience across research &amp; development, applications support, and product management has provided him with a broad understanding of chromatographic method development, laboratory workflows, and customer needs across a wide range of analytical applications.</p></blockquote></div></div></div>

Thermo Fisher Scientific: Tips and Tricks II - Optimising LC Column Separation

Tips and Tricks II: Optimising LC Column Separation

Optimize LC column separation and method robustness. Learn how mobile phase selection, pH, and column selectivity improve resolution and analyte recovery

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